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並前往https://www.biosearchtech.com/support/faqs/realtimedesign-rtd-software-qpcr-assay-design/how-is-the-tm-calculated-in-biosearchs-realtimedesign-software

How is the Tm calculated in LGC Biosearch's Technologies ...

Our RealTimeDesign™ (RTD™) uses the SantaLucia "unified" nearest neighbor thermodynamic parameters in the algorithm to calculate melting temperature (or TM) ...

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