Can any of the Applied Biosystems Real-Time PCR Systems detect one copy? All Applied Biosystems instrument systems are qualified to detect a two-fold change. Scientifically speaking, there is a statistical calculation that predicts the probability of detecting one molecule in any given sample called the Poisson Distribution. The Poisson Distribution predicts that one molecule will be detected 66% of the time. This is assuming that the assay has been optimized to the fullest capacity and that there are no aliquoting or pipetting errors involved. Please refer to the following paper concerning one-copy detection. Lockey, C., E. Otto, and Z. Long. "Real-time fluorescence detection of a single DNA molecule." BioTechniques 24 (1998): 744-746. Each assay designed on one of our platforms needs to be optimized to determine the Linear Dynamic Range of the assay. An Applied Biosystems Sequence Detection System (Real-Time PCR instrument) may be able to detect one copy in a fully optimized assay. As stated above, one copy can only be detected 66% of the time in... 更多資訊 Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)? It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments: • 7900HT Fast Real-Time PCR System • 7500/7500 Fast Real-Time PCR System • StepOne/StepOne Plus Real-Time PCR System • ViiA 7 Real Time PCR System • QuantStudio 1 Real-Time PCR System • QuantStudio 3 & 5 Real-Time PCR System • QuantStudio 6 & 7 Flex Real-Time PCR System • QuantStudio 6 & 7 Pro Real-Time PCR Systems • QuantStudio 12K Flex Real-Time PCR system Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x): 1. Open the data file in the Design & Analysis Software (v2.x). 2. Click on Actions. 3. Click on "Standard Curve Analysis Setting". 4. Select "External Standard Curves" and click on "Import". 5. Browse to the proper .csv file and click "Open". 6. Click "Apply".
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