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並前往https://med.nyu.edu/research/scientific-cores-shared-resources/sites/default/files/frozen-tissue-preparation-with-sucrose.pdf

Tissue preparation and cryopreservation with sucrose

Fix tissues in fresh (<1wk old) 4% “paraformaldehyde” (e.g. Cat # 15714, Electron Microscopy Sciences for 32% stock) at 4oC (see instructions ...

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