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並前往https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Roche%20NiNTA%20protocol.pdf.pdf

5.2 Protein purification

*The imidazole concentrations of elution buffers 1 and 2 must be optimized for each protein. Reagent. Vendor. Ni-NTA agarose. QIAgen. 1 ml column with luer ...

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