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並前往https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/g/t4-dna-ligase-blue-white-cloning-qualified-protocol.pdf

T4 DNA Ligase Protocol - Promega Corporation

We recommend using a 1:1, 1:3 or 3:1 molar ratio of vector:insert DNA when cloning a fragment into a plasmid vector. These ratios will vary with other types of ...

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