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並前往http://users.stlcc.edu/departments/fvbio/Bio219_Lab_Manual/Lab%209%20LIGATION.pdf

lab 9 recombinant dna ligation student guide - STLCC.edu

fragment. When ligating an insert fragment of DNA into a plasmid vector, the molar ratio of insert DNA should be 3-4 times that of the plasmid vector.

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