雖然這篇trypsin細胞培養鄉民發文沒有被收入到精華區:在trypsin細胞培養這個話題中,我們另外找到其它相關的精選爆讚文章
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#1細胞繼代培養
Trypsin 為胰蛋白酵素,其作用為分解細胞與瓶壁之附著蛋白, EDTA為chelating agent,其作用為去除Ca2+、Mg2+等離子,trypsin與EDTA二者共同作用,可使附著之細胞自瓶壁脫落 ...
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#2Cell culture | 細胞培養
Trypsin -EDTA 濃度常使用0.05%,視情況可以調整濃度,而時間常為1-10分鐘,視細胞種類而定。新接觸的目標細胞建議每1-2分鐘拿出來,於顯微鏡下觀察,成功 ...
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#3細胞培養- Cell Culture
移去培養基,用PBS 洗滌細胞1-2 次後移去PBS,加入trypsin-EDTA. 溶液,放在37℃作用數分鐘後,輕拍培養瓶使細胞自瓶壁脫落,加入. 含血清之新鮮培養基, ...
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#4常見細胞培養問題與回答
附著性細胞繼代時所使用之trypsin-EDTA 常見的問題: Q1: Trypsin-EDTA 使用濃度: 0.05% trypsin-0.53mM EDTA。 Q2. Trypsin作用的時間愈來愈長: 反復冷凍解凍造成trypsin ...
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#5讓細胞- 健康的秘訣
除此之外,細胞培養最常做的便是繼代,不當. 使用胰蛋白酵素trypsin 會導致細胞培養出現問題。 Trypsin 會分解附著於細胞表面之蛋白質,但暴露於 trypsin 下過久可能造成太 ...
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#6細胞之繼代培養
先將trypsin-EDTA回溫. 至37℃。 • 於無菌操作台內,將. 25T-培養盤內的培養液. 取出至廢液桶。 輔仁大學生命科學系. 貼附性細胞之繼代培養 ...
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#7廢液抽吸與細胞培養| 流程與方法 - 洛科
a. 附著型細胞(adherent cell),又稱貼壁培養、單層培養(monolayer culture) · 去除舊培養基廢液 · 以Phosphate buffered saline (PBS) 洗滌細胞一至二次 · 加入trypsin-EDTA ...
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#8BCRCClassroom - 細胞繼代培養
Trypsin 為胰蛋白酵素,其作用為分解細胞與瓶壁之附著蛋白, EDTA為chelating agent,其作用為去除Ca 2+ 、Mg 2+ 等離子,trypsin與EDTA二者共同作用,可使附著之細胞自瓶壁脫落 ...
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#9胰蛋白酶Trypsin - TAQKEY Science 德怡科技
首頁 / 細胞培養試劑Media and Reagents / 細胞培養試劑Cell Culture Reagents / 胰蛋白酶Trypsin. 篩選. 顯示所有5 個結果. 依熱銷度, 依最新項目排序 ...
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#10HiMedia 溫和型Trypsin 替代試劑-Accutase
HiMedia Trypsin · Liquid · 除Gibco/HyClone以外 · 全球最大cell culture培養基產品生產與代工大廠 · 產品線包含動物、植物與微生物培養基 · 為最齊全的培養基供應大廠 (圖片為 ...
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#11細胞培養
細胞培養. Cell Culture ... 培養液. 抗生素. 細胞分離試劑. 3D 細胞培養. 生長因子/ 抗體. 細胞外基質 ... 可取代trypsin,溫和的分離貼附性的細胞.
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#12產品介紹--細胞培養相關試劑
Cat. No. Product. SC-0123, Enzyme-Free Cell Dissociation Solution. SC-0113, Trypsin Neutralization Solution. SC-0103, Trypsin/EDTA solution ...
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#13Trypsin Cell Dissociation Protocol
Trypsin in Cell Culture. Cell dissociation is the process during cell passaging where cells are detached from the treated surface to create suspensions.
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#14胰蛋白酶<span>Trypsin</span> - 產品資訊
用於VERO;MRC;BHK;CEF;MDCK;SF-9;High Five;S2;HSC;MSC;ESC等細胞培養使用。 產品說明. Sheffield rTrypsin ACF 是一種重組形式的豬胰蛋白酶(porcine trypsin),是一種 ...
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#15TRYPSIN-EDTA SOLUTION 10X*CELL CULTURE TESTED
TRYPSIN -EDTA SOLUTION 10X*CELL CULTURE TESTED. NT$3,200. 品牌:SIGMA 品號:T4174 品名:胰蛋白酉每規格:100ML CAS:9002-07-7. TRYPSIN-EDTA SOLUTION 10X*CELL ...
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#16Trypsin: Cell Culture Products
Trypsin : Cell Culture Products. PRSS (Protease; serine) genes produce three isoforms of pancreatic trypsinogen. Based on their electrophoretic mobilities, ...
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#17ScienCell 細胞培養工具
細胞 是萬物生長的源頭,培養細胞分化生長的過程中,需要給與足夠的營養素,如:醣類、胺基酸、 ... Trypsin Neutralization Solution, 200 ml, 0113-2.
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#18Cell Dissociation and Trypsin for Cell Culture
Compare and select cell dissociation reagents including Gibco trypsin and TrypLE reagent to find the right product for your adherent cell culture.
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#19High-level production of recombinant trypsin in transgenic ...
High-level production of recombinant trypsin in transgenic rice cell culture through utilization of an alternative carbon source and recycling system.
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#20Gibco™ Trypsin-EDTA (0.05%), phenol red
Home · Products · Cell Culture Media, Supplements, and Reagents · Cell Culture Reagents, Antibiotics and Supplements · Dissociation Reagents · Gibco™ Trypsin-EDTA ( ...
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#21CAS-(9002-07-7), Trypsin 1:250 Powder Source
CDH is an ISO certified Trypsin 1:250 Powder Source : Porcine Pancreas Cell Culture Tested1000-1500 BAEE units/mg manufacturer in India, Trypsin 1:250 ...
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#22Trypsin, 100 mg, CAS No. 9002-07-7
Trypsin, 100 mg, ≥2500 USP-U/mg, cryst.⮞A pancreatic serine protease with a specificity for peptide bonds between basic amino acids arginine...
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#23Detection of contaminants in cell cultures, sera and trypsin
The PCR protocols were applied to 88 cell culture samples from eight laboratories. The tests were also used to assess potential contamination in 10 trypsin ...
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#24Trypsin
Generon > · Research · Cell culture · Cell Culture Reagents · Trypsin ...
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#25Trypsin from porcine pancreas, | T4799-25G
Trypsin from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture, 1000-2000 BAEE units/mg solid.
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#26r-Trypsin - Type Standard ACF ex. Porcine 9002-07-7
r-Trypsin - Type Standard ACF ex. Porcine for cell culture, 800U/mg, Endotoxin (BET) 0.05EU/mg ; Storage · -20 °C (Blue/Dry Ice) ; Shelf Life · 60 Months ...
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#27【Abmgood細胞培養】貨號#TM050。 Trypsin-EDTA 0.25 ...
Cell Culture Products 胰蛋白酶(細胞培養)Trypsin-EDTA 0.25%Solution100ml | 500mlTrypsin-EDTA Cat.No.:
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#28細胞治療產品應用專刊
分離人類檢體,單核球,此分離液經後續細胞培養測試細胞存活率(Splenocyte separation: ... Recombinant Trypsin Solution without EDTA.
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#29組織培養組培:發展簡史,套用前景,分類,步驟,培養基配製,滅菌
附著型細胞adherent cell ,又稱貼壁培養、單層培養去除舊培養基廢液以洗滌細胞至二次加入trypsin EDTA溶液,於2~20 分鐘,使細胞可自培養瓶/皿中脫落加入含有血清的 ...
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#30以無血清微載體細胞培養量產新型流行性感冒疫苗
由UK NIBSC 得到的疫苗種株(NIBRG-14)在胚胎蛋及MDCK 細胞培養的效. 價為107~8 ... 使細胞脫落後,需先添加4ml 的Trypsin inhibtor,再加入8ml 新鮮培養基。
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#31Trypsin – Biowest | Your best choice for animal serum
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of “anchorage-dependent” mammalian cells and ...
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#32繼代細胞 - 實驗室廢宅腐女- 痞客邦
前準備:medium、PBS、trypsin這三罐從冰箱拿到37度水浴槽回溫,從培養箱(incubator)中拿出細胞在顯微鏡底下觀察,紀錄滿度。 環境整理.
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#33細胞生物實驗
酵素動力學, 斑馬魚受精卵的發育過程, 血液中血球的分離, 分散式細胞培養 ... 加入1ml的trypsin/EDTA 輕微搖晃並輕敲培養皿底部,經過1-2分鐘確定細胞懸浮之後直接 ...
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#340.25% Trypsin-EDTA, 1X
[活動] 新品上市! 消費滿額享加價購! 藍光系統(BLooK™) · 細胞培養(Cell culture medium) · Antibiotics · Balances Salts solution · Basal ...
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#35產前染色體分析與培養
診斷方式,但傳統的細胞培養及染色體鏡檢仍然為不可取代的磐石。 陳持平. 羊水驗收程序 ... (1) 第一次先用1ml的胰蛋白清洗,吸到原離心管中(因為Trypsin 無法在有培養液.
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#36Sterile Cell Culture Technique 細胞培養無菌技術
00:36 3. Remove culture medium. 00:41 4. Wash cells. 00:49 5. Add trypsin.
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#37细胞传代培养常见问题合集!
去掉原T25培养瓶里面的培养基,T25瓶加3-4 ml PBS洗1-2次;弃PBS,再加1.5 ml的Trypsin-EDTA (1X)消化液消化细胞,显微镜下观察,待细胞变圆,细胞 ...
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#38Trypsin, TPCK Treated 胰蛋白酶(TPCK处理)(组织培养级 ...
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物 ...
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#39Trypsin EDTA Solution A (0.25%), EDTA (0.02%)
Home > · Products > · Cell Culture > · Trypsin and Cell Dissociation > · Trypsin EDTA Solution A (0.25%), EDTA (0.02%).
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#40Trypsin-EDTA胰蛋白酶|TM015 | 太鼎生物科技
Trypsin -EDTA胰蛋白酶|TM050. Trypsin-EDTA為收集吸附型細胞常用之方法。Trypsin為胰蛋白酵素,其作用為分解細胞與瓶壁之附著蛋白 ...
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#41原代細胞培養方法- 細胞培養步驟
加入trypsin EDTA溶液: 方法:37℃或室溫作用數分鐘,於倒立顯微鏡下觀察,當細胞將要分離而 ... 细胞培养分为两大类:贴壁培养adherent culture又称单层培养、悬浮 ...
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#423D培養之小兵立大功的球狀多細胞
Cell morphology: 細胞能夠聚集或者形成球狀多細胞(Multi-cellular spheroid),非2D培養的平面單層細胞。 Cell Stiffness: 細胞所承受的剛性能更接近生物 ...
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#43Trypsin EDTA Solution C (0.05%), EDTA (0.02 ...
作为国内专业化的细胞培养平台,我们提供各类干细胞干细胞,免疫细胞和原代细胞无 ... Trypsin EDTA Solution C (0.05%), EDTA (0.02%), without Phenol Red 胰酶(胰 ...
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#44Rotavirus concentration from cell culture harvests: trypsin ...
An 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of MA-104 cells were treated with 50 microgram of trypsin per ml and ...
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#45Cell Culture Reagents » Trypsin
Trypsin is a porcine pancreas-derived enzyme this is typically used for the dissociation and disaggregation of "anchorage-dependent" mammalian cells…
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#46傳代培養 - 百科知識中文網
傳代培養,當原代培養成功以後,隨著培養時間的延長和細胞不斷分裂,一則細胞之間相互 ... 作用後,加入適量含血清之新鮮培養基終止trypsin作用,離心後再吸掉上清液。
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#47Trypsin-EDTA (0.05%) in HBSS (1x), with Phenol Red
The addition of phenol red serves as a pH indicator to adjust to the perfect cell culture conditions. The concentration of trypsin necessary to dislodge cells ...
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#48發展簡史,套用前景,分類,步驟,培養基配製,滅菌- 細胞培養步驟
步驟: 附著型細胞adherent cell 吸掉舊培養液。 用Dulbecco' 洗滌細胞至二次。 加入trypsin EDTA溶液: 方法:37℃或室溫作用數分鐘,於倒立顯微鏡下觀察,當細胞將要 ...
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#49Trypsin / EDTA
Trypsin / EDTA Solution with a having of 0.04% / 0.03%. Available in different sizes. - Human Primary Cell Culture, Supplements and Reagents.
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#50Cell culture protocol: Subculture of adherent cell lines
The degree of adhesion varies from cell line to cell line but in the majority of cases proteases e.g. trypsin, are used to release the cells from the flask.
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#51TRYPSIN-EDTA SOLUTION 10X CELL CULTURE
TRYPSIN -EDTA SOLUTION 10X CELL CULTURE & - Find MSDS or SDS, a COA, data sheets and more information.
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#52人類細胞治療產品查驗登記審查基準
由於新興生物技術之快速發展,細胞培養、保存等技術逐漸成 ... 族群倍增時間、細胞形態、繼代培養細胞滿度或細胞 ... on the use of porcine trypsin used in the.
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#53Trypsin, 1-250, >250 USP U/mg
Trypsin, 1-250. ... Trypsin is used to re-suspend cells adherent to the cell culture dish wall ... Trypsin can be used to break down casein in breast milk.
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#54Trypsin-EDTA (0.25%) Solution
Trypsin -EDTA (0.25%) is recommended for the dissociation of adherent cells, cell aggregates, and tissues into single-cell suspensions.
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#55Trypsinization
When added to a cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel. Trypsinization is often used to pass cells to ...
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#56Trypsin-EDTA (0.5%), no phenol red, 10X
Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin ...
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#57Trypsin 0.5% - EDTA 5.3 mM (10X) B81210
In cell culture research and cell biology studies enzymes are widely used to dissociate tissues and cell monolayers. The goal of cell isolation is to maximize ...
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#58Recombinant Trypsin Solution
SKU: N/A Categories: Human mesenchymal stem cell culture, Human pluripotent stem cell culture, Trypsin and Cell Dissociation Reagents ...
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#59微載體, 均一大孔徑, 高連通和通透性孔洞, 可溶解, 百分 ...
培養細胞 時,微載體高表面積提供細胞更多貼附面積,大孔洞結構可使細胞貼附於微載體的外部 ... 另外微載體可以用胰蛋白酶(Trypsin)溶解,可幾乎100%的回收活力細胞。
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#60Use of Roche Recombinant Trypsin for cell culture ...
In this application note, we demonstrate the use of Roche Recombinant Trypsin in cell culture applications, such as the detachment of adherent cells, in ...
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#61Trypsin/EDTA Solution, 0.05% (T/E) Catalog #0183
0.05% Trypsin/EDTA is used to detach adherent cells from a culture surface. T/E is for research use only. It is not approved for human or animal use, ...
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#62細胞培養步驟- 细胞培养维基百科,自由的百科全书
細胞培養 分為兩大類:貼壁培養adherent culture又稱單層培養、懸浮 ... 傳代培養中的細胞3加入trypsin EDTA溶液,37℃作用數分鐘,於倒立顯微鏡下 ...
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#63Enzymes for cell culture
Trypsin is used in tissue dissociation, cell harvesting, subculturing and other processes. Unlike native trypsin, our recombinant, trypsin for cell culture ...
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#64Corning® Trypsin
Product Number25-052-CI, Product NameCorning® 100 mL Trypsin EDTA 1X, Qty./Pk 6 / Pk, Qty./Cs 6 / Cs, Brand Corning®, Price $66.35, Unit Size per case.
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#65r-Trypsin 1x with EDTA Solution in Phosphate Buffer for cell ...
23560. r-Trypsin 1x w/ EDTA Soln in Phosphate Buffer for cell culture (AOF),150U/ml,Endotoxin (BET) 1EU/ml. Part B1 Storage : 2 to 8°C (Refrigerate)
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#660.25% Trypsin Solution
125mL. Price: Inquire. Qty: Welcome to order from local distributors. Protocols for cell culture.
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#67胰酶(Trypsin-EDTA 0.25%)(含酚红)
概述胰酶(Trypsin-EDTA)是最常用的细胞分离试剂,为223个氨基酸组成的单链多肽,是胰 ... 移除细胞培养瓶/皿中的培养液,用适量不含钙和镁离子的PBS或D-PBS快速冲洗 ...
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#68细胞传代培养常见问题合集
去掉原T25培养瓶里面的培养基,T25瓶加3-4 ml PBS洗1-2次;弃PBS,再加1.5 ml的Trypsin-EDTA (1X)消化液消化细胞,显微镜下观察,待细胞变圆,细胞间隙明显,部分细胞刚 ...
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#69Trypsin-EDTA Solution, 1X - 30-2101
Trypsin -EDTA solution is suitable for most but not for all adherent cell lines. This product has applications for cell culture, cell growth, and viability. For ...
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#70guideline-use-porcine-trypsin-used-manufacture-human ...
This includes e.g. (1) trypsin used as a reagent for cell culture during manufacture of vaccines, advanced therapy medicinal products or other.
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#71Trypsin EDTA (0.5%), EDTA 0.2% , 10X Conc. 胰酶,不含酚红
作为国内专业化的细胞培养平台,我们提供各类干细胞干细胞,免疫细胞和原代细胞无血清培养基;ALDEFLOUE试剂盒和CFU甲基纤维素半固体培养基以及组织透明剂Rapiclear.
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#72生物細胞培養標準作業程序
Medium - 培養基; Fetal Bovine Serum (FBS) - 胎牛血清; Phosphate buffered Saline (PBS ) -磷酸鹽緩衝溶液; Flask - 細胞培養盤 ...
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#73005wv Trypsin Solution inc Phenol Red ...
0.25w/v% Trypsin Solution with Phenol Red 100ml for cell culture. Product code: 201-18841. Pack Size: 100ml. CE Verification: Research Use Only.
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#74Subculture Reagents
Trypsin Xeno-Free, Trypsin/EDTA and PBS, Trypsin/EDTA Xeno-Free, Phosphate Buffered Saline, Trypsin 0.05% EDTA 0.02%.
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#75胰蛋白酶,组织细胞消化的理想之选知乎簡- trypsin 作用 - F5V8G
Trypsin 还促进细胞增殖和分化。Trypsin 可用于伤口愈合和神经源性炎症的胰蛋白酶EDTA溶液:又称培养细胞消化液,用于培养细胞的消化,或者些组织的消化。
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#76FG301-01
Trypsin contains porcine trypsin (0.25%) , EDTA ( +/-), and phenol red. It does not contain calcium and magnesium ion. Trypsin solution has been filter- ...
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#77Trypsin/EDTA 0.25% Solution
Trypsin solutions are used widely for dissociation of tissues and cell monolayers and we can deliver solutions that will perform consistently, batch after batch ...
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#78I X Trypsin EDTA
I X Trypsin EDTA Solution ( 0.05% Trypsin / 0.53 mM EDTA in HBSS), sterile filtered & tissue culture tested including stem cells.
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#79Recombinant human Trypsin-1, transfected HEK293 cell ...
Recombinant human Trypsin-1 protein overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells (200 µl).
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#80Trypsin/EDTA Solution, 100 mL
Trypsin /EDTA. ... Trypsin/EDTA 0.025% solution for use in cell culture. Scale Prices. ##PRICE## per item when you buy more than ##QTY##. 72.00 CHF.
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#81Trypsin-EDTA Solution 0.5% (10X)
Trypsin -EDTA Solution 0.5% (10X). 0.5% trypsin, 5.3mM EDTA, 8.5g/L NaCl. Without phenol red. Contact for Details. TRL02 Categories: Animal Cell Culture, ...
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#82Sustained exposure to trypsin causes cells to transition into ...
During cell culture, trypsin, a serine protease, is applied to cells for 5-10 minutes to separate them from each other and from the ...
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#83胰酶0.5% Trypsin-EDTA,无酚红,10倍浓缩工作液
胰酶0.5% Trypsin-EDTA,无酚红,10倍浓缩工作液. 产品编号:15400054 品牌:invitrogen 原厂货号:15400054; 产品分类:细胞,细胞培养与转染试剂 > 细胞解离试剂 > 胰 ...
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#84Culture Guidelines of Adherent Cells using Trypsin
Consumables and disposables used in cell culture operations including but not limited to; liquid media and additives, serum, sterile disposable culture vessels ...
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#85Trypsin/Detach kit
C-41310. PCI. PromoCell社. Accutase-Solution, primary human cell culture tested. 100 ml, ¥12,000
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#86Trypsin-induced proteome alteration during cell subculture in ...
Trypsin -digested MCF-7 cells were then either directly neutralized with 10% FCS followed by performed cell lysis or reseeded onto cell culture ...
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#870.25%胰蛋白酶-EDTA消化液(含酚红)Trypsin-EDTA ...
a. 吸去培养液,用无菌的PBS、Hanks 液或无血清培养液洗涤细胞一次,以去除残余的血清。 b. 加入少量胰酶消化液,略盖过细胞即可,室温放置30 秒至2 分钟。不同的细胞消化 ...
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#882.5% Trypsin-EDTA Solution 10X | CAS 9002-07-7 (solid)
Due to its digestive strength, 2.5% Trypsin-EDTA Solution 10X is also widely used for cell dissociation, routine cell culture passaging, and primary tissue ...
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#89Cell culture guidelines
Change media to get rid of any residual trypsin. 9. Sub-culturing of suspension cell lines. 1. Check guidelines for the cell line for recommended split ratio or ...
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#90Trypsin 1:250 (powder) 胰蛋白酶1:250(粉末)
本品是来源于猪胰腺的胰蛋白酶冻干粉,经γ射线处理灭活病毒,酶活≥ 250 units/mg。广泛用于细胞传代,将贴壁细胞从培养板上消化水解下来制备单细胞悬液,常用的工作 ...
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#91Introduction to cell culture, splitting cells using ... - YouTube
Introduction to cell culture, splitting cells using trypsin and counting them using a hemocytometer. 69K views · 2 years ago ...more ...
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#92Trypsin-EDTA
Trypsin -EDTA solution is a mixture commonly used for cell and tissue dissociation. Trypsin is a digestive protease, usually of porcine origin, ...
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#93细胞培养常用的溶液之消化液
一、胰蛋白酶(trypsin)溶液胰蛋白酶是白色或淡黄色粉末,低温干燥保存。主要作用是使细胞间的蛋白质水解,使细胞离散。其活性以其消化酪蛋白的能力 ...
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#94Trypsin EDTA Solutions (Porcine-Derived) | [Cell Culture] ...
接着細胞の剥離や各種組織の細胞分散などにご利用ください。 ... 0.05w/v% Trypsin-0.53mmol/L EDTA・4Na Solution with Phenol Red. for Cell Culture.
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#95Trypsin and anaerobic bacteria boost SARS-CoV-2 Delta ...
The team used reverse transcriptase-polymerase chain reaction (RT-qPCR) to quantify virus titer in the cell culture supernatants of VeroE6 cells ...
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#96貼附在培養基底部的細胞能分離
細胞 小劇場第三話Trypsin https://www.instagram.com/rjlab0128/ 有些貼附細胞可以用培養基或是緩衝液體沖洗下來,但大部分需要用胰蛋白(...
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#97Laurus Bio | An integrated research-driven biomanufacturing ...
Animal-Origin-Free Solutions · Cultured Meat · Cell Culture Media · Biologics and Insulin Manufacturing · Vaccine · Stem Cell Culture and Regenerative Medicine.
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#98Efficient Generation of Chondrocytes From Bone Marrow– ...
Therefore, it is essential to consider the optimal environment for these sophisticated cellular processes. Although cell culture plates have ...
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