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並前往https://openwetware.org/wiki/IGEM:Hong_Kong_HKUST/Investigations/_Effects_of_vector_to_insert_DNA_ratio_on_ligation_efficiency

Effects of vector to insert DNA ratio on ligation efficiency

Before ligation, a series of standard cloning processes are needed. Four different ratios of the vector and insert (1:0.5, 1:1, 1:3, and 1:10) ...

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