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並前往https://bmb.natsci.msu.edu/sites/_bmb/assets/File/Sharkey_lab/His-tagged%20Protein%20Purification%20with%20Protease%20Inhibitor.pdf

His-tagged Protein Purification with Protease Inhibitor

Qiagen Lysis buffer (Qproteome cat# 37900) (at least 25 ml) fridge, ... 20 ml Bio-rad column with cap and ring stand to hold Ni-NTA agarose.

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