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並前往https://www.bioss.uni-freiburg.de/fileadmin/data/assets/pdf/SOP_Ligations-Troubleshooting.pdf

Ligation Troubleshooting SOP

If you don't have a change in the ladder (everything sfited up), your ligase is a (the?) problem. → Usual ratio Vektor: Insert =1:3. Increase your vector to ...

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