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PCR​​Using​​Phusion® High-Fidelity​​DNA​​Polymerase

It​​is​​important​​to​​add​​Phusion​​DNA​​Polymerase​​last​​in​​order​​to​​prevent​​any​​primer degradation​​caused​​by​​the​​3´→​​5´​​exonuclease​​activity.

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