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並前往https://dnatech.genomecenter.ucdavis.edu/faqs/how-should-i-submit-the-barcode-sequence-information-in-which-direction-will-they-be-sequenced/

How should I submit the barcode sequence information? In ...

For barcodes added to Illumina libraries via a PCR step (e.g. Nextera; or also onto TruSeq stub-adapters):. index 1 (i7) is always read as reverse complement of ...

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