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Translation Initiation: Reconstituted Systems and ...

eIF2,. eIF5,. and. eIF5B. 5.1. Buffers Buffer D 20 mM Tris, pH 7.5, 2 mM DTT, ... 2 mM DTT, 0.1 mM EDTA, 5% glycerol Most native eIF2 and smaller amounts of ...

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