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並前往https://support.snapgene.com/hc/en-us/articles/360054394671-Can-I-configure-SnapGene-to-show-primer-Tm-values-suitable-for-PCR-polymerases-such-as-Phusion-Phire-and-Q5-

Can I configure SnapGene to show primer Tm values suitable ...

For most PCR polymerases, the optimal annealing temperature for the PCR reaction is about 0–5°C below the Tm for the primers. For some...

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