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由 C Mayr 著作 · 2019 · 被引用 139 次 — Therefore, 3' UTR-mediated information transfer can regulate protein features that are not encoded in the amino acid sequence.
To systematically elucidate the process of 3′-UTR shortening, we turned to analyze published mRNA sequencing (RNA-seq) data from naive mouse ...
由 Z Huang 著作 · 2017 · 被引用 20 次 — To elucidate the regulatory mechanisms of the 3′-UTR on gene expression, firstly the 3′-UTR sequences must be identified.
3'UTR – The three prime untranslated region is the part of messenger RNA that procedes the translation termination codon. The 3'UTR of mRNA is transcribed ...
UTRdb is a curated database of 5' and 3' untranslated sequences of ... retrieve 5' and 3'UTR variants generated by alternative initiation and termination of ...
Controlling 3 UTR length and sequence facilitates the regulation of the accessibility of functional effectors (RNA binding proteins, ...
Several studies have adapted MPRA to test 3'UTR sequences but to date, none have comprehensively characterized the impact of genetic ...
The 3' untranslated regions (UTRs) of RNA1 and RNA2 are unusually long (~1.5 kb) and share several large stretches of sequence identities.
Therefore, 3′ UTR-mediated information transfer can regulate protein features that are not encoded in the amino acid sequence.
The poly(A) sequence is then recognized and bound by poly(A) binding protein ... hnRNP L binds to CA repeats in the 3'UTR of bcl-2 mRNA.
Assembly of a sequence-specific RNA– protein complex on the 3 untranslated region (3 UTR) of human -globin mRNA ( -complex) correlates with mRNA.
Following up on the potential importance of miRNA targets, we examined the effects of 3' UTR sequences containing predicted targets for miRNAs that were ...
The double-stranded cDNA is PCR amplified, purified, and ready to be sequenced. Pros: Measures 3' UTR isoform abundance to detect protein expression differences ...
The three prime untranslated regions (3' UTRs), that are located downstream of protein-coding sequences, play important roles in post-transcriptional regulation ...
sequence motifs within the 30 UTRs that support polyadenylation ... mon variant in the 3'UTR of the GRIK4 glutamate receptor gene affects ...
Plasmid pAG84 3'UTR from Dr. David Bartel's lab contains the insert N5 3'UTR and is published ... 5′ sequencing primer EBV rev primer (GTGGTTTGTCCAAACTCATC) ...
Key words: GSD-Ia, 3'UTR, rs2229611, polymorphism, linkage disequilibrium, ... Interaction of RNA binding proteins to the G6PC1 3'-UTR sequence was ...
3 -UTR length was obtained from References 26–28, 58, 81, 107, 130, and. 139. (b) Sequence conservation shown as PhastCons scores for genes ...
UTR sequence during evolution, together with the recent ... the DNA sequences from genes of different organisms in.
In the present study, we investigated 3′ UTR lengths in a highly curated set of 57,135 mRNA sequences among four well-studied and ...
potential selenocysteine incorporation sequences in the open reading frame and the 3'-UTR to the translation of mRNA.
Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells. Performed at GE Healthcare Biosciences. Ellen Einarsson.
Abstract. The 3' untranslated region (3'UTR) of mRNA contains elements that play regulatory roles in polyadenylation, localization, ...
Seeding sequence mutagenesis services: Seeding sequence of the 3'UTR clones could be mutated to test the specificity of microRNA and its targets'.
-UTR regulatory sequences impact gene expression independently of one another within Hmga2. ... UTR sequences are also affected by 3 UTR size.
miTarget™ 3'UTR miRNA Target Clones in pEZX-MT05 - Gaussia Luciferase & SEAP. 3' UTR sequences were obtained from public domain gene sequence databases and ...
However, the presence of two identical copies of the 3'-UTR sequence of human beta-globin in plasmid DNA, which is then used as a template for in vitro RNA ...
... a frequent somatic mutation in the glyceraldehyde 3‑phosphate dehydrogenase (GADPH) 3'UTR was identified using transcriptome sequencing ...
Therefore, alterations in the 3′ UTR sequence may lead to disruptions in gene regulation. KRAS, which encodes a GTPase signaling protein, plays ...
... of the nuclear retention of mRNA containing in their 3'-UTR inverted repeats of Alu sequences (IRAlu). In this study, we found that, ...
3' UTR sequences were obtained from public domain gene sequence databases and inserted downstream of a secreted Gaussia luciferase (GLuc) reporter gene, ...
An unexpected finding: When 3' UTR sequences of Sox 11 mRNA in embryonic mouse brain (above) were marked in red and coding sequences in ...
Frequent Changes of 3' UTR Sequences in the Genes Expressed During HematopoieticDifferentiation Implicates the Importance of 3' UTR in Regulation of Gene ...
Recombinant plasmids to transfect these cells lines were engineered by insertion of the variant titin 3'UTR 431- and 1047-base pairs sequences into luciferase ...
Paraspeckles, mRNA targets, RNA nuclear retention, RNA binding protein, 3'UTR sequence determinant, HNRNPK. DECLARATIONS. FUNDING.
Systematic analysis of the 3′-UTRs of destabilized transcripts, identifies enrichment in sequence motifs corresponding to microRNA ...
genes, implying that in 3T3 cells, alternative 39 UTR sequences play a surprisingly small regulatory role compared to other mRNA regions.
The 5′ untranslated region (5′UTR) should be non-regulated, short, unstructured, and without AUG codons. The sequence around the start codon should match the ...
... RNA structures in the viral 3' UTR is under different selective pressures in adult mosquitoes. Sequence analysis of DENV RNA populations ...
Choosing the 3'UTR - 3'UTR of gene of interest : Many genes in the germline are regula. ... 3'UTR sequences must also be included downstream of ORF.
mTOR 3'UTR null knock-out mice carry a CRISPR/Cas9-generated deletion in the Mtor 3' UTR sequence that confers axonal localization of the ...
In addition, 50 UTRs can contain sequences that function as binding sites for regulatory proteins. Similarly, 30 UTRs contain numerous binding sites for.
sequences within the splice-deleted region 672 to 1233 of the 3 UTR, which regulate the transcription, mRNA stability, and translation of TCR mRNA.
UTRdb is a curated database of 5' and 3' untranslated sequences of ... to better visualize and retrieve 5' and 3'UTR variants generated by ...
Fragments cloned into the MCS downstream of the reporter gene will become part of a hybrid transcript that contains the luciferase coding sequence fused to ...
SwitchGear Genomics has made an extensively validated set of 3'UTR plasmid ... Genome-wide pre-cloned 3'UTR sequences provide researchers measurable savings ...
How do you find the 3 UTR sequence? In most cases, the sequence between the 5'end and the start codon of the longest ORF will be the 5'UTR.
GO ID: GO:0003730; Aspect: Molecular Function; Description: Binding to a 3' untranslated region of an mRNA molecule. Synonyms: mRNA 3' UTR binding.
Introns have a beginning- and ending-recognition sequence; it is easy to imagine ... The presence of RNA-binding proteins at the 5' or 3' UTR influences the ...
Our 5' and 3' UTR sequences, cap1, and polyA tail are engineered to ... The protein target is automatically converted to an initial optimized mRNA sequence.
Clustal Omega is a multiple sequence alignment program. It produces biologically meaningful multiple sequence alignments of divergent sequences.
Comparative Analysis of Single-Cell RNA Sequencing Methods. ... RNA within single cells and to obtain accurate information about the 3' UTR.
A common approach is to obtain cDNA sequence and use a sequence translation software program (e.g. ExPASy) to find the longest ORF.
The optimised codon sequence encoding the spike glycoprotein antigen ... RNA stability and translational efficiency (5'-cap, 5'-UTR, 3'-UTR, ...
An mRNA molecule is transcribed from the DNA sequence to be later translated into protein. Several parts of the mRNA are not translated into protein, ...
mRNA has some additional sequences that are not translated and are referred as untranslated regions, (UTRs). The UTRs are present at both 5 end (before start ...
3'UTR ... factors Receptor (biochemistry) Membrane proteins Ribosomes Human Isoform Organism Exons Sequencing Mutation Tumorigenesis Myotonin-protein kinase ...
The 5'-UTR-derived sequences were found in the evaluated β-CoV Merbecovirus ... is half way through exon 3 3' UTR starts after STOP codon (still in exon 3).
During gene expression, an mRNA molecule is transcribed from the DNA sequence and is later translated into a protein. Several regions of the mRNA molecule are ...
The HIV-1 3'UTR sequence was obtained from the National Center for Biotechnology Information (NCBI) database with accession number NC_001802 (86319085).
A 109 nt sequence element located in the 3 ' - UTR of BYDV - PAV between ORFs 5 ... in combination with different 3 ' - UTR sequences ( BYDV or plasmid with ...
... a simplified transcript model containing a 5'UTR, coding sequence and 3'UTR. ... Notes 2.6 Sequences, Genomes, and Genes in R / Bioconductor Table 1.
SeqRNA = dna2rna (SeqDNA) converts a DNA sequence to an RNA sequence by ... It is comprised of a 5′ cap, 5'UTR region, coding region, 3'UTR region, ...
The primers sequences were as follows: ... by bioinformatics analysis, which showed a binding site with miR-124 in the 3'-UTR of LNCOC1.
In recent years, the popularization of next-generation high-throughput sequencing technology has greatly enhanced our understanding of cancer biology [4.
(2014), Meer et al. region Structure (2012) tau 91 nt region Primary sequence 3' UTR Rat Aranda-Abreu et al. (1999), Aronov et al. (2001), Behar et al.
The mRNA stability was determined by the 3'UTR sequence, with “unstable” being operationally defined as displaying an mRNA halflife of less than 1 hr and ...
... region (3'UTR) of most messenger RNAs (mRNAs) in the human genome. ... Using single-cell RNA-sequencing based data, we identified two ...
... and ~124-amino-acid paired domains in PAX proteins), or even collections of conserved short sequence motifs. A. 3' UTR Lα1 α2 α3 TM CY B. ~2.2 Mb E 2 C ...
30 Coat protein gene and 3'UTR sequences of isolate arkl5. The 3' UTR starts at the * position (nt = 1069) The CP genes including the 3' UTR regions of two ...
Moving, swapping, rearranging the large scale order of DNA sequences. ... The 5'UTR and 3' UTR mutations especially in the CODING REGION OF AN EXON.
They recognise specific target sequences in the 3'UTR of some transcripts, mediating their silencing (Fabian et al., 2010) by inducing their degradation or ...
... a sequence directly repeated at bothends of a defined sequence , of the sort ... .coding sequences ( CDS , exon ) and 3'untranslated region ( 3'UTR ) ...
TABLE 2 | Overlapping bovine miRNAs at the 3 UTR, 5 UTR and CDS. ... results show that miRNAs are relatively abundant in the coding sequence (CDS) region, ...
In contrast, the 3'-UTR was found to act as a potent mRNA instability determinant. The most highly conserved sequence in the 3'UTRs of SAUR transcripts is ...
... it is called the 5' UTR (or leader sequence), or if it is found on the 3' ... it is called the 3' UTR (or trailer sequence)UTR Pro Tennis ...
Consider the messenger RNA. The blue sequence is commonly called the untranslated region (UTR) and finishes right before the start codon, shown in red.
... next-generation sequencing (NGS) data analysis pipeline systems, ... Xing # (2021) Analysis of DNA variants in miRNAs and miRNA 3'UTR binding sites in ...
... next-generation sequencing (NGS) data analysis pipeline systems, ... Xing # (2021) Analysis of DNA variants in miRNAs and miRNA 3'UTR binding sites in ...
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