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[爆卦]HEK293T ATCC是什麼？優點缺點精華區懶人包
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#1293T | ATCC
The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T- ...
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#2Cellosaurus cell line HEK293T (CVCL_0063) - Expasy
Resource Identification Initiative, To cite this cell line use: HEK293T ... https://www.atcc.org/en/support/technical-support/faqs/growth-medium-for-crl- ...
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#3293T cell line human 12022001 | Sigma-Aldrich
HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 ...
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#4293T - 維基百科，自由的百科全書
293T（或HEK293T）是一種由HEK 293細胞系衍生的人類細胞系，表達 的突變體。 · 293T細胞系最初在史丹福大學Michele Calos的實驗室中製造，是一種穩定轉染了編碼 · 在製造 ...
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#5HEK293T Cell Line - an overview | ScienceDirect Topics
HEK293T cell line (ATCC). 2. 25 cm 3 cell culture flasks with filter cap. 3. Dulbecco's modified eagle medium (DMEM), supplemented with 10% fetal bovine ...
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#6Human wild-type HEK-293T cell line (ab255593) | Abcam
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. Culture medium: DMEM ( ...
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#7Design and Testing of Vector-Producing HEK293T Cells ...
We next deleted the SV40 T antigen-encoding sequences in HEK293T cells ... HEK293T cells (CRL-3216, ATCC, Manassas, VA, USA) and HEK293 ...
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#8HEK293T/17 SF Suspension Cells from ATCC - Biocompare
Product Specs; ItemHEK293T/17 SF Suspension Cells; CompanyATCC; Price Pricing InfoSupplier PageView Company Product Page; Catalog NumberACS-4500 ...
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#9ATCC® CRL-3216™ 293T, American Type Culture Collection
The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T- ...
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#10Hek293t (ATCC) | Bioz | Ratings For Life-Science Research
ATCC hek293t. Effect of conjugation on small interfering RNA (siRNA) inhibition. ( a ) Two Hek293t, supplied by ATCC, used in various techniques.
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#11HEK293T/17 SF; Suspension Cells - ATCC - LGC Standards
Purchase online ATCC - HEK293T/17 SF; Suspension Cells. High Quality CRMs, Reference Materials, Proficiency Testing & More at LGC Standards.
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#12T--NYMU-Taipei--protocol-hek293-culture.pdf - iGEM 2018
HEK293 cells (Human embryonal kidney cells (HEK293, ATCC [Cat. No. CRC-1573]). Cell culture. Medium 90% Minimum Essential Medium [Invitrogen/Gibco, Cat. No.
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#13293T Cells | Thermo Fisher Scientific - US
These cells are isolated from human embryonic kidneys (HEK). The 293T cells are transformed with large T antigen. Tissues, Kidney. Phenotype, Either. Primary ...
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#14Human ACE2 Stable Cell Line - HEK293T - Creative ...
This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HEK293T cells, followed by stable cell selection.
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#151 SUPPLEMENTARY METHODS Cells and cell culture IMR90 ...
(ATCC HTB-37), MCF7 (ATCC HTB-22), HEK293T (ATCC CRL-3216), and SW480-ADH cells were cultured in DMEM plus 10% FBS (both from Life Technologies, Carlsbad,.
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#16RRID:CVCL_1926 - SciCrunch | Research Resource Resolver
Proper Citation: (ATCC Cat# CRL-11268, RRID:CVCL_1926). Description: Cell line HEK293T/17 is a Transformed cell line with a species of origin Homo sapiens.
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#17293T cells for lentiviral packaging - Takara Bio
Getting the most from your lentiviral packaging system requires a host HEK 293T cell line that is easily transfected and supports high-level expression of ...
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#18HEK293 Cells: Background, Applications, Protocols, and More
Image credit: ATCC ... HEK293 Vs. HEK293T: What's the difference? HEK293T cells are a popular derivative of the original HEK293 parent cell line.
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#19ENCBS634AAA / cell line - HEK293T - encode
Homo sapiens HEK293T cell line. Description: embryonic kidney that expresses SV40 large T antigen, HEK293 (ATCC number CRL-1573) is the parental cell line.
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#20PGC-1a/ERRa Pathway HEK-293T Cell Lines - Kerafast
HEK293T /17 Cells expressing endogenous estrogen-related receptor alpha (ERR alpha) transduced with peroxisome ... Cell Type: HEK 293T/17 (ATCC #CRL-11268).
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#21Human Embryonic Kidney 293 - Beckman Coulter
Additional names include: HEK-293, 293 cells or HEK cells (ATCC* CRL-1573). ... and HEK293T is an important line for propagating retroviral vectors.
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#22293T - German Collection of Microorganisms and Cell ...
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
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#23HEK-293T细胞ATCC CRL-3216 - 生物风
HEK-293T细胞,HEK-293T细胞株,ATCC CRL-3216细胞,HEK293T细胞购买价格1800元,人体肾脏细胞系培养基,细胞形态图片，培养条件，冻存条件请见网页，本细胞可以用于转染 ...
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#24SARS-CoV-2 mRNA vaccine design enabled by prototype ...
HEK293T /17 (ATCC CRL-11268), Vero E6 (ATCC), Huh7.5 cells (provided by D. R. Taylor, US Food and Drug Administration) and ACE2-expressing ...
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#25NR-52511 - BEI Resources
Do NOT reference BEI Resources or ATCC Catalog Numbers in the Material Description fields found on the permits and/or forms.
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#26T Antigen Coding Region
HEK293 cell line, lentiviral vector titers obtained using HEK293T ... HEK293T cells (CRL-3216, ATCC, Manassas, VA, USA) and.
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#27Cell Banking of HEK293T cell line for clinical-grade lentiviral ...
HEK293T cell banks were used to manufacture clinical-grade ... MCB and WCB of the HEK293T cell line (CRL-11,268; ATCC, Mansassa, VA, USA; ...
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#28Transient transfection protocol for HEK293T cells - EuroMAbNet
Cell line: HEK293T - Human embryonic kidney cell line (293T CRL-3216, from the ATCC cell bank, USA or 293T ACC 635 from the DSMZ ...
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#29Using HEK293T Expression System to Study Photoactive ...
HEK293T Cell Culture. HEK293T (ATCC® CRL-11268 TM ) cells were maintained in DMEM (Thermo, 10569-044) supplemented with 10 ...
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#30(ACS-4500) HEK293T/17 SF; Suspension Cells - ATCC - CiteAb
Experimental Model [ACS-4500] HEK293T/17 SF; Suspension Cells by ATCC.
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#31Amaxa® Cell Line Nucleofector® Kit V - the Lonza Picturepark
Average transfection efficiency of HEK-293 cells. HEK-293 cells. (ATCC® CRL-1573™) were transfected with program Q-001 and 5 µg of a plasmid encoding ...
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#32HEK-293T > BRIC
HEK293T cell을 컬쳐할라고 하는데요,, ATCC에 보면 MEDIA를 MEM을 사용하라고 하고, 어디보면 DMEM을 사용하라고 하는데, 어떤것을 사용하는 것이 좋을까요? 그리고 코팅 ...
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#33NGVB - Reagent Repository View
Long Description, HEK293T cells are a human cell line that can be transfected with plasmid DNA ... Depositor, Cells were originally obtained from the ATCC.
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#34The Scattered Twelve Tribes of HEK293 - Biomedical and ...
HEK293T – HEK293T is a HEK293 variant that expresses a temperature-sensitive allele of the SV40 T antigen. This enables the amplification of ...
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#35Brasil - Serum-Free Suspension Adaptation of HEK-293T Cells
Cell culture. HEK-293T cell line (ATCC CRL-3216™) was cultured in T-flasks (25cm²) using Dulbecco's Modified Eagle's Medium (DMEM) ...
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#36Deciphering the Ets-1/2-mediated transcriptional regulation of ...
line HEK293T (ATCC® CRL-11268™) were cultured in DMEM and IMDM media ... Total RNA was isolated from ECV-304, HEK293T and Blood Outgrowth ...
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#37Image-Based Annotation of Chemogenomic Libraries ... - MDPI
human cell lines: human embryonic kidney cells (HEK293T), ... HEK293T (ATCC® CRL-1573™) and U2OS (ATCC®HTB-96™) were cultured in DMEM.
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#38A robust platform for expansion and genome editing of primary ...
The embryonic kidney cell line HEK293T (ATCC), the breast cancer cell ... used for the nucleofection of NK and HEK293T cells, respectively.
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#39Cell Lines Companies - Labome
Even cell lines maintained at ATCC might not be authentic, as in the case of ... Frottin F et al obtained both HEK293T and HeLa cells from ATCC for their ...
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#40Characterization of the ZFX family of transcription factors that ...
and ZNF711 from female HEK293T cells (which naturally lack ZFY) and performed RNA-seq to ... Human kidney HEK293T (ATCC #CRL-3216) and.
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#41HEK293: An Essential Human Cell Line with a Unexpected ...
Both HEK293 and HEK293T represent adherent cell lines that form a stable connection with coated cell culture dishes. This limits their volume to ...
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#42Figures and data in SIRT2 and lysine fatty acylation regulate ...
Dynamics of K-Ras4a in HEK293T cells. ... cell line (human), HEK293T, ATCC, Cat#: CRL-3216, RRID: CVCL_0063. cell line (human), HCT116, ATCC ...
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#43Bought HEK293T cells from ATCC -- passage number question
A few months ago, someone accidentally threw away all of my frozen aliquots of HEK 293T cells (yes, it sucked).
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#44Cancer Cell Line Encyclopedia (CCLE) - Broad Institute Logo
... teams acquired 1000 cell lines directly from the relevant publicly accessible cell line repositories including ATCC (American Type Culture Collection), ...
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#45Using AdenoAssociated Virus for Gene Delivery
We recommend using the LentiX 293T Cell Line or. HEK 293T/17 (ATCC CRL 11268) cells. We have confirmed efficient AAV production for these cell lines. 2. It is ...
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#46DNA vaccine candidate encoding SARS-CoV-2 spike proteins ...
Cell culture and viruses. The African Green Monkey Kidney (Vero, ATCC CCL81), HEK293T (ATCC® CRL-3216TM) were obtained from ATCC (Old Town ...
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#47A functional reporter system to identify and enrich base editing ...
HEK293T (ATCC CRL-3216), MDA-MB-231 (ATCC HTB-26), DLD1 (ATCC CCL-221) and NIH3T3 (ATCC CRL-1658) cell lines were purchased from the ATCC.
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#48HIV Reagent Program
The NIH AIDS Reagent Program has been transitioned to the NIH HIV Reagent Program which is managed by American Type Culture Collection (ATCC).
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#49Expression of tumor suppressor REIC/Dkk-3 by a newly ...
Cells. The embryonic kidney cell line HEK293 [American Type Culture Collection (ATCC); Manassas, VA, USA], embryonic kidney cell line HEK293T ( ...
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#50microRNA Regulation in Health and Disease
Cell Culture The C2C12 myoblasts (ATCC, USA) were used to determine the function of miR-106a-5p during myogenesis. HEK293T (ATCC, USA) was employed for the ...
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#51HEK293 Cell Line Origins, Cytogenetics, and Expression
HEK293 cell lines display some cytogenetic instability. Links. HEK Cell (Wikipedia) · PDX Xenograft Models · Purchase HEK293 (ATCC) · HEK293 RNAi Services · GLP ...
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#52Cell Banking of HEK293T Cell Line for Clinical-Grade ...
the HEK293T cell line (CRL-11268; ATCC, Mansassa, VA, USA; obtained under License Agreement between. The Rockefeller University and Hospital ...
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#53ATCC 细胞293T/17 SF [HEK 293T/17 SF]_价格-厂家 - 丁香通
... 丁香通致力为您提供最优质的ATCC 细胞293T/17 SF [HEK 293T/17 SF]厂商信息。 Derivation HEK293T/17 SF cells are a derivative of the 293T (293tsA1609neo) ...
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#54Interplay of Connexins and Pannexins in Tissue Function and ...
In the case of AD293 and HEK293T cells, it was possible to detect low levels ... (NRK) (ATCC® CRL-6509TM) and human embryonic kidney cells (HEK293T) (ATCC® ...
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#55Cell Biology of Viral Infections - 第 177 頁 - Google 圖書結果
HEK293T ( ATCC , CRL - 3216 ) cells were cultured in Dulbecco's modified Eagle's medium ( DMEM , high glucose , Lonza ) supplemented as above .
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#56ATCC® 汎用株化細胞 | フナコシ
細胞株名 由来 組織 ATCC ® No 包装 価格 293 胎児腎細胞（Embryonic Kidney） 胎児腎臓 CRL‑1573™ 1 ml ¥110,000; キャンペー... 293T 胎児腎細胞（Embryonic Kidney） 胎児腎臓 CRL‑3216™ 1 ml ¥110,000; キャンペー... A549 肺がん（Lung Carcinoma） 肺 CCL‑185™ 1 ml ¥110,000; キャンペー...
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#57Expression and Analysis of Recombinant Ion Channels: From ...
... plasmid replication when using vectors containing the origin of replication from SV40 due to the expression of SV40 Tantigen (HEK293T, ATCC) [67, 68].
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#58Applications of the immunoglobulin Cw fragment (IgCw ...
Human epithelial cervical carcinoma-derived cell line HeLa (ATCC® number: CCL-2™) and human embryonic kidney 293 cell line HEK293T (ATCC® number: CRL-3216™) ...
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#59Transfection Reagents for Nucleic Acid Transfer into ATCC Cells
ATCC cells have been a key component of genetic research applications for decades. ... The ATCC® GeneXPlus Transfection Reagent (ATCC® No. ... HEK293T/17.
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#60Expression of glycosylated human prolactin in HEK293 cells ...
For this work, the adherent HEK293T (ATCC® CRL-11268™) (Sant'Ana et al. 2018) and HEK293F™ suspension cell line (Life Technologies, ...
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#61HEK293s (Thawing, Suspending Cells, Maintaining attached ...
Thawing HEK cells: 1) Add 30mL new media to 50mL Falcon tube. 2) Remove cells from small liquid nitrogen dewar. [Chen Lab section, 1st box on top, ...
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#62AD-293 Cells | Agilent
The AD-293 cell line is derived from the standard HEK293 cell line, with improved cell adherence and plaque formation properties, which simplifies the ...
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#63Protocols < E-MTAB-7154 < Browse < ArrayExpress < EMBL-EBI
HEK293T cells were obtained from ATCC and verified mycoplasma free (GATC Biotech). The HEK293T reporter line was maintained in DMEM with GlutaMax (Gibco).
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#64What is the difference between Hek293 and Hek293T cell lines?
virus production, you also need to check for particular clones of hek293T (usually available from ATCC) expressing special vectors which will ...
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#65HEK293 / GFP stable cells - Gentarget
Transformed from adherence enhanced HEK293 cells by lentiviral system with eGFP gene. eGFP was expressed under CMV promoter without any tags. Cell line also ...
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#66What is the Difference Between HEK293 and HEK293t
HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA. In ...
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#67Doing Good Science: Authenticating Cell Line Identity
Now there is a consensus standard from an ATCC working group that details how to authenticate cell lines for research use.
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#68CRISPR prime editing with ribonucleoprotein complexes in ...
ATCC. HEK293T cells (1.25 × 105 per well) were seeded in 24-well plates and transfected 24 h later with 375 ng of the PE2 plasmid, 125 ng of the pegRNA.
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#69ERAD‐dependent control of the Wnt secretory factor Evi
Cell lines. HEK293T (ATCC, #CRL-3216) and A375 (ATCC, #CRL-1619) cells were cultured in DMEM (Gibco) and ...
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#70Single-cell quantification of a broad RNA spectrum reveals ...
analyze the total RNAome of human primary fibroblasts, HEK293T, ... passaged four times in fibroblast basal medium (ATCC, PCS-201-030) ...
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#71M 5.5 in Japan on 21 Feb 2022 20:52 UTC - GDACS
HEK293T (ATCC Cat# CRL-3216, RRID: CVCL_0063); HeLa (ATCC Cat# CCL-2, RRID: CVCL_0030); L-Wnt-3A cells (ATCC ...(more).
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#72ZFX acts as a transcriptional activator in multiple types of ...
Cell culture. The human kidney HEK293T (ATCC# CRL-3216), colon HCT116 (ATCC# CCL-247), and breast cancer MCF-7 ...
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#73Lenti-X™ 293T Cells for Superior Lentivirus Packaging
The Lenti-X 293T Cell Line demonstrates excellent virus production because it was specifically designed to do so. The line was clonally selected from a HEK 293T ...
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#74TransIT® Lentivirus System - Mirus Bio
Mirus recommends using HEK 293T/17 cells (ATCC Cat. No. CRL-11268) or FreeStyle™ 293-F. Cells (Life Technologies® Cat. No.
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#75293T人胚肾细胞ATCC:CRL-3216 - WHELAB上海盈湾生物永生 ...
英文名称, Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo. 描述, 293细胞株插入SV40 T-抗原 ...
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#76Hek 293 food list As shown recently through in vitro means ...
... 293 Database 293 Cells (CRL-1573) in the ATCC database Transcript of FDA meeting, ... review the The Dharmacon™ HEK293T cell line is shipped on dry ice.
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#77Neutralizing antibody responses elicited by SARS-CoV-2 ...
HEK293T -ACE2 cells were infected with the virus-serum mixture, ... ATCC) were maintained in Dulbecco's Modified Eagles Medium (Gibco, ...
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#78HEK293T Cell Line - Horizon Discovery
The HEK293T cells have been tested for viability and verified to contain no. Mycoplasma contamination. Protocol for culturing HEK293T cells. Components for 10% ...
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#79FarnhamLab_Cell Culture Protocol for T-REx HEK293 cells (1)
T-REx HEK293 (Invitrogen Catalog # R710-07); derived from HEK293 cells (ATCC CRL-1573):. Human Embryonic kidney cells; female embryonic kidney cells ...
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#80Lentiviral standards to determine the sensitivity of assays that ...
... human cells (American Type Culture Collection—ATCC, Manassas, ... A subculture of HEK293T human embryonic kidney cells was derived at ...
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#81Vumc C2hr - biowelt direkt
ATCC is a nonprofit organization that collects, stores, and distributes ... HR and NHEJ repair in HEK293T cells were measured as described previously using ...
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#82Vumc C2hr - fuersorgetag-consozial.de
ATCC is a nonprofit organization that collects, stores, and distributes ... HR and NHEJ repair in HEK293T cells were measured as described previously using ...
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#83HEK Cell Splitting and Maintenance | Şen Lab
Human embryonic kidney cells 293 (HEK-293) and 293T cells (those that contain SV40 Large T-antigen) show a reliable growth and have a propensity for ...
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#84Vumc C2hr
ATCC is a nonprofit organization that collects, stores, and distributes ... HR and NHEJ repair in HEK293T cells were measured as described previously using ...
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#85Human Colorectal Adenocarcinoma Cell Line (ATCC HTB-38)
Description HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs ...
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#86Cell Line - BCRC Strain Collection Catalog & Shopping Cart
Description: this is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA; the cells express the ...
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#87Mutant KRAS Cell Lines - National Cancer Institute
Cancer Cell Lines with Mutant KRAS Genes ; HPAF-II, pancreas, G12D, 1, ATCC ; HUP-T4, pancreas, G12V, 1, Sigma.
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#88Human ACE2 Stable Cell Line - HEK293T (CSC-RO0641)
This cell line is engineered to stably overexpressing human angiotensin I converting enzyme 2 (ACE2). HEK293T is derived from HEK293 and is commonly used in ...
hek293t 在 Facebook 的精選貼文
宗教牌👍🏻
（利申：我日日用HEK293T和人類胚胎幹細胞，大哥哥。）