【透過mRNA檢測，輔助判斷停針】流浪貓小虎收到科學園的檢測報告：Negative。小虎可以停藥了。這份診斷有多準確呢？我特意翻查了相關論文：http://bit.ly/2NSjnMc （家長如果要做mRNA檢測，建議停針後兩週才做，不要在打針療程中做，後面再解釋）

FIP貓傳染性腹膜炎向來是難以確診的，學術界公認黃金標準是抽取內臟組織作IHC化驗，才能接近100%準確。還有其他辦法嗎？荷蘭Utrecht University的病毒學研究所，於2004年論證了『透過檢測冠狀病毒的mRNA』，以輔助診斷接受441治療的貓貓是否還帶有冠狀病毒，準確率92.5%。也就是說，小虎有92.5%機會擺脫了FCoV/FIP。《GS441治療為何會復發？九大原因》https://bit.ly/36ygMAp

#注意坊間獸醫使用的快速測試testkit是不能直接檢測FIPV的！它們只能檢測FCoV，而50貓可能有FCoV。不要用快速測試test kit來確診FIP或做停針判斷！

停藥絕對不只是依據這份mRNA報告，必須還要血檢判斷，以及獸醫的臨床診斷，三者結合。我會審慎樂觀去看待『康復』，小心經年累月繼續觀察，繼續供給營養品，不敢鬆懈。跟獸醫商量之後，為了鞏固康復，小虎雖不需要加入免疫療法，但會口服441藥丸到14週才正式停藥。屆時應該是經歷了46日打針，52日口服，合共抗戰98日。

小虎6月5日病徵和確診：http://bit.ly/2HQMYlo
第一周441治療救命記錄：http://bit.ly/2HQxKg5

【什麼是mRNA？】
它是合成蛋白質必須的一種RNA，它承載了DNA的遺傳信息，等於合成蛋白質的說明書、範本，它承載的訊息指導其他遺傳密碼如何正確地合成各種蛋白質。沒有它，便不能合成蛋白質，包括病毒在內。所以，mRNA叫做messenger RNA（信使RNA、信使核糖核酸），即是郵差叔叔。

貓冠狀病毒FCoV的複製，必須由FCoV相關的mRNA指導完成。如果體內沒有檢測到這種mRNA，即是體內沒有病毒複製活動。mRNA在遺傳密碼中的意義和地位，可參考：http://bit.ly/2MXH2ed、http://bit.ly/2MVqFik

【荷蘭實驗mRNA檢測FIP過程簡述】
1）98隻貓樣本，解剖屍體檢驗了內臟組織，其中81隻100%確認FIP，17隻死於其他疾病
2）從這些貓的全血中，分離出會被FIP病毒攻擊的peripheral blood mononuclear cells （外周單核血細胞。另一種最常被攻擊的是巨噬細胞）

#研究人員這個作法非常關鍵而且聰明，因為FECV是不會侵入單核細胞的，但FIP可以。因此，在單核細胞檢測到FCoV，那就是FIPV病毒的訊號了。這裡有些複雜，請先了解FECV和FIPV的分別：http://bit.ly/38TFhWE

3）使用RT-PCR逆轉錄方法，檢視其基因序列，放大成基因圖譜（PCR技術發明者Kary Banks Mullis獲得1993年諾貝爾化學獎）
4）使用顯微技術，檢測其中FCoV冠狀病毒家族相關的mRNA

【實驗結果】
1）81隻FIP貓中，成功在75隻單核血細胞樣本發現FCoV相關的mRNA。所以這種檢測手段的『準確率應是92.5%』
2）在17隻非FIP貓中，完全檢測不出FCoV相關的mRNA，100% (如果你用來確診FIP，其實不太適合。不要以為陰性就一定不是FIPV。太複雜，先不說了）
3）論文中同時引述多個研究結果，論證了為何檢測FCoV病毒的mRNA跟FIP診斷有密切關係。研究人員從Dr Pendersen在1987年發現了豬的冠狀病毒出現在貓的糞便中說起⋯

#如果貓有FIP並已經開始注射GS441，中途去檢測mRNA的話，檢測到陰性的機會很高。因為GS441的責任就是中止病毒繁殖，所以會檢測不到mRNA反應。所以，如果要做mRNA測試，建議停針後兩週才做。

看完論文，更清楚知道領導『#GS441524 病毒抑製劑療法』的Dr Pedersen，在幾十年來對FIP研究的領導地位和偉大貢獻（如果你發現幾乎所有FIP相關論文的引用資料，都不可能避開他的名字，就知道他的獸醫學術界地位在什麼層次了）。有賴他和UC Davis School of Veterinary Medicine 團隊，以及其他病毒學家苦苦研究幾十年，一小步一小步的論證，小虎此刻才有了生存的機會。這是一場真正的時代革命。

逃犯條例引爆69大遊行，流浪貓小虎當日已打了第一針441。9月4日，林鄭月娥宣布撤回逃犯條例。小虎同日收到科學院的檢測報告：Negative。小虎可以停藥了，很特別的日子。

大家的貓貓如果中招有了FIP，可以加入這兩個群組求救和查詢。裡面有經驗豐富的管理員。小虎當初不是及時查詢，早已經死了：
【香港：FIP 腹膜炎 (最新資訊) 治療群組】
https://www.facebook.com/groups/fipcatvirushk/
【海外：FIP Warriors群組】
https://www.facebook.com/groups/158363205096283/

--------------------------------
腹膜炎病毒如何突變而來？貓需要隔離嗎：http://bit.ly/38TFhWE
認識GS441是什麼：http://bit.ly/38cqWUO
口服 vs 打針，打針會更有效：http://bit.ly/2ZWpwhr
GS441治療為何會復發？九大原因：https://bit.ly/36ygMAp
小虎的營養品清單：http://bit.ly/2NNkbBm
恐怖的fip腹膜炎腦神經病變：http://bit.ly/2Qt7uNj
小虎6月5日確診病徵記錄：http://bit.ly/2HQMYlo
第一周441治療救命記錄：http://bit.ly/2HQxKg5
透過mRNA檢測輔助停針：http://bit.ly/2MGg34f
甚麼是 #FIP：http://bit.ly/2NOtEc6（中譯：http://bit.ly/2NLvJFO）
#GS441524 病毒抑制劑研究報告：http://bit.ly/2XEemN4、http://bit.ly/2JzCsib（中文版：http://bit.ly/2JZLaGU）
治療嚴重的眼部腦部病變：http://bit.ly/2SosZAA、http://bit.ly/2Zb71jW
腹膜炎的一切知識：http://bit.ly/2HPPKab
看懂驗血報告：http://bit.ly/2HgXulr（中文：http://bit.ly/2ZmUdHp）
#cat #FIP #傳染性腹膜炎 #kitten #貓傳腹 #GS441

希望大家可以幫吓可仁啦😢 我希望個個小朋友都可以健康快樂咁成長，可以一齊去公園玩

【最新消息：募捐行動已於2018年12月3日結束】中大校友慈善基金感謝各界慷慨支持，募捐行動已結束。所得善款將支持張可仁及患上相類疾病需免疫治療的其他6位病童。

詳情請參閱中大校友慈善基金網頁：https://www.facebook.com/cuhkacf.org/posts/589827338133781

-------------------------------------------------------------------------------------
(2018/11/22)

中大醫學院向大家作出緊急呼籲，支持我們2000年的醫科畢業生張俊傑醫生，拯救他只有17個月大的兒子張可仁的性命。

可仁早於出生第4天時已被診斷罹患一種罕有心臟病「主動脈狹窄」(Coarctation of Aorta)，需要接受緊急手術。極之不幸的是，可仁最近再被確診患上「第四期神經母腫瘤」(Stage IV Neuroblastoma)，目前可仁的頭骨至腰椎和盆骨均受到癌細胞擴散，脊髓神經線受到癌細胞壓住，痛楚非常，並有癱瘓的危機，癌細胞更正轉移至骨髓。

可仁現時正住在威爾斯親王醫院兒童癌症中心隔離病房，極容易受到感染。他已開始接受化療，而治療方案將包括化療、手術、放射治療、骨髓移植、免疫治療，治療時間約一年至一年半。他完成化療、手術和放射治療後，會進行骨髓移植，之後需要免疫療法，這可大大提高存活率，由20%升至50%。

然而，免疫治療需要病人自費，所需費用高達港幣200萬元。張醫生另一兒子患有自閉症，太太辭掉小學主任一職，全職照顧兩位小朋友，並要照顧父母親，經濟上未能承擔高昂的醫療費用。

中大醫學院2000年畢業同學會發起是次籌款，目的包括：
1. 為可仁進行骨髓移植後的免疫治療；
2. 引發社會人士及政府對兒童罕有癌症治療的關注。

查詢：
香港中文大學校友慈善基金
電郵：info@cuhkacf.org
電話：2180 4316

-------------------------------------------------------------------------------------

【Fundraising Campaign Completed on 3 December 2018】According to CUHK Alumni Charity Foundation, the fundraising campaign for Mark CHEUNG Ho Yan has completed. The fund raised will be used to support the immunotherapy of Mark and 6 other patients who are suffered from the same disease. Thank you for all your kind-hearted support and generosity.

-------------------------------------------------------------------------------------

(2018/11/22)

This is an urgent appeal for your generosity to save the life of the 17-month-old son of Dr. CHEUNG Chun Kit, our medical alumnus of Class 2000.

Dr. Cheung Chun Kit is a CUHK medical graduate of Year 2000. His younger son, Cheung Ho Yan Mark, has recently been diagnosed to have Stage IV Neuroblastoma in November 2018, and is in an emergency need of donation to provide the necessary funding to support the life-saving yet expensive immunotherapy.

Mark was presented to his paediatrician for fever and cough in late October 2018. His cough resolved with treatment but his fever never completely went away. Two days prior to his diagnosis, Mark was more clingy than usual and did not like to walk. On a video clip, Mark was noted to have foot drop, a very significant red flag which may indicate a compression on the spinal cord. Emergency scans were immediately performed that night, and Mark was found to have a large tumour on the left side of his abdomen. The aggressive tumour had already spread silently to the back of his skull, his spine, his hips and had compressed his nerves which control his lower limbs, resulting in pain on standing and difficulty in walking. Mark is facing an imminent risk of paralysis.

Mark is diagnosed to have a rare childhood cancer which is called Stage IV neuroblastoma, a cancer which typically presents at late stage because it arises deep in the abdomen. To cure his aggressive cancer, Mark needs to survive through rounds of intensive chemotherapy, radiotherapy, surgery, bone marrow transplant, and finally, immunotherapy. The whole treatment duration lasts 1.5 to 2 years. To completely eradicate all cancer cells, immunotherapy will be needed to boost Mark’s survival from 20% to 50%. However, immunotherapy is a self-financed treatment in the public hospital and the treatment costs could be up to 2 million.

Such close encounter with death is not the first time for Mark. At the first week of life, Mark was diagnosed to have a rare heart problem called “Coarctation of Aorta”. Mark had received life-saving surgery to reestablish the blood flow across his aorta, the major blood vessel that allows blood to go from the heart to the brain and the rest of the body. In a report published in 2002, only 67 cases with dual heart condition and neuroblastoma were reported, giving an odd of 1 in 100 million for a child to be hit by these two rare diseases. Scientists have not yet completely understood the mechanisms that may cause the two conditions to appear in a single child.

It never rains but pours that Mark’s elder brother, now 5 years old, was diagnosed to have autism at around age 2. To enable the elder brother to have intensive training to improve his outcome and development, Mark’s mommy quit her job as a primary school teacher since the diagnosis of autism was made for his elder brother. Dr. Cheung, who is a medical doctor for the elderly, became the sole bread-winner of his family.

CUHK Medical Graduates of Year 2000 have initiated a fund-raising campaign for Mark and his family with the following two objectives:

1. To appeal for donation to support the costs of immunotherapy for Mark
2. To increase the public awareness on the grave needs of children with cancers

Enquiries:
CUHK Alumni Charity Foundation Limited
Email: info@cuhkacf.org
Telephone: 2180 4316

🙏🏻🙏🏻🙏🏻

【最新消息：募捐行動已於2018年12月3日結束】中大校友慈善基金感謝各界慷慨支持，募捐行動已結束。所得善款將支持張可仁及患上相類疾病需免疫治療的其他6位病童。

詳情請參閱中大校友慈善基金網頁：https://www.facebook.com/cuhkacf.org/posts/589827338133781

-------------------------------------------------------------------------------------
(2018/11/22)

中大醫學院向大家作出緊急呼籲，支持我們2000年的醫科畢業生張俊傑醫生，拯救他只有17個月大的兒子張可仁的性命。

可仁早於出生第4天時已被診斷罹患一種罕有心臟病「主動脈狹窄」(Coarctation of Aorta)，需要接受緊急手術。極之不幸的是，可仁最近再被確診患上「第四期神經母腫瘤」(Stage IV Neuroblastoma)，目前可仁的頭骨至腰椎和盆骨均受到癌細胞擴散，脊髓神經線受到癌細胞壓住，痛楚非常，並有癱瘓的危機，癌細胞更正轉移至骨髓。

可仁現時正住在威爾斯親王醫院兒童癌症中心隔離病房，極容易受到感染。他已開始接受化療，而治療方案將包括化療、手術、放射治療、骨髓移植、免疫治療，治療時間約一年至一年半。他完成化療、手術和放射治療後，會進行骨髓移植，之後需要免疫療法，這可大大提高存活率，由20%升至50%。

然而，免疫治療需要病人自費，所需費用高達港幣200萬元。張醫生另一兒子患有自閉症，太太辭掉小學主任一職，全職照顧兩位小朋友，並要照顧父母親，經濟上未能承擔高昂的醫療費用。

中大醫學院2000年畢業同學會發起是次籌款，目的包括：
1. 為可仁進行骨髓移植後的免疫治療；
2. 引發社會人士及政府對兒童罕有癌症治療的關注。

查詢：
香港中文大學校友慈善基金
電郵：info@cuhkacf.org
電話：2180 4316

-------------------------------------------------------------------------------------

【Fundraising Campaign Completed on 3 December 2018】According to CUHK Alumni Charity Foundation, the fundraising campaign for Mark CHEUNG Ho Yan has completed. The fund raised will be used to support the immunotherapy of Mark and 6 other patients who are suffered from the same disease. Thank you for all your kind-hearted support and generosity.

-------------------------------------------------------------------------------------

(2018/11/22)

This is an urgent appeal for your generosity to save the life of the 17-month-old son of Dr. CHEUNG Chun Kit, our medical alumnus of Class 2000.

Dr. Cheung Chun Kit is a CUHK medical graduate of Year 2000. His younger son, Cheung Ho Yan Mark, has recently been diagnosed to have Stage IV Neuroblastoma in November 2018, and is in an emergency need of donation to provide the necessary funding to support the life-saving yet expensive immunotherapy.

Mark was presented to his paediatrician for fever and cough in late October 2018. His cough resolved with treatment but his fever never completely went away. Two days prior to his diagnosis, Mark was more clingy than usual and did not like to walk. On a video clip, Mark was noted to have foot drop, a very significant red flag which may indicate a compression on the spinal cord. Emergency scans were immediately performed that night, and Mark was found to have a large tumour on the left side of his abdomen. The aggressive tumour had already spread silently to the back of his skull, his spine, his hips and had compressed his nerves which control his lower limbs, resulting in pain on standing and difficulty in walking. Mark is facing an imminent risk of paralysis.

Mark is diagnosed to have a rare childhood cancer which is called Stage IV neuroblastoma, a cancer which typically presents at late stage because it arises deep in the abdomen. To cure his aggressive cancer, Mark needs to survive through rounds of intensive chemotherapy, radiotherapy, surgery, bone marrow transplant, and finally, immunotherapy. The whole treatment duration lasts 1.5 to 2 years. To completely eradicate all cancer cells, immunotherapy will be needed to boost Mark’s survival from 20% to 50%. However, immunotherapy is a self-financed treatment in the public hospital and the treatment costs could be up to 2 million.

Such close encounter with death is not the first time for Mark. At the first week of life, Mark was diagnosed to have a rare heart problem called “Coarctation of Aorta”. Mark had received life-saving surgery to reestablish the blood flow across his aorta, the major blood vessel that allows blood to go from the heart to the brain and the rest of the body. In a report published in 2002, only 67 cases with dual heart condition and neuroblastoma were reported, giving an odd of 1 in 100 million for a child to be hit by these two rare diseases. Scientists have not yet completely understood the mechanisms that may cause the two conditions to appear in a single child.

It never rains but pours that Mark’s elder brother, now 5 years old, was diagnosed to have autism at around age 2. To enable the elder brother to have intensive training to improve his outcome and development, Mark’s mommy quit her job as a primary school teacher since the diagnosis of autism was made for his elder brother. Dr. Cheung, who is a medical doctor for the elderly, became the sole bread-winner of his family.

CUHK Medical Graduates of Year 2000 have initiated a fund-raising campaign for Mark and his family with the following two objectives:

1. To appeal for donation to support the costs of immunotherapy for Mark
2. To increase the public awareness on the grave needs of children with cancers

Enquiries:
CUHK Alumni Charity Foundation Limited
Email: info@cuhkacf.org
Telephone: 2180 4316