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並前往http://rpdata.caltech.edu/courses/aph162/2006/Protocols/RocheRapidDNALigation.pdf

Rapid DNA Ligation Kit

Molar ratio. • The molar ratio of vector DNA to insert DNA the standard ligation reaction should be 1:3, e.g. 50 ng linearized, dephosphorylated plasmid vector ...

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