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並前往https://manuals.cellecta.com/line-1-mobile-element-ngs-assay/v1/en/topic/step-5-second-round-of-nested-pcr

4.5. Step 5. 2nd Round of Nested PCR - Cellecta

The second PCR amplification step uses the nested GSP2 Anchor Primer to ensure the specific amplification of the correct 3' L1H-specific ...

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