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並前往https://international.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202

Ligation Protocol with T4 DNA Ligase (M0202) | NEB

For blunt ends or single base overhangs, incubate at 16°C overnight or room temperature for 2 hours (alternatively, high concentration T4 DNA Ligase can be used ...

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