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[爆卦]HEK293T是什麼？優點缺點精華區懶人包
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#1293T - 維基百科，自由的百科全書
293T（或HEK293T）是一種由HEK 293細胞系衍生的人類細胞系，表達SV40大T抗原（英語：SV40 large T antigen）的突變體。常用於生物蛋白的表達，以及重組逆轉錄病毒的 ...
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#2293T | ATCC
The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T- ...
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#3Cellosaurus cell line HEK293T (CVCL_0063) - Expasy
Cell line name, HEK293T · Synonyms, Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo · Accession ...
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#4HEK293T Cell Line - an overview | ScienceDirect Topics
HEK293T cell line (ATCC). 2. 25 cm 3 cell culture flasks with filter cap. 3. Dulbecco's modified eagle medium (DMEM), supplemented with 10% fetal bovine ...
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#5CellSensor™ ISRE-<i>bla</i> HEK293T Cell Line - Thermo ...
Interferon cell signaling is a critical pathway involved in viral defense and autoimmune disease. The CellSensor ISRE-bla HEK 293T cell line is responsive ...
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#6HEK293T Cell Line - Horizon Discovery
The HEK293T cells have been tested for viability and verified to contain no. Mycoplasma contamination. Protocol for culturing HEK293T cells. Components for 10% ...
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#7Design and Testing of Vector-Producing HEK293T Cells ...
由 DH Bae 著作 · 2020 · 被引用 5 次 — HEK293T cells were originally generated by stably transfecting HEK293 cells with a plasmid expressing a temperature-sensitive version of the ...
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#8T Antigen Coding Region
HEK293T cells by transfecting them with a recombinant ... HEK293 cell line, lentiviral vector titers obtained using HEK293T.
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#9OptiPEAK HEK293t: Chemically Defined Adherent ... - InVitria
OptiPEAK HEK293t is optimized for chemically defined blood-free expansion of adherent HEK 293t cells for production of viral vectors used in gene therapy or ...
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#10Transient transfection protocol for HEK293T cells - EuroMAbNet
In our experience, HEK293T cells are easily transfected and the percentage of transfection can be around 80% by using the present protocol. Cell line: HEK293T - ...
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#11Human ACE2 Stable Cell Line - HEK293T - Creative ...
This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HEK293T cells, followed by stable cell selection.
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#12HEK293T Whole Cell Lysate | SCBT
HEK293T (human human embryonic kidney) Whole Cell Lysate is provided as a Western blotting positive control.
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#13Human wild-type HEK-293T cell line (ab255593) | Abcam
Overview. Product name. Human wild-type HEK-293T cell line. See all HEK293 lysates. Description. Wild-type HEK-293T cell line. Parental Cell Line. HEK293T ...
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#14HEK293T host cell protein ELISA kit - ENZ-KIT162 - Enzo Life ...
Sensitive measurement of HEK293T host cell protein; Low intra-assay variability allows for streamlined testing; High throughput format with results in 6 ...
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#15Propagation of TLA-HEK293T - UCSC Genome Browser
Ordering Information. TLA-HEK293T can be ordered from Open Biosystems (Part of Thermo Fisher Scientific). Name: HEK293T, human embryonic kidney.
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#16HEK293T/AT1 Stable Cell Line - GenScript
Angiotensin receptor 1 (AT1 receptor) is a member of angiotensin receptors which are responsible for the signal transduction of the main effecter hormone.
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#17GAPDH Knock Down HEK293T Cell Line (GAPDH-KD)
Human embryonic kidney 293T (HEK293T) cells with the GAPDH gene knocked down by shRNA.
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#18Hek293t | Sigma-Aldrich
hek293t · VirusExpress ® 293T Lentiviral Production Cells · EX-CELL ® CD HEK293 Viral Vector Medium · Support · Orders · Company · Social Media · MilliporeSigma.
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#19Adherent HEK293t Cells Cultured in the iCELLis® Bioreactor ...
Chemically defined media that is optimized specifically for the adherent HEK293t-based viral vector production in the iCELLis bioreactor. · Developed to ...
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#20HEK293T - Wikidata
Hek293T ; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo. In more languages. Spanish. HEK293T.
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#21ACE2-3xFLAG-HEK293T stable cell line - ACE Biolabs.com
ACE2-3xFLAG-HEK293T stable cell infected by Pseudovirus-SARS-CoV-2 (ACE #PV031), can be observed the expression of green fluorescent protein and detected ...
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#22horizon discovery biosciences limited hek293t cell line hcl4517
HORIZON DISCOVERY BIOSCIENCES LIMITED HEK293T CELL LINE HCL4517 ... This product was recently added by customer request, and is available for your convenience. We ...
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#23hek293t - List of Frontiers' open access articles
This page contains Frontiers open-access articles about hek293t. ... ROP18-Mediated Transcriptional Reprogramming of HEK293T Cell Reveals New Roles of ROP18 ...
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#24Kinetics and Specificity of HEK293T Extracellular Vesicle ...
Further, the selectivity of EV uptake was quantified in vitro, and we found that HEK293T EVs were internalized at higher quantities by cells of ...
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#25HEK293: An Essential Human Cell Line with a Unexpected ...
Both HEK293 and HEK293T represent adherent cell lines that form a stable connection with coated cell culture dishes. This limits their volume to ...
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#26Exosomes derived from HEK293T cells interact in an efficient ...
Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T ...
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#27ENCBS634AAA / cell line - HEK293T - encode
Homo sapiens HEK293T cell line. Description: embryonic kidney that expresses SV40 large T antigen, HEK293 (ATCC number CRL-1573) is the parental cell line.
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#28HEK293T Luciferase stable cells (RFP Puromycin) - Gentarget
HEK293T cells constitutively the firefly luciferase gene, containing RFP fluorescent marker and Puromycin resistance.
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#29Transfection of Human Embryonic Kidney Cell-line 293T ...
In the present study we investigated whether. HEK293T cells are good recipient cells for transfection of mammalian expression plasmid DNAs with Metafectene Pro.
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#30Establishment of a HEK293T cell line able to ... - SciELO Chile
Background: Using recombinant adeno-associated virus 2 (rAAV-2), we attempted to establish a HEK293T cell line that is able to site-specifically integrate ...
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#31COVID-19 coronavirus receptor stable cell lines - GeneCopoeia
GeneCopoeia carries a HEK293T cell line expressing ACE2, the receptor needed for SARS-CoV-2 infection in humans.
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#32Establishment of a HEK293T cell line able to ... - SciELO Chile
HEK293T cells were co-infected with rAAV-2/EGFP-GDNF and rAAV-2/SVAV2 virus separately at 1 x 10 4,1 x 10 5,and 1x10 6 of multiplicity of infection (MOI). The ...
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#33Adaptation of African swine fever virus to HEK293T cells - Wang
Interestingly, the ASFV wild-type strain ASFV-HLJ/18 can infect HEK293T cells and replicate at a low level. After continuous passaging ...
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#34HEK293 Cells: Background, Applications, Protocols, and More
HEK293T cells are a popular derivative of the original HEK293 parent cell line. The HEK293T genome contains the SV40 large T antigen, which enables them to ...
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#35untransfected cell lysate (negative control) – LY500001
HEK293T cell lysate - untransfected cell lysate (negative control)
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#36TempoMito™-HEK293t - Tempo Bioscience
HEK293t cell line is one of the most widely used human immortalized cell lines in research and drug development. The cells are very easy to work with and ...
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#37HEK293 Cells - A Guide to This Incredibly Useful Cell Line
So, are HEK293T cells the same thing? Not exactly. The 'T' in the name of this daughter cell line comes from the incorporation of the SV40 large ...
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#38Human ACE2 Stable Cell Line - HEK293T (CSC-RO0641)
This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK293T is derived from HEK293 and is commonly used in ...
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#39Cell Banking of HEK293T cell line for clinical-grade lentiviral ...
HEK293T cell banks were used to manufacture clinical-grade lentiviral particles for Chimeric Antigen Receptor T-cell based clinical trials.
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#40HEK293T Host Cell Protein ELISA Kit | Canopy Biosciences
Use our HEK293T HCP ELISA kit for the detection and quantification of host cell proteins in HEK293T-derived biologics. Click to view our kit components!
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#41HEK293T Cells with TFAM Disruption by CRISPR-Cas9 as a ...
The aim of this study was to generate a new in vitro model by CRISPR-Cas9 system by editing the TFAM locus in HEK293T cells. Among the resulting single-cell ...
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#42HEK293T cell lines defective for O-linked glycosylation - PLOS
Here we describe derivatives of the HEK293T cell line that are defective in their ability to generate mucin-type O-linked glycosylation.
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#43293T cells for lentiviral packaging - Takara Bio
293T cells that transfect easily and support high-level expression of lentiviral proteins. The Lenti-X 293T Cell Line was clonally selected to meet these ...
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#44HEK293T
experimental factor. material entity. specimen. cellular component. cell. cultured cell. epithelial cell derived cell line. HEK293T.
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#45ambr-15-optimization-of-the-hek293t-suspension-cultivation ...
HEK293T cells are commonly used as a workhorse cell line for viral vector production for cell and gene therapy applications.
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#46hACE2-GFP-HEK293T Stable Cell Line (CAT#: VCeL-Wyb032)
ACE2-GFP gene was integrated into the chromosomes of HEK293T cells to form an engineered cell line, hACE2-GFP-HEK293T, that stably expresses hACE2-GFP on ...
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#47Measured Effects of Wnt3a on Proliferation of HEK293T Cells ...
This study aims to examine the effects of Wnt signaling on proliferation of cultured HEK293T cells. Cells were incubated with Wnt3a, and the activation of ...
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#48RNA-seq HEK293T recent datasets - Biostars
We have both polyA plus and minus RNAseq on HEK293T cells, published recently : https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85161.
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#49NGVB - Reagent Repository View
Name, HEK293T. Reagent Type, Cell Line. Vector Classifications, Lentivirus Retrovirus. Keywords. Cell Line Origin, Human Embryonic Kidney Cells.
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#50Increase HEK293T Single-Cell Clonal Outgrowth Under ...
The HEK293T cell line is increasingly used in biotherapeutic protein production due to its human origin, but often becomes very fragile after transfection.
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#51HEK293T CAGA12-Luc/TK Renilla cell line - Ximbio
The human embryo kidney cell line HEK293T modified for use as a reporter cell line for Activin and TGF-beta signalling.
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#52HEK293T Parental Line | Product Catalog | Multispan, Inc
HEK293T parental line general information. Latest stable cell lines, division-arrested cells, and membranes. View product information.
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#53Raman spectroscopic study of transfected HEK293T cells
We have obtained Raman spectra from HEK293T cells expressing a single transgene in order to analyze whether this technique could be of interest as a tool ...
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#54Effect of FBS on the adherent property of HEK293T cells. a ...
Human embryonic kidney 293T (HEK293T) cells are used in various biological experiments and researches. In this study, we investigated the effect of cell culture ...
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#55Effect of the Proteasome Inhibitor Epoxomicin on Intracellular ...
To test whether the proteasome is involved in their formation, HEK293T cells were treated with epoxomicin (0.2 or 2 μM) for 1 h and ...
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#56Human ESD knockout HEK293T cell line from Abcam plc
Human ESD knockout HEK293T cell line by Abcam plc. Manufacturer Abcam plc | Model: ab266360. Be the first to review this product.
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#57Protocol 2 – Producing lentivirus in HEK293T cells using a 2
Protocol 2 – Producing lentivirus in HEK293T cells using a 2 nd. Generation lentiviral system. Before any work begins, you must have contacted your ...
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#58The Scattered Twelve Tribes of HEK293 - Biomedical and ...
HEK293T – HEK293T is a HEK293 variant that expresses a ... HEK293FT cells were cloned from the HEK293T cell line and adapted to commercial ...
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#59Human ACE2 Stable Cell Line - HEK293T - rapidmicrobiology
This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HEK293T cells, followed by stable cell selection.
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#60ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 ... - Nature
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 20191,2 and is responsible for the coronavirus disease 2019 ...
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#61HEK293T-ACE2-TMPRSS2 - NIBSC
Product Number, 101008. Product Description, HEK293T-ACE2-TMPRSS2. Type of Standard, NIBSC Repository - Research Use Only.
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#62The translational response of the human mdm2 gene in ...
HEK293T cells (ATTC, CRL-11268) were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma) supplemented with 10% fetal calf serum (Brunschwig), ...
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#63HEK293과 HEK293T의 차이가 뭔가요? > BRIC
안녕하세요 ~이번에 animal cell을 키우는 실험에서HEK293 cell과 HEK293T cell을 사용하는 데 이 두개...
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#64Adaptation of African swine fever virus to HEK293T cells ...
Interestingly, the ASFV wild-type strain ASFV-HLJ/18 can infect HEK293T cells and replicate at a low level. After continuous passaging ...
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#65Ribosome profiling of HEK293T cells... | F1000Research
The two HEK293T cell lines each express a version of the F9 gene, both of which are translated into identical proteins in terms of their amino acid sequences.
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#66Production of cGMP compliant aav in suspension adapted ...
HEK293T cells were adapted for suspension culture in F17 Freestyle medium and transfected with PEI using a two plasmid system for AAV-6.
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#67HEK Cell Splitting and Maintenance | Şen Lab
Human embryonic kidney cells 293 (HEK-293) and 293T cells (those that contain SV40 Large T-antigen) show a reliable growth and have a propensity for ...
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#68What is the Difference Between HEK293 and HEK293t
HEK293t is a human cell line that expresses a mutant version of the SV40 large T antigen. HEK293t was created in Michele Carlos's laboratory at ...
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#69TRANSFECTION OF HEK293T CELLS INTO 24-‐WELL PLATES
with the HEK293T cell line. PEI is a very cost effective transfection factor which allows for external. DNA to be endocytosed, ...
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#70RNA-Seq HEK293T cells transfected with plasmids ...
Abstract. HEK293T cells were transfected with plasmids expressing the full-length SARS-CoV-2 Nucleocapsid protein with an without the R203K/ ...
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#71Optimized Chemically Defined HEK293t Media for Viral Vector ...
Efficient viral vector production is a key element for gene therapy manufacturing. To ensure the best production, special care must be taken ...
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#72Transfecting HEK293T cells for virus production - Sanquin
Transfecting HEK293T cells for virus production. Day 0. Seed HEK à next day +/- 80 % confluent. Day 1. Transfection with Fugene.
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#73Limited, but potentially functional translation of non-coding ...
Limited, but potentially functional translation of non-coding transcripts in the HEK293T cellular cytosol.
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#74HEK-293T细胞ATCC CRL-3216 - 生物风
HEK-293T细胞,HEK-293T细胞株,ATCC CRL-3216细胞,HEK293T细胞购买价格1800元,人体肾脏细胞系培养基,细胞形态图片，培养条件，冻存条件请见网页，本细胞可以用于转染 ...
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#75Human ACE2 overexpression stable HEK293T cell lines
GeneMedi's human ACE2 overexpression stable HEK293T cell lines (Cat.GM-SC-293T-hACE2-01)is a efforter cell in SARS-CoV-2(2019nCoV) Pseudotyped Virus Based ...
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#76AAV Production in HEK293T Cells - Addgene
Adherent HEK293T cells (ideally AAV-293T clones) · T-150 flask (Corning, 150 cm 2 ) · Five chamber cell stack (CF5, Corning, 3180 cm 2 ) · Heat- ...
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#77Function of Hsf1 in SV40 T‑antigen‑transformed HEK293T cells
The shRNA was transiently transfected into HEK293T cells to silence the expression of the HSF1 gene. Cell colony formation, MTT and cell cycle ...
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#78Using HEK293T Expression System to Study Photoactive ...
We report here an easy-to-use method to express and study Arabidopsis cryptochrome in HEK293T cells. Our results indicate that Arabidopsis cryptochromes ...
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#79Fatty acids suppress voltage-gated Na+ currents in HEK293t ...
HEK293t cells successfully transfected with hH1α channels had voltage-activated Na + currents with fast activation and fast inactivation kinetics (Fig. 1A).
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#80What the HEK293t? - Davis Lab | MIT
... Thermo just installed a stack of new incubators (pretty, right?), which we will use to culture mammalian cell lines including HEK293t.
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#81Optimization of gene delivery to HEK293T cells by ...
Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology. C. Madeira, S. C. Ribeiro, ...
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#82Excellent proliferation of HEK293T cells for the production of ...
Excellent proliferation of HEK293T cells for the production of retroviral vectors in the chemically defined InVitrus VP-6 medium. · Cell Culture Technologies now ...
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#83AAV Production in HEK293T Cells V.1 - Protocols.io
This protocol is used for AAV production in HEK293T cells. To see the full abstract and additional resources, visit the Addgene protocol ...
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#84Analysis of Wnt signaling β-catenin spatial dynamics in ...
A single compartment Wnt pathway model is compared with a simple β-catenin two compartment model to investigate Wnt3A signaling in HEK293T cells. When protein ...
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#85HEK293T Transfection Protocol
Make HEK293T growth media: Reagent. Manufacturer. Catalog No. Volume. GlutaMAX. Gibco. 35050. 5 mL. Penicillin-Streptomycin Gibco. 15140-122.
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#86Double immunofluorescent labeling of HEK293T cells ...
Supplemental Figure 1: Double immunofluorescent labeling of HEK293T cells transfected with WT human tau or different human tau mutants showing ...
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#87Catalytic eDHFR Acylation in HEK293T Cells - Thieme Connect
Catalytic eDHFR Acylation in HEK293T Cells. Significance: Live-cell protein modification using abiotic catalysis is an effective approach to study.
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#88Team:Freiburg/Cell culture - iGEM 2017
HEK293T is a broadly used cell line that is especially easy to keep in culture and can be genetically manipulated by simple transfection methods. We used this ...
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#89Nicotinic Acetylcholine Receptor Dependent Effects of ...
Nicotinic Acetylcholine Receptor Dependent Effects of Nicotine on HEK293T and HBO Cells. A thesis submitted in partial fulfillment for the requirements of ...
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#90293T Cells Transfection Reagent (CRL-11268) - Altogen ...
Ordering: To place an order please use Add to Cart button (account not required). Transfection Reagent for 293T/17 Cells (Embryonic Kidney Cells, CRL-11268).
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#91CD20 antibody | HEK293T - Intelligent Search Engine iSpyBio
CD20 antibody | HEK293T. ' CD20(MS4A1) (NM_021950) Human Over-expression Lysate from OriGene. Images: 1; Applications: 0; Publications: 0; Reviews: 0.
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#92CD33 stable cell line, HEK293T, Red fluorescent protein (RFP)
Stable, recombinant clonal HEK293T cell line constitutively expressing full length human CD33 protein. Surface expression of CD33 was confirmed by flow ...
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#93中国科学院苏州生物医学工程技术研究所
论文题目: Establishment of a HEK293T cell line able to site-specifically integrate and stably express GDNF by rAAV-2 vector. 论文题目英文:.
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#94HEK293 Cell Line Origins, Cytogenetics, and Expression
HEK 293 cells are popular for their ease of growth and transfection (HEK293 Transfection Kit), making them a common cell culture in cancer research.
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#95[PDF] HEK293T cell lines defective for O-linked glycosylation
Derby derivatives of the HEK293T cell line that are defective in their ability to generate mucin-type O-linked glycosylation are described ...
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宗教牌👍🏻
（利申：我日日用HEK293T和人類胚胎幹細胞，大哥哥。）