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並前往https://international.neb.com/faqs/2016/11/14/why-do-i-have-multiple-peaks-in-my-melt-curve

FAQ: Why do I have multiple peaks in my melt curve? - NEB

A typical denaturation (melt) curve performed after qPCR cycling with an intercalating dye, will typically give rise to a single distinct peak ...

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