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並前往https://arep.med.harvard.edu/kzhang/cgi-bin/myOligoTm.cgi

Kun's Oligonucleotide Tm calculator

Tm calculator using the Nearest-Neighbor method (NN paramters are based on SantaLucia J Jr. (1998) PNAS, 95:1460-5). Tm is adjusted for MgCl2 and DMSO ...

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