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[爆卦]NotI-HF是什麼？優點缺點精華區懶人包
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#1NotI-HF® | NEB
Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering ...
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#2NotI-HF ® #R3189 - New England Biolabs
NotI -HF #R3189 ; High-Fidelity (HF) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star ...
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#3NotI-HF® - BIOKÉ
Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering ...
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#4Datasheet for NotI-HF™ (R3189; Lot 0031208)
Quality Controls. Ligation: After 10-fold overdigestion with NotI-HF,. > 95% of the DNA fragments can be ligated with T4.
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#5New England Biolabs, Inc. NotI-HF – 500 units | Fisher Scientific
Shop New England Biolabs, Inc. NotI-HF – 500 units at Fishersci.com. ... High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; ...
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#6NotI-HF®, New England Biolabs | VWR
An E.coli strain that carries the cloned and modified NotI gene from ... NotI HF® is engineered with performance in mind, HF restriction enzymes are fully ...
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#7NotI-HF® - New England Biolabs, Inc.: R3189S | Labscoop.com
NotI -HF®: R3189S by New England Biolabs, Inc. at Labscoop.com - Read reviews, citations, datasheets, protocols & more.
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#8NotI-HF, NEB - LabMal
NotI -HF, NEB has the same specificity as NotI, but has been engineered for significantly reduced star activity and performance in a single buffer (CutSmart ...
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#9NotI-HF | New England Biolabs
NotI -HF, Article Number: R3189. ... NotI-HF. Packaging Unit: 2.500 units. € 292,00. -. +. Add to Cart. R3189M. NotI-HF, conc. Packaging Unit: 2500 units.
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#10NotI-HF（星选酶） - 喀斯玛商城
NotI -HF（星选酶） ... High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and ...
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#11NotI-HF® - Restriction Enzymes NotI | Labettor
Protocol tips. The extracted plasmid and M13-NotI-Kan vector were digested by KpnI-HF and NotI-HF. Downstream tips. The desired products were isolated by ...
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#12FastDigest NotI - Thermo Fisher
Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37C in 515 minutes using universal FastDigest Buffer.
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#13Noti Hf Restriction Enzymes (New England Biolabs) | Bioz
Noti Hf Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations.
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#14NotI-HF | 500 units - Restriction enzymes - BioNordika
Home; /; Shop; /; Products; /; Molecular biology; /; Restriction enzymes; /; NotI-HF | 500 units. NotI-HF | 500 units. You must be logged in to be able to ...
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#15ENZIMA NOTI-HF - 500 U - uniscience
Enzima de restrição recombinante de alta fidelidade NotI-HF proveniente de Nocardia otitidis-caviarum (ATCC 14630). Sítio de Clivagem: 5 ...CCC_GGG...
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#16NotI-HF RE-Mix (R5189) - New England Biolabs Japan
NotI -HF RE-Mixは直ぐに使用可能な10Xマスターミックスであり、NotI-HF (NEB #R3189)、NEBuffer、BSA、及びローディングダイを含む。H2OとDNAと混ぜ、15分間反応する ...
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#17NotI-HF (High Fidelity) - New England Biolabs | iBioTech.cz
Úvod » Reagencie » Molekulární biologie » Restrikční endonukleázy » Produkt NotI-HF (High Fidelity). SmartMart.bio. Reagencie · Molekulární biologie.
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#18NotI-HF® (Time-Saver™)
NotI -HF® (Time-Saver™). Производитель: New England Biolabs Inc. Артикул, Название товара ...
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#19NotI Enzyme | Abbexa Ltd
NotI is expressed and purified from E. coli that carries the recombinant NotI gene. The reaction is conducted for 5-10 minutes at 37 °C, ...
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#20NotI-HF (High Fidelity) - New England Biolabs | Smartmart.bio
NotI -HF (High Fidelity). Výrobce: New England Biolabs. Do oblíbených. Položit dotaz Odeslat emailem. Produkt na stránkách výrobce · Zobrazit na iBioTech.cz ...
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#21abx071039 NotI-HF Enzyme - 瑞柏生物科技股份有限公司
型號：abx071039. 中文名稱：NotI-HF Enzyme. 英文名稱：NotI-HF Enzyme. 包裝單位：250 / 500 units. 廠牌：abbexa. 售價：與我們聯絡. 產品說明：相關網頁 ...
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#23GEO Accession viewer - NCBI
Reactions consists of 5 U NotI-HF (NEB catalog no. R3189L), 5 µg library plasmid DNA, 1 X NEBuffer 4 (supplied with enzyme) and 90 µl of ...
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#24noti-hf enzima 20000u/ml 2500u bugiardino cod: 963397981
bugiardino di noti-hf enzima 20000u/ml 2500u new england biolabs parafarmaco sanitario altri prodotti a valenza sanitaria previsti dalla tabella ...
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#25NEB - Freezer Program - University of Maine
Restriction Enzymes ; EcoRI – HF, R3101S, 10,000 U ; HindIII – HF, R3104S, 10,000 U ; MseI, R0525S, 500 U ; NotI – HF, R3189S, 500 U ...
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#26Not I from Nocardia otitidis-caviarum | Sigma-Aldrich
NOTI -RO. Roche. Not I. from Nocardia otitidis-caviarum. Not I from Nocardia otitidis-caviarum. All Photos(1). Pricing and availability is not currently ...
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#27Vendor Stock Listings - DNA Core
Enzyme Catalog Number Units Price Stock Qty 1 kb DNA Ladder N3232L 1,000 gel lanes $209.00 1 1 kb DNA Ladder N3232S 200 gel lanes $53.20 1 1 kb Plus DNA Ladder N3200S 100 gel lanes $57.00 1
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#28NotI-HF-北京百灵克生物科技有限责任公司
NotI -HF. ... NotI-HF. 2020/2/4 15:31:35 浏览：6. 【品牌】: NEB; 【产品货号】: R3189V; 【价格】: 389 元; 【规格】: 250 units; 【货期】: 请咨询; 在线订购.
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#29Escherichia coli Swimming back Toward Stiffer Polyetheylene ...
cleanup column (Zymo Research), the NotI-HF digested PCR product and pflhDC plasmid were ligated. together with T4 DNA ligase in ligase ...
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#30R3189S, NotI-HF (High Fidelity) - 500 units - BioZyme
R3189S, NotI-HF (High Fidelity) - 500 units. http://international.neb.com/products/r3189-noti-hf. TVA inclus. 514,08 RON. Cod SKU (T). NEB_R3189S.
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#31S1 Appendix Plasmid construction - PLOS
NruI-HF. IHDypi1. Template. Oligo/Enzyme 1. Oligo/Enzyme 2. PCR Product. Genomic DNA ... NosCas9_2_F. NosCas9_2_R. Plasmid Digest IHDyi2. NotI-HF. SphI-HF.
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#32NotI-HF限制性内切酶 - 北京唐川科技有限公司
产品名称, NotI-HF限制性内切酶. 英文名称. 英文别名. CAS No. 分子式. 分子量. 精确质量. PSA. LogP. ×. 大包装询价. CAS NO. 包装规格. 纯度. 数量. 单位名称.
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#33Sheet1 - bioRxiv
Restriction enzymes NotI-HF and BamHI-HF were used to digest pks mNeonGreen N11 NEO vector; Gibson reaction was used for assembly.
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#34Restriction Digest - an overview | ScienceDirect Topics
NotI -HF (and most other enzymes) is also active in 1× CutSmart Buffer at 37°C. Once this is confirmed, you can set up your digest accordingly.
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#35I would like to digest 35 μg plasmid DNA with NotI-HF. How ...
Theoretically, it can be done in two reactions if I use 1 μL NotI-HF 20 u/μL, since 1 unit is enough for 1 μg DNA. But what are the optimum conditions?
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#36その他 NotI-HF R3189L 2-4303-45 納期目安 ウイーク
46552円 ウイーク 薬・連繋・介護 NotI-HF 2-4303-45 その他 納期目安 その他種々の物 R3189L.
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#37Design of a functional cyclic HSV1-TK reporter and its ... - Nature
R3140); NotI-HF (cat. no. ... Digest the eluted DNA using Hind III-HF and BamH I-HF enzymes with buffer 4 and BSA (NEB products).
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#38Restriction analysis of the three plasmids, integration of ...
EagI HF. NotI HF buffer (5 uL) no cutsmart cutsmart plasmid. pTPGI_dCas9. _VP64. 4 ug. 4 ug. 4 ug water up to 50 uL. Step 4: transformation protocol.
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#39New England Biolabs Inventory at the KUMC - July 2014
1,000 R3131S. $57.30. NotI HF. R. 500 R3189S. $61.85. PflFI. 2000 R0595S. $54.57. PstI-HF®. R. 10,000 R3140S. $54.57. SalI-HF®. R. 2,000 R3138S.
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#40NotI restriction enzyme - Takara Bio
NotI restriction site: GC | GGCC GC CG CCGG | CG. Supplied buffer: H+BSA+Triton X-100. Effect of DNA methylation: enzyme activity is affected by CG ...
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#41Restriction Enzymes - Catalog
R0176S. R0176S. DpnI - 1000 units (NEB). View Detail Add to Order. 14, $87.69. R3189S. R3189S. NotI-HF® - 500 units (NEB). View Detail Add to Order.
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#42Molecular Biology Techniques: A Classroom Laboratory Manual
This buffer is recommended for both NcoIHF and NotI-HF and is supplied as a 103 stock solution. The final concentration needs to be 13.
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#43NEB Freezer - DESY Confluence
NcoI-HF, R3193S, 20000, 1000, 53.60. NdeI, R0111S, 20000, 4000, 55.20. NheI-HF, R3131S, 20000, 1000, 56.80. NotI-HF, R3189S, 20000 ...
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#44EP2390316A1 - High fidelity Not1 restriction endonucleases
Figures 17A-B show cleavage of 1.2 µl pXba DNA substrate using 2-fold serial dilutions of NotI-HF (287,200 U and 19,200 U) and 2-fold serial dilutions of WT ...
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#45Fillable Online NotI-HF Fax Email Print - pdfFiller
Do whatever you want with a NotI-HF: fill, sign, print and send online instantly. Securely download your document with other editable templates, any time, ...
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#46UMASS Medical School TCEFS
R3131S NheI-HF - 1,000 units. $64.00. $52 ✓ ✓ ✓. N3232L 1 kb DNA Ladder - 1,000 gel lanes. $204.00. $165. ✓ ✓. R3189L NotI-HF - 2,500 units.
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#47Untitled - SID
For the PFGE analysis of bioluminescent bacteria, the NotI-HF™ is the method of choice among the restriction enzymes.
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#48آنزیم NotI-HF - فروشگاه اینترنتی کیاژن
آنزیم NotI-HF · Engineered for improved performance · 100% activity in rCutSmart Buffer · Time-Saver™ qualified for digestion in 5-15 minutes · Reduced star ...
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#49IGEM:Hong Kong HKUST/Investigations/Performance of NotI ...
Digest pSB1C3 using NotI-HF for 5, 15 and 30 minutes. 9. Run gel electrophoresis to obtain the investigation results, and to examine the ...
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#50RE-Mix™ Restriction Enzyme Master Mixes
EcoRV-HF™ RE-Mix™. R5195. 200. KpnI-HF™ RE-Mix™. R5142. 200. MfeI-HF™ RE-Mix™. R5589. 25. NcoI-HF™ RE-Mix™. R5193. 50. NheI-HF™ RE-Mix™. R5131. 50. NotI-HF™ ...
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#51High fidelity restriction endonucleases - European Patent Office
Figures 17A-B show cleavage of 1.2 Pl pXba DNA substrate using 2-fold serial dilutions of NotI-HF (287,200 U and.
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#52Noti-HF的具体活动是什么？|鼻
The specific activity of NotI-HF in the recommended NEBuffer 4 is 5.5 x 10 5 Units/mg. The specific activity of NotI wild type in the ...
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#53Index verborum quae in Senecae Fabulis necnon in Octavia ...
noti HF 1136 ; nostris ( m . ) Tr 74 , M 769 , 0e 172 ; ( f . ) HF notae ( 8. ) Pha 1056 ; notum Pho 106 , Th 998 , HO 1462 ; ( neu . ) ...
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#54Chemokines - 第 199 頁 - Google 圖書結果
... vector pMS101C with the BssHII and SalI-HF restriction enzymes. ... 200 U) 20 μL SalI-HF (final amount: 400 U) 10 μL NotI-HF (final amount: 200 U) 4.
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#55The Neuronal Cytoskeleton, Motor Proteins, and Organelle ...
mGFP (or the fluorophore of choice) with 1 U NheI-HF (NEB R3131S) and 1 U NotI-HF (NEB R3189S) in the supplied CutSmart buffer for 1 h at 37 C (Figure 2(A)) ...
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#56Impact of Survivin Acetylation on its Biological Function
Polymerase BamHI-HF® - Kit TaKaRa DNA Ligation Kit Ver. ... New England BioLabs® Restriction endonu- cleases EcoRI-HF - New England BioLabs® NotI-HF -.
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#57Membrane Proteins – Production and Functional Characterization
... (SOC) medium • Autoclaved LB Miller medium • LB agar plates • Enzymes • NheI-HF (New England Biolabs) • NotI-HF (New England Biolabs) • T4 DNA ligase, ...
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#58B. FOGLIO ILLUSTRATIVO - Banca Dati Farmaci
Non noti (non possono essere definiti sulla base dei dati disponibili) ... Vistor hf. Sími: +354 519 3643. Slovenská republika.
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#59S1 Appendix Plasmid construction - DOI
NruI-HF. IHDypi1. Template. Oligo/Enzyme 1. Oligo/Enzyme 2. PCR Product. Genomic DNA ... NosCas9_2_F. NosCas9_2_R. Plasmid Digest IHDyi2. NotI-HF. SphI-HF.
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#60Flókið að bæta hratt við virkjunum - MBL
... bara allir sem koma að þessum orkuskiptum, noti þessa skýrslu, því hún er sneisafull af stöðuupplýsingum,“ segir Ari Trausti og ítrekar ...
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#61XVIII Rally Clásico de Mallorca: Más de 100 equipos ...
noti mérica ... el Bentley Old Number One de 1948, el Jaguar E-Type de 1971, el Subaru WRC de 1995 y el Lancia Delta HF Integrale de 1991.
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#62Padova: Francesca Gislon sarà la candidata sindaca di Orizzonti
Alla presentazione della candidata non solo cittadini e sostenitori di Orizzonti, ma anche alcuni volti noti e vecchie frequentazioni.
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#63Mercato Apparecchiature di processo Driver di crescita del ...
Il rapporto si concentra su provider noti nei segmenti di mercato globale Apparecchiature di processo per applicazioni, entrate e quota di ...
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