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並前往https://tools.thermofisher.com/content/sfs/manuals/MAN0011906_DNAsert_Ligation_Vector_DNA_UG.pdf

DNA Insert Ligation (sticky-end and blunt-end) into Vector DNA

to 1 hour. Linear vector DNA. 20-100 ng. Insert DNA. 1:1 to 5:1 molar ratio over vector. 10x T4 DNA. Ligase buffer. 2 μL. Thermo Scientific T4 DNA.

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