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並前往https://international.neb.com/faqs/0001/01/01/can-klenow-fragment-3-8594-5-exo-be-used-to-remove-5-overhangs

Can Klenow Fragment (3'→5' exo-) be used to remove 5' ...

No, DNA polymerases cannot remove 5' overhangs. Use Mung Bean Nuclease (NEB# M0250) to remove 5' overhangs.

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