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並前往https://www.affymetrix.com/support/technical/usb/brief_proto/78400B.pdf

USB® Ligate-IT™ Rapid Ligation Kit

(See reverse side for molar ratio calculations). 3. Add 4 μl of 5X Ligate-IT Reaction Buffer and mix by pipet- ting. 4. Add 1 μl Ligate-IT T4 DNA Ligase, ...

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