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並前往https://ocw.mit.edu/courses/7-15-experimental-molecular-genetics-spring-2015/b6d9befecddfc6f51fc98157769dceaa_MIT7_15S15_Primer_design.pdf

General guidelines for primer design - MIT OpenCourseWare

The following considerations should be taken into account when designing primers. 1) Primer sequence. The 3'-end of the primer molecule is especially critical ...

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