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並前往https://books.google.com.tw/books?id=N7jO0g2aL9wC&pg=PA27&lpg=PA27&dq=fplc+methods&source=bl&ots=ouHyE-8_Kw&sig=ACfU3U3p0VddQoOVHdIZSWkx2Ht_tAJV5Q&hl=zh-TW&sa=X&ved=2ahUKEwig9pTsrJf3AhWQuJUCHeEGD6IQ6AF6BAhFEAM

Calpain Methods and Protocols - 第 27 頁 - Google 圖書結果

The n should be timed so that the FPLC separation can be run immediately mining the protein content. If a delay occurs, hold the supernatant at 4°C.

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