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並前往https://www.owczarzy.net/Tm_for_duplexes-IDT_Tech.pdf

Calculation of Tm for Oligonucleotide Duplexes Calculation of ...

The melting temperature of an oligonucleotide duplex (Tm) refers to the ... probe-target duplex (which is used at IDT in all of our web calculators.

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