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[爆卦]Exonuclease NEB是什麼？優點缺點精華區懶人包
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#1Properties of Exonucleases and Non-specific Endonucleases
Enzyme Polarity linear linear 3' ext linear blunt Partial Digestion to gen... Exonuclease I (E. coli) 3' → 5' + — 15 — 5 No Thermolabile Exonuclease I 3' → 5' + — 15 — 5 No Exonuclease T 3' → 5' + — 7 — 5 No
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#2Exonuclease I (E. coli) | NEB
DNA specific exonuclease; Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Exonuclease I is ideal for:.
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#3Exonucleases and Non-specific Endonucleases Products | NEB
NEB offers a number of endonucleases and exonucleases that cleave DNA. Choose Product: Exonucleases and Non-specific Endonucleases.
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#4T5 Exonuclease | NEB
T5 Exonuclease. cloned at NEB · recombinant · NEB4 · 37 · No · Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease · Initiates at the 5' ...
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#5Exonuclease T | NEB
Exonuclease T is a ssRNA or ssDNA specific nuclease that requires a free 3' end to remove nucleotides 3' to 5' and is able to generate blunt ends.
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#6Exonucleases and Non-specific Endonucleases | NEB
NEB offers a comprehensive selection of exonucleases and non-specific endonucleases for the cleavage of DNA, all of which have been optimized for robust ...
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#7Lambda Exonuclease | NEB
Lambda Exonuclease. cloned at NEB · recombinant · NEBU · 37 · 75 Heat · DNA specific exonuclease · Catalyzes the removal of nucleotides from linear or nicked ...
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#8Protocol for Lambda Exonuclease (NEB #M0262)
Lambda Exonuclease efficiently degrades 5' phosphorylated linear dsDNA from 5' to 3' direction, leaving supercoiled dsDNA intact.
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#9Exonuclease I (20 U/µL) - Thermo Fisher
Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise ...
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#10Exonuclease I - New England Biolabs GmbH
Exonuclease I, Article Number: M0293@ ... Download the NEB App. © Copyright 2020 New England Biolabs. All Rights Reserved ...
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#11GSM3132064 - GEO Accession viewer
... NEB M0373S, 0.83 µl 10× T4 RNA ligase buffer, NEB, ... 5 U Lambda exonuclease, NEB, 5 U 5' deadenylase, NEB) and the reaction was ...
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#12Exonuclease I (E. coli) (M0293) - New England Biolabs Japan
ニュー・イングランド・バイオラボ －NEBは制限酵素、エンドヌクレアーゼ、リコンビナント酵素、PCR用試薬、発現システム、マーカー、コンピテントセル、RNA研究用 ...
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#13T5 exonuclease-dependent assembly offers a low-cost ...
Self-prepared Gibson, 3.03, 0.08 U T5 exonuclease (NEB), 0.5 U Phusion DNA polymerase (NEB) and 80 U Taq DNA ligase (NEB), (15).
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#14s41467-018-05265-7.pdf - Nature
exonuclease activity of T4 DNA polymerase (Fig. 1c). Over- ... exonuclease (NEB), 2 × NEBuffer 2, 0.1% Triton-X 100, and 5% DMSO was.
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#16Plasmid-Safe™ ATP-Dependent DNase - Lucigen
Baseline-ZERO™ DNase · Exonuclease I, E. coli · Exonuclease III, E. coli · Exonuclease VII · Plasmid-Safe™ ATP-Dependent DNase · Rec J Exonuclease ...
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#17Gel analysis of the effects of lambda exonuclease treatment ...
Adapter (20 fmol) was ligated to 150 ng of BbvI-digested genomic DNA in a volume of 10 m l for 30 min at 25 ° C in the presence of 1 Q T4 DNA ligase buffer (NEB) ...
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#18GSM4696202 - GEO Accession viewer
... DNA was subjected to λ exonuclease digestion by incubating the beads with 50 μL of λ exonuclease reaction buffer (NEB) containing 0.1% ...
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#19A novel method to precisely quantify hepatitis B virus ...
Total DNA was extracted and ~1 μg was digested in a 20 μL reaction volume at 37 °C with either 10U T5 Exonuclease (NEB) for 30min or a combination of 20U ...
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#20Lambda Exonuclease Reaction Buffer B0262 material safety ...
Many NEB products are classified as mixtures which contain hazardous ingredients in concentrations (less than 1%). The quantity of hazardous chemical ...
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#21New England Biolabs, Inc. T7 Exonuclease - Fisher Scientific
Shop New England Biolabs, Inc. T7 Exonuclease - 1000 units at Fishersci.com. ... FREE FREIGHT on NEB orders greater than $350.00! Catalog No. 50-811-689.
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#22Exonucleases - MedStore
next-generation sequencing (NGS), PCR and gene synthesis. NEB#. PRODUCT. SIZE. LIST PRICE. DISCOUNTED PRICE. M0206S. Exonuclease III (E. coli). 5,000 units.
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#23TCEFS-NEB 2020 full price list.xlsx
Exonuclease T - 250 units. $73. $61. M0267E. Taq DNA Polymerase with ThermoPol Buffer - 20,000 units. $1,224. $948. 2020 TCEFS-NEB price ...
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#24Activities of human exonuclease 1 that promote cleavage of ...
Eukaryotic exonuclease 1 functions in replication, recombination, ... 10 mM DTT, 140 nM plasmid DNA, 50 units of T7 RNA polymerase (NEB), ...
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#25Structural and Biochemical Studies of a Moderately ... - PLOS
A novel exonuclease, designated as MszExo I, was cloned from Methylocaldum szegediense, a moderately thermophilic ... E-mail: [email protected].
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#26Polymerases from NEB
Currently,. NEB supplies DNA polymerases differing in properties such as temperature preference, proofreading exonuclease activity, processivity ...
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#27Real-time measurements of DNA exonucleases - PE Polska
The enzymatic activities of T5 exonuclease and. Lambda exonuclease (NEB), both highly efficient. 5'→3' exonucleases, were investigated. A standard switchSENSE ...
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#28Purification and Sequencing of Large Circular DNA from ...
This step uses qPCR of the human beta globin gene, HBB, to assess the fold-reduction of linear chromosomal DNA after exonuclease digestion and ...
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#29NEBNEB牌M0206L,Exonuclease III (E. coli ... - 吉泰生物商城
NEB 牌M0206L,Exonuclease III (E. coli) ,25,000 units 核酸外切酶III. 商品编号：01.NEB.M0206L; 货号：01.NEB.M0206L; 品牌：NEB; 包装大小： L（大包装）.
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#30Plasmids 101: Common Lab E. coli Strains - Addgene Blog
recBCD, Exonuclease V activity abolished ... NEB Stable, For cloning into and storage of lentiviral and retroviral vectors or cloning or ...
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#31T7外切核酸酶如何与λ外核酸酶（NEB＃M0262）不同？|鼻
T7 Exonuclease has even less activity on single stranded DNA than Lambda Exonuclease, acts at nicks unlike Lambda Exonuclease, ...
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#32نوكلياز داخلي - ويكيبيديا
"Computer Aided Identification of a Potential 5′-3′ Exonuclease Gene Encoded by Escherichia coli". Journal of Theoretical Biology.
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#33Exonuclease I, Nucleases - Jena Bioscience
Exonuclease I · removal of residual ssDNA, including oligonucleotides, from reaction mictures · does not degrade double-stranded DNA or RNA · pequires magnesium ...
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#34Oppd payment extension
Headquartered in Omaha, Neb. ... is 6-Carboxyfluorescein, dSpacer is an exonuclease site, and C3 Spacers is a polymerase extension blocking site Zhao et al.
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#35Q5 polymerase pcr conditions
The NEB Tm Calculator should be used to determine the annealing temperature when using this ... thermostable DNA polymerase with3'-5' exonuclease activity, ...
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#36NEB PCR Cloning Kit from New England Biolabs - Biocompare
You can rely on the quality and value of NEB's cloning reagents, which are available for every step in the cloning workflow. Our latest addition is the NEB ...
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#37Restriction enzyme digestion protocol
Elute DNA from gel clean up with water NEB Restriction Enzymes Restriction ... are converted into single-stranded DNA by lambda exonuclease digestion.
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#38Fermentas enzyme buffer chart
Add 3 μl of the appropriate 10X buffer (recipes are in the NEB catalog), 17 μl (or as needed to increase the volume to ... Exonuclease III Modifying enzyme.
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#39Nonradioactive Analysis of Biomolecules - 第 659 頁 - Google 圖書結果
End repair with mung bean exonuclease 1. Add 3 ul mung bean exonuclease (NEB, 10 units/ml). 2. Incubate exactly 10 min at 30°C, put on ice. 3.
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#40Restriction enzyme activity
Relatively abundant - these are the restriction enzymes found in the NEB catalog etc. ... T4 DNA Polymerase has a 3'→5′ exonuclease activity that will, ...
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#41Minimum dna concentration for sequencing
Purify the DNA prior to phosphorylation (NEB # T1030 ). ... Our method relies on the selective elimination of nuclear DNA using an exonuclease treatment …
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#42Exonuclease I - Enzymatics
Product Description: Exonuclease I cleaves single-stranded. DNA in the 3′→5′ direction, releasing 5′ -mono/di- nucleotides and leaving double-stranded DNA ...
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#43Molecular Evolution, Producing the Biochemical Data, Part B
... or Homemade ExoSAP: combine 5 "l of 20 units/"l exonuclease I (NEB catalog no. M0293L) with 15 "l of 1 unit/"l SAP (U.S. Biochemical Corp., catalog no.
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#44Restriction enzyme digestion protocol
High-efficiency Restriction Enzyme Digestion NEB Applications and Reaction ... are converted into single-stranded DNA by lambda exonuclease digestion. k.
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#45K-Neb Super Mesh Nebulizer (NE-SM3) - The Nest ...
K-NEB Mesh Nebulizer has been developed with pediatricians to be useful and convenient for children. It is a sensitive medical device that ...
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#47實驗試劑/ E. coli Exonuclease III 10 000 U (100 µl) - mylab
實驗試劑/ E. coli Exonuclease III. 型號: M1223-100 規格: 10 000 U (100 µl) 產地: 美國 報價: 8,250 元 運費: 以PDF報價單為準 交期: 期貨，下週到貨
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#48ExS-Pure - Reliable enzymatic PCR clean-up | NimaGen
ExS-Pure is designed for fast and easy PCR cleanup. The reagent kit consists of two recombinant hydrolytic enzymes: Exonuclease-I and Shrimp Alkaline ...
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