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並前往https://international.neb.com/tools-and-resources/selection-charts/recleavable-blunt-ends

Recleavable Blunt Ends | NEB

Enzyme Ligated to Recleaved by AfeI (AGC/GCT) BsrBI, MspA1I (CMG/CGG) HpaII AfeI (AGC/GCT) BstZ17I AluI AfeI (AGC/GCT) EcoRV SfaNI

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