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並前往https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/quantitative-pcr-and-digital-pcr-detection-methods

Quantitative PCR and Digital PCR Detection Methods - Sigma ...

Fluorescent dyes or probes are included in PCR mixes to monitor the change ... more commonly referred to as fluorescence resonance energy transfer (FRET).

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