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並前往http://homepage.ntu.edu.tw/~dhkuo/pCRII-TA.pdf

TA Cloning® Kit Dual Promoter (pCR®II )

Taq polymerase has a nontemplate-dependent activity that adds a single deoxyadenosine (A) to the 3´ ends of PCR products. The linearized vector supplied in this ...

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