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並前往https://academic.oup.com/peds/article-pdf/doi/10.1093/protein/gzaa027/34694191/gzaa027.pdf

Engineering sensitivity and specificity of AraC-based ...

PCR conditions followed NEB Tm. Calculator (https://tmcalculator.neb.com/) and vendor's instructions for the polymerases. Zymoclean™ Gel DNA Recovery Kit ...

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