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[爆卦]spei-hf是什麼？優點缺點精華區懶人包
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#1SpeI-HF® | NEB
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample ...
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#2SpeI-HF ® #R3133 - NEB UK
SpeI -HF #R3133 · Unit Definition. One unit is defined as the amount of enzyme required to digest 1 μg of pXba-XbaI DNA in 1 hour at 37°C in a total reaction ...
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#3SpeI-HF® - BIOKÉ
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample ...
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#4SpeI-HF - LabMal
SpeI -HF. RM420.00 – RM1,535.00. Brand: New England Biolabs. High-Fidelity (HF) restriction enzymes have the same specificity as native enzymes, ...
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#5New England Biolabs, Inc. SpeI-HF – 2500 units
Shop New England Biolabs, Inc. SpeI-HF – 2500 units at Fishersci.com. ... High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; ...
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#6SpeI-HF®, Restriction Enzymes, New England Biolabs - VWR
An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% ...
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#7NEB R3133 SpeI-HF - 科邦邦商城
NEB R3133 SpeI-HF. ... 大肠杆菌菌株，携带有克隆自球衣菌属（Sphaerotilus）（ATCC 13923）并经修饰的SpeI 基因. 单位定义. 一个单位是指在50 μl 的总反应体系 ...
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#9SpeI-HF - 佑研匠簇网上商城
SpeI -HF. 产品编号：R3133S 品牌：New England Biolabs 原厂货号：R3133S; 产品分类：; 应用分类：. 包装：. 运保温度: 到货周期：, 登录后查看. 标准价：, ￥客户可见.
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#10SpeI-HF限制性内切酶 - 丁香通
上海康朗生物科技有限公司发布在丁香通的SpeI-HF限制性内切酶SpeI-HF Restriction Endonuclease报价、型号、品牌等供应信息介绍，丁香通致力为您提供最优质的SpeI-HF ...
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#11Restriction Cloning Using SpeI-HF - Biocompare
I have used SpeI-HF restriction enzyme from NEB to digest the plasmid called pSSPgl of a size 24 Kb. Digestion resulted in 23 and 1 Kb DNA ...
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#12SpeI-HF® (Time-Saver™)
SpeI -HF® (Time-Saver™). Производитель: New England Biolabs Inc. Артикул, Название товара ...
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#13SpeI-HF - Restriction enzymes - BioNordika Denmark A/S
SpeI -HF. You must be logged in to be able to place orders. New England Biolabs. SpeI-HF | 500 units. Model/Product no.: NEB-R3133S. Add to wish list ...
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#14Anza Restriction Enzymes | Thermo Fisher Scientific - TW
Incubation was done at 37°C for 5 minutes for Competitor N SpeI-HF, ... C – undigested plasmid DNA; 1 – Anza 3 BcuI; 2 – SpeI-HF (Competitor N) ...
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#15SpeI-HF - New England Biolabs - iBioTech.cz
SpeI -HF - New England Biolabs ; R3133M, 100 000 U/ml, 2 500 U ; R3133S, 20 000 U/ml, 500 U ...
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#16SpeI (R0133) - New England Biolabs Japan
性能を向上しつつも、同価格で提供しているSpeI-HFのご使用をお勧めします！ ... (ATCC 13923)からクローニングされたSpeI遺伝子を有するE. coli株。 付属試薬：.
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#17SpeI-HF – Lab-JOT® Ltd. Sp.z o.o. Sp.k.
SpeI -HF. ilość SpeI-HF. Dodaj do koszyka. Numer katalogowy: R3133S Kategorie: Enzymy, Enzymy restrykcyjne, New England Biolabs Tag: New England Biolabs.
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#18SpeI-HF - New England Biolabs - SmartMart.bio
SpeI -HF ; R3133S, 20 000 U/ml, 500 U · Sleva 30 %. 2 260 Kč1 582 Kč ; R3133L, 20 000 U/ml, 2 500 U · Sleva 30 %. 9 090 Kč6 363 Kč.
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#19Restriction Enzymes SpeI / BcuI - Labettor
Find peer reviewed products to perform Restriction Enzymes SpeI / BcuI. ... and 0.5 μl of restriction endonuclease, SpeI-HF, was added to the beads, ...
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#20Golden Gate assembly of BioBrick-compliant parts using Type ...
The compatible SpeI and XbaI overhangs anneal to ... and Type II restriction endonucleases SpeI-HF, XhoI, XbaI and SalI-HF (5 units each) in ...
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#21Methylase-assisted subcloning for high throughput BioBrick ...
For similar reasons, 2RM assembly utilizes the components of two restriction modification systems: restriction endonucleases XbaI and SpeI-HF, ...
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#22Plasmid #: 75268 pAAV-CRY2-CreN - Addgene
DNA digested with SpeI-HF single digest, NotI-HF single digest, and SpeI-HF / NotI-HF double digest for 1.5 hours. Gel: 1% agarose stained with ethidium ...
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#23SpeI - New England Biolabs, Inc.: R0133S | Labscoop.com
SpeI has a High Fidelity version SpeI-HF® (NEB #R3133). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer ...
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#24的SpeI-HF®|鼻 - manbetx体育网页
的SpeI-HF® ... 高保真（HF）限制性内切酶具有与天然酶相同的特异性，但在单一缓冲液中显著降低星活性和 ... 所有HF-限制性内切酶来与凝胶样染料，紫色（6X）。
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#25RSC - eStore. (S) SpeI
PackageSize, 500 units. Remark, Disabled: Product has been discontinued. Alternative: High Fidelity version, SpeI-HF® (NEB #R3133).
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#26Team:NTHU Taiwan/Protocol - iGEM 2020
EcoRI-HF, SpeI-HF, PstI and XbaI-HF are often used enzyme in iGEM. Concentration of restriction enzyme should be lower than 5% to avoid star activity.
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#27Cica-Web - SpeI-HF - 製品詳細
SpeI -HF. SpeI-HF. 価格, ¥10,000. ※価格は税抜きです。 非在庫. 製品番号, 49897-52. 包装, 500units. 製品情報. 冷凍保存 －20℃以下.
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#28S1 Appendix Plasmid construction - DOI
SpeI -HF. IHDypi2. Template. Oligo/Enzyme 1. Oligo/Enzyme 2. PCR Product. IHDypg1. YellowU6_3_gRNA_F YellowPU6_3_gRNA_R. PCR Product. Genomic DNA.
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#29SpeI-HF-北京百灵克生物科技有限责任公司世界杯买球胜负平
SpeI -HF. 2020/2/4 14:44:35 浏览：. 【品牌】: NEB; 【产品货号】: R3133V; 【价格】: 359 元; 【规格】: 250 units; 【货期】: 请咨询; 在线订购. 产品介绍 ...
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#30M.XbaI and M.Ocy1ORF8430P protect plasmids from XbaI and ...
Each purified enzyme was reacted in vitro with XbaI or SpeI-HF, and the extent to which each was cut was assessed by agarose gel electrophoresis.
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#31Generation of transgenic lines - Bio-protocol
... by restriction enzymes NotI-HF and SpeI-HF (New England Biolabs). Backbone vector pTol2 were digested by the same enzyme to create the same sticky ends.
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#32大阪 店舗 アズワン(AS ONE) SpeI-HF R3133L 1セット ...
ファーストPayPayモール店 | アズワン(AS ONE) SpeI-HF R3133L 1セット.
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#33Glutathione 調控單胞藻(Chlamydomonas reinhardtii) 強光馴化
使用SpeI-HF 限制酶切割vector (pChlamyiRNA3) (Chlamydomonas. Resource Center (http://www.chlamycollection.org/)，以利後續vector 與ds. DNA oligo 黏合作用。
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#34Supplementary Information iFLinkC - Semantic Scholar
BtsI, BsrDI and either SpeI or EcoRI: The N-terminal DNA fragment is ... Depending on assembly strategy, add 1 µL SpeI-HF (20 U/µL) or EcoRI-HF (20 U/µL).
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#35FastDigest Restriction Enzymes
1: undigested plasmid DNA. 2: SpeI-HF. 3: XbaI. 4: NdeI. 5: SalI-HF. 6: AvrII. 7: BsaI. 8: EaqI-HF. 9: BglI. Plasmid DNA digested with NEB enzymes.
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#36Anza Restriction Enzyme Cloning System - Fisher Scientific
Anza restriction enzymes provide superior performance. Plasmid DNA (6,215 bp) was digested using Anza 3 BcuI and Anza 47 Eco521, as well as Competitor N SpeI-HF ...
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#37R3133L- Item Detail - MedStore
Description, SpeI-HF® - 2500 units. Detail. Additional Information on R3133L (Supplied by NEB)Close · Show Additional Supplier Information. Sku, R3133L.
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#38SpeI-HF, ref: R3133S, 1 unidad, New England Biolabs - CNIC
SpeI -HF, ref: R3133S, 1 unidad, New England Biolabs. Fecha de publicación: Miércoles, Abril 24, 2019 - 8:00am. Fecha fin de vigencia:.
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#39商品も通販 アズワン(AS ONE) SpeI-HF R3133L 1セット ...
ファーストPayPayモール店 | アズワン(AS ONE) SpeI-HF R3133L 1セット. ... アズワン(AS ONE) HFカプセル 5号 1箱(100個入り) | セミプロDIY店ファースト.
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#40BioBrick-based 'Quick Gene Assembly' in vitro
In lane 2, the high-fidelity restriction enzyme SpeI (SpeI-HF) in digestion premix was replaced by a standard SpeI enzyme. The yield of the ...
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#41SpeI-HF (100.000 units/ml) BioLabs 型番 R3133M aso 2-4303 ...
製品名：SpeI-HF (高濃度品)○容量：2.500 units○BioLabs○型番：R3133M○製品英語ページはこちら。[製品情報]○○※国内外の在庫状況により納期が表記と異なる事が ...
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#42BglII - an overview | ScienceDirect Topics
Digest the PCA product with restriction enzymes BglII and SpeI-HF and digest pLMB51 with BamHI-HF and XbaI. For New England Biolab-purchased enzymes, ...
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#43NEB Freezer - DESY Confluence
PCR ; PacI, R0547S ; SacI-HF, R3156S, 20000 ; SaII-HF, R3138S, 20000 ; SpeI-HF, R3133S, 20000 ...
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#44Day 1: DNA manipulation - Rice University
1 µl SpeI-HF® (Catalog # R3133S) and 1 µl PstI-HF® (Catalog # R3140S) to R0040 ... the vector can recircularize--digestion with SpeI and PstI only removes a ...
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#45最大50%OFF アズワン(AS ONE) SpeI-HF R3133S 1セット ...
ファーストPayPayモール店 | アズワン(AS ONE) SpeI-HF R3133S 1セット.
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#46公式販売品 SpeI-HF (100.000 units/ml) BioLabs aso 2-4303 ...
9/19-24 P最大24倍☆【全国配送可】-SpeI-HF (100.000 units/ml) BioLabs 型番 R3133M aso 2-4303-70 ○在庫品 納期約 3営業日-【医療・研究機器】 ...
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#47bglii restriction enzyme - Durbuy tourisme
... 20mM acetic acid, 1mM EDTA, pH 8.0), Restriction enzymes BamHI-HF, XbaI, BglII, SpeI-HF, and associated buffers, Lysogeny broth (LB) medium and LB-agar ...
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#48Spe I from Sphaerotilus species - Sigma-Aldrich
SPEI -RO. Roche. Spe I. from Sphaerotilus species. Spe I from Sphaerotilus species. All Photos(1). Synonym(s): SPE I. Pricing and availability is not ...
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#49HF-60SS 面背單木吉他
HF -60SS 面背單木吉他. NT$16,600. Category: 日本Headway Tags: headway, hf-60ss, solid, universe. Description. Description. Specification.
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#50Linkers in Biomacromolecules - 第 238 頁 - Google 圖書結果
Restriction Enzymes BtsI (R0667S), BsrDI (R0574S), EcoRI-HF (R3101S), SpeI-HF (R3133S), NheI-HF (R3131S) and DpnI (R0176S) all from New England Biolabs ...
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#51Marine Enzymes and Specialized Metabolism - Part A
... TAE buffer (40mM Tris, 20mM acetic acid, 1mM EDTA, pH 8.0) • DNA gel extraction kit • Restriction enzymes BamHI-HF, XbaI, BglII, SpeI-HF, and associated ...
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#52Synthetic Biology: A Lab Manual - 第 172 頁 - Google 圖書結果
DreamTaqTM DNA polymerase, 2 ThermoScientificTM 200 U EcoRI-HF, ... PstI-HF, heat-inactivatable, 10000 U 1 N.E. BiolabsTM SpeI-HF, heat-inactivatable, ...
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#53SPEI-HF®|鼻 - 必威安卓APP
SPEI -HF. ®. 克隆在Neb. 重组 · 工程 · 节省时间 · neb31 · dil_C · 37 · 80热量. 我们很高兴地宣布，我们正在切换所有反应缓冲液的过程中以BSA。从4月2021年开始，我们 ...
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#54Bibliographie der deutschen Zeitschriftenliteratur: mit ...
... H : Rachen Spei- HF : Strophanti R : Mumien Haussler , JW : Entropie chel Hancken : Syphilis Hadbank - Dunikowski , E.v : Luft - Temperatur - Tur .
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#55Bibliographie der deutschen Zeitschriftenliteratur, mit ...
... H : Rachen - Spei- HF : Strophanti R : Mumien Hæussler , JW : Entropie chel Hancken : Syphilis Hadbank - Dunikowski , E.v : Luft - Temperatur - Tur .
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#56Bibliographie der deutschen Zeitschriften-literatur
... L : Mädchen Häusgen , W : Tarifverträge Halasz , H : Rachen Spei- - HF : Strophanti R : Mumien Hæussler , JW : Entropie chel Hancken : Syphilis Hadbank ...
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