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並前往https://www.goldbio.com/documents/3886/Ligation%20Protocol%20using%20T4%20DNA%20Ligase.pdf

Ligation Protocol with T4 DNA Ligase-Final - GoldBio

Use a molar ratio of 1:3 vector to insert DNA (see Calculations section). Table 1. Set up of ligation reaction (400 units/µl Ligase).

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