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並前往https://www.readcube.com/articles/10.3389/fmolb.2016.00070

SacB-SacR Gene Cassette As the Negative Selection Marker ...

We generated a mutant A. tumefaciens strain GV2260 (recA-SacB/R) that has the SacB-SacR cassette inserted into the bacterial genome at the recA gene locus.

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