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#1外切酶- 維基百科，自由的百科全書
外切酶，或核酸外切酶（英語：exonucleases）可以是單獨存在的酶，或是大型酶複合物的一部分。此類酵素能將聚核苷酸鏈分解成核苷酸。作用方式是將3'或5'端上的磷酸酯鍵 ...
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#2Exonuclease - an overview | ScienceDirect Topics
Exonucleases comprise a large set of structurally and biochemically characterized enzymes with diverse properties. Systematic characterization of exonucleases ...
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#3Exonuclease I (E. coli) | NEB
DNA specific exonuclease; Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Exonuclease I is ideal for:.
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#4Exonuclease I (20 U/µL) - Thermo Fisher Scientific
Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise ...
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#5Exonuclease Definition & Meaning - Merriam-Webster
The meaning of exonuclease is an enzyme that breaks down a nucleic acid by removing nucleotides one by one from the end of a chain.
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#6Characterization of the human and mouse WRN 3′→5 - NCBI
Here, we further characterize the hWRN exonuclease. hWRN efficiently degraded the 3′ recessed strands of double-stranded DNA or a DNA–RNA heteroduplex. It had ...
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#7Exonuclease I - Takara Bio
coli Exonuclease I is a 3' to 5' exonuclease for single-stranded DNA degradation, resulting in the production of 5'-phosphate mononucleotides from the 3'- ...
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#8Exonuclease I, Nucleases - Jena Bioscience
Exonuclease I · removal of residual ssDNA, including oligonucleotides, from reaction mictures · does not degrade double-stranded DNA or RNA · pequires magnesium ...
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#9The 3′-to-5′ Exonuclease Activity of Vaccinia Virus DNA ...
The 3′-to-5′ Exonuclease Activity of Vaccinia Virus DNA Polymerase Is Essential and Plays a Role in Promoting Virus Genetic Recombination.
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#105'-3' exonuclease (IPR002421) - InterPro entry - EMBL-EBI
Description · In prokaryotes, the essential FEN reaction can be performed by the N-terminal 5'-3' exonuclease domain present on DNA polymerase I. Some eubacteria ...
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#11Exonucleases | Biocompare.com
In molecular biology, commercial exonucleases are available for a variety of applications: Exonuclease I can be used for degrading excess ...
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#12single cleavage assay for T5 5′→3′ exonuclease
Abstract. Bacteriophage T5 5′→3′ exonuclease is a member of a family of sequence related 5′-nucleases which play an essential role in DNA replication.
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#13Exonuclease I, E. coli - Lucigen
We provide a wide range of DNA & RNA modifying enzymes for molecular biology and Next-Gen Sequencing applications. Click here to learn more.
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#14Insight into mechanisms of 3′-5′ exonuclease activity and ...
AP endonucleases are multifunctional DNA repair enzymes that possess AP site nicking, 3′ repair diesterase, NIR, and 3′→5′ exonuclease ...
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#15T5 Exonuclease - NZYTech
T5 Exonuclease is a typical DNA nuclease that functions in the 5´ to 3´ direction and attacks both single and double-stranded DNA. The enzyme substrate is the 5 ...
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#165′ Exonuclease Activity of Human AP-Endonuclease APE1
In addition to the AP-endonuclease activity, APE1 possesses 3′-5′ exonuclease activity, which presumably is responsible for cleaning up nonconventional 3′ ...
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#17Exonuclease 1 - Homo sapiens (Human) - EXO1 gene & protein
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair ...
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#18Exonuclease III - Promega Corporation
The enzyme is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments. Exonuclease III is provided with ...
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#19Exonuclease I Cytiva E70073Z, pack of 2500 U - Sigma-Aldrich
Exonuclease I (EXO1) maintains genomic stability by participating in DNA mismatch repair system, DNA recombination, DNA replication and DNA double-stranded ...
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#20Exonuclease of Human DNA Polymerase ε by Fludarabine ...
We conclude that the 3′→ 5′ exonuclease of human DNA polymerase ε can remove 3′-terminal F-ara-AMP from DNA with difficulty and that this excision results ...
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#21Exonuclease I (E. coli) | Canvax Biotech
Exonuclease I, the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3′→5′ ...
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#22Dynamic coordination of two-metal-ions orchestrates λ ...
λ-exonuclease translocation along DNA is driven by the chemical free energy released by the hydrolysis of phosphodiester bonds and is a highly ...
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#23Structures of Human Exonuclease 1 DNA Complexes Suggest ...
Summary. Human exonuclease 1 (hExo1) plays important roles in DNA repair and recombination processes that maintain genomic integrity. It is a ...
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#241EXN: T5 5'-EXONUCLEASE - RCSB PDB
A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease. · Entity ID: 1 · UniProt · Protein Feature View · Expand.
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#25Endonuclease vs Exonuclease- Definition, 11 Major ...
Exonuclease, like endonuclease, is a hydrolyzing enzyme that cleaves the phosphodiester bond between the nucleotides. · One of the most important ...
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#26Exonuclease I | Cytiva
Exonuclease I catalyzes sequential removal of nucleotides from the 3' end of single-stranded DNA molecules. Order Product.
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#27EXO1 - Exonuclease 1 - Function - neXtProt
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair ...
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#28Structural and Biochemical Studies of a Moderately ... - PLOS
A novel exonuclease, designated as MszExo I, was cloned from Methylocaldum szegediense, a moderately thermophilic methanotroph.
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#29Human exonuclease 1 (EXO1) activity characterization and its ...
Human exonuclease 1 (EXO1) is involved in multiple DNA metabolism processes, including DNA repair and replication.
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#30The Role of P53 Exonuclease in Accuracy of DNA Synthesis ...
Mutator phenotypes (with the potential for cancer progression) have been reported for cells that lack a proofreading 3'→5' exonuclease activity associated ...
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#31Medical Definition of Exonuclease - RxList
Exonuclease : An enzyme that cleaves nucleotide bases sequentially from the free ends of a nucleic acid (DNA or RNA).
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#32"Molecular consequences of the loss of 3' to 5' exonuclease ...
The DNA replication polymerases δ and ϵ have an inherent proofreading mechanism in the form of a 3'→5' exonuclease. Upon recognition of errant ...
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#33A novel exonuclease-assisted isothermal nucleic acid ...
Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with ...
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#34T5 Exonuclease - Intact Genomics
T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE.
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#35Directed evolution of nucleotide-based libraries using lambda
An initial PCR amplification using phosphorylated primers with overlapping regions followed by treatment with lambda exonuclease generates ssDNA ...
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#36Polymerase and Exonuclease Activities in Herpes Simplex ...
Exonuclease activity requires unwinding of two nucleotides of the duplex primer–template. Surprisingly, even though the exonuclease rate is much ...
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#37Exonuclease definition and meaning | Collins English Dictionary
Exonuclease definition: an enzyme that is capable of detaching the terminal nucleotide from a nucleic acid chain | Meaning, pronunciation, translations and ...
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#38EXO1 Gene - GeneCards | EXO1 Protein | EXO1 Antibody
Complete information for EXO1 gene (Protein Coding), Exonuclease 1, including: function, proteins, disorders, pathways, orthologs, ...
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#39Functional characterization of an alkaline exonuclease and ...
Exonucleases may be broadly divided into two functional classes: distributive enzymes that dissociate after removal of a single nucleotide; or ...
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#40Exonuclease - Proteopedia, life in 3D
Mg coordination site in E. coli exonuclease I (PDB code 1fxx). Water molecules shown as red spheres. 3D Structures of exonuclease.
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#41Exonuclease I - Enzymatics
Product Description: Exonuclease I cleaves single-stranded. DNA in the 3′→5′ direction, releasing 5′ -mono/di- nucleotides and leaving double-stranded DNA ...
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#42Combining SARS-CoV-2 Proofreading Exonuclease and RNA ...
Combining SARS-CoV-2 Proofreading Exonuclease and RNA-Dependent RNA Polymerase Inhibitors as a Strategy to Combat COVID-19: A High-Throughput in ...
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#43So Many DNA Polymerases, So Little Time | GoldBio
DNA Polymerase I · 5´ - 3´ polymerase · 3´ - 5´ exonuclease activity (proofreading) · 109 kDa · Optimal temperature is 37°C · Polymerizes 1000 nucleotides per minute.
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#44Exonuclease III (E. coli) - BIOKÉ
Exonuclease III (E. coli) M0206 New England Biolabs. ... Double-stranded DNA specific exonuclease; Initiates at the 3' termini of linear double-stranded DNA ...
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#45How is the action of exonuclease different from that of ... - Toppr
Exonuclease are enzymes that cleave the nucleotides at the end of the DNA molecule.The cleavage by exonuclease results in the formation of nucleotides or ...
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#46Endonuclease vs Exonuclease- 10 Common Differences
The main function of endonuclease is to protect a cell from foreign nucleic acid while the function of exonuclease is to help in DNA repair. The ...
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#47II. Primers & Primase. (Top of handout 11-3. Steps 5 & 6)
The enzymatic activity of DNA polymerase that removes RNA primer has a different exonuclease activity -- this enzyme removes nucleotides one at ...
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#48Exonuclease III--Novoprotein Scientific Inc. - 近岸蛋白质
例如，可将双链DNA用产生不同末端的限制性内切酶双酶切后，利用Exonuclease III的3'→5'的外切酶活性，从一端降解，得到互补的单链DNA和5'-P单核苷酸。
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#49DNA polymerase strand displacement synthesis and 3′-5
Download scientific diagram | DNA polymerase strand displacement synthesis and 3′-5′ exonuclease activity. (A) T4 DNA polymerase or Bst DNA polymerase, ...
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#50Exonuclease I (20 U/µL) - 诺为生物
Thermo Scientific Exonuclease I(ExoI），提供20 U/µL，4000 Units的包装形式，能够在3''→5''的方向上降解单链DNA。它能够逐步释放脱氧核糖核酸5'单磷酸，并留下完整 ...
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#51Difference Between Endonuclease and Exonuclease - Pediaa ...
An exonuclease is a type of hydrolases that cleave the nucleic acid chain at its end. Exonucleases remove nucleotides one by one from the ...
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#52Section II: 5'-3' Exonuclease - nostatic
The main duty of the 5'-3' exonuclease activity is to remove the RNA primers at the 5' ends of newly synthesized DNA so that the polymerase activity can fill in ...
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#53What is exonuclease activity of DNA polymerase? | Study.com
The main function of the DNA polymerase is to catalyzes the synthesis of new DNA strand identical from the parent strand. It adds the nucleotides...
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#55Class 2 Transferases XI: EC 2.7.6 - 2.7.7 - 第 122 頁 - Google 圖書結果
... [64]; <32> exonuclease activity contributes to the avoidance of alkylation mutations [71]; ~54° natural substrate is gapped DNA [82]) (Reversibility: ?
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#56Exonuclease III_百度百科
Exonuclease III即核酸外切酶III (E.coli)，該酶作用於雙鏈DNA，沿3'→5'方向逐步催化去除單核苷酸(1)。每次酶與底物結合催化，只有幾個核苷酸被除掉，從而在DNA分子羣 ...
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#57New Research Directions in DNA Repair - 第 292 頁 - Google 圖書結果
Since formation of exonuclease complexes requires “melting” of the terminal three base pairs at the primer end, the nature of mismatch at the primer end and ...
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#58Difference Between Exonuclease and Endonuclease
Explain Exonuclease. The enzymes that split a DNA sequence from either 5' or 3' end of a polynucleotide chain are called exonucleases. These nucleases are ...
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#59Exonuclease - WIKI 2
Pol I then synthesizes DNA nucleotides in place of the RNA primer it had just removed. DNA polymerase I also has 3' to 5' and 5' to 3' exonuclease activity, ...
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#60Ensemble cryo-electron microscopy uncovers conformational ...
... RNA polymerase (RdRP), helicase (nsp13), an exonuclease (nsp14), an endonuclease (nsp15), and methyl-transferases (nsp14 and nsp16).
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#61PROG NUCLEIC ACID RES&MOLECULAR BIO V2
It differs from exonuclease-II in that it carries out a limited digestion of DNA proceeding to about 40% of completion. In the case of heat-denatured DNA, ...
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#62What is exonuclease activity of DNA polymerase?
Does exonuclease need ATP? Does telomerase need a primer? Does DNA polymerase 1 or 3 come first? Is RNA transcribed 5 to 3? How is RNA ...
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#63ExS-Pure - Reliable enzymatic PCR clean-up | NimaGen
ExS-Pure is designed for fast and easy PCR cleanup. The reagent kit consists of two recombinant hydrolytic enzymes: Exonuclease-I and Shrimp Alkaline ...
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#64Solved Steps in Strand Synthesis Nick ,5' 3' exonuclease RNA
Transcribed image text: Steps in Strand Synthesis Nick ,5' 3' exonuclease RNA primer • Primer removal uw Pol sites 3' 5' exonuclease Replacing RNA with DNA ...
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#65PCR Strategies - 第 48 頁 - Google 圖書結果
A substantial difference among polymerases is the presence or absence of associated exonuclease activities. Only Taq DNA polymerase (Longley et al., 1990; ...
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#66TWiV 718: Recombining vaccine schedules - Microbe.TV
... of AZD1222 COVID-19 vaccine, safety and efficacy of Sputnik V, and control of coronavirus recombination by a viral proofreading exonuclease.
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#67Les exonucléases 3′-5 ′ - nature examine la biologie ...
De nombreuses polymérases (par exemple, les pols γ, δ et ɛ) contiennent une autre activité enzymatique dans le polypeptide de l'ADN polymérase - une exonucléase ...
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#68Advances in Cancer Research - 第 132 頁 - Google 圖書結果
The inability of N mutants to synthesize exonuclease and endolysin is not due simply to inability to produce A DNA. Mutants in cistrons, N, O, ...
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#69DNA Replication Stress - 第 206 頁 - Google 圖書結果
Notably, Mre11 contributes to DNA repair of DSB ends or protein-DNA crosslinks via its 3–5 exonuclease activity [32,47]. Accumulating evidence suggests that ...
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#70Molecular Mechanisms of Werner’s Syndrome
CHAPTER 3 Biochemical Characterization of the Werner Syndrome DNA Helicase - Exonuclease Michael Fry T Abstract he positional cloning in 1996 of WRN ...
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#71Structural basis of mismatch recognition by a SARS-CoV-2 ...
... Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3′ to 5′ exonuclease activity essential for immune suppression.
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#72Sự khác biệt giữa Endonuclease và Exonuclease - 2021
Chất nuclease là một enzyme có khả năng tách các liên kết phosphodiester giữa các nucleotide trong các axit nucleic. Endonuclease và exonuclease là hai loại ...
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#73Nucleic Acids and Molecular Biology
Divalent metal ions (Mg”, Mn”, or Zn”, but not Ca”) are essential for exonuclease activity (V. Derbyshire, unpublished 1988).
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#74Endonuclease ve Exonuclease Arasındaki Fark - strephonsays
Anahtar Farkı - Endonuclease vs Exonuclease. Endonükleaz ve eksonükleaz arasındaki farka bakmadan önce, tam olarak bir nükleazın ne olduğunu bilmek ...
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#75International Conference on Ultraviolet Carcinogenesis,
Effect of purification of calf spleen DNA polymerase a on its fidelity and capacity to copy UV - irradiated DNA , and the loss of a 3 to 5 ' exonuclease ...
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#76DNA Manipulative Enzymes
Examples - Exonuclease III. Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain that connects the RNA and DNA tracts ...
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#77Novel Genetic Causes of Gastrointestinal Polyposis Syndromes
Both proteins have proofreading exonuclease abilities, which corrects synthesized DNA for replication errors. PPAP is characterized by ...
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#78Takeda's LIVTENCITY (maribavir) Approved by U.S. FDA as ...
Accelerated Evolution of Maribavir Resistance in a Cytomegalovirus Exonuclease Domain II Mutant. J of Vir, Jan 2008, 82 (1) 246-253.
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#79Dna Polymerase - Dkfqka16.com
This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, ...
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#80A novel bayesian functional spatial partitioning method with ...
... Electrochemical microRNA Biosensor Coupling With G-Triplex/Methylene Blue Complex and λ-Exonuclease-Assisted Recycling Amplification ...
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#81人MRE11 knockout HCT116 cell line (ab286539) - Abcam中文 ...
The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A.
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#82Takeda's LIVTENCITYTM (maribavir) Approved by U.S. FDA ...
Accelerated Evolution of Maribavir Resistance in a Cytomegalovirus Exonuclease Domain II Mutant. J of Vir, Jan 2008, 82 (1) 246-253.
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#83Conduct Science
ELISA Kit for Exonuclease... $950.00. Cloud Clone... ELISA Kit for Ribosomal... $950.00. Specimen Lab... Protected: Five Fluorescence Channel... Animal Lab.
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#84MAJOR Test Neet-UG (09-09-2021) Sections Chemistry S...
The prokaryotic enzymes with 5'+3'exonuclease property. Biology. Human Physiology - General. (C) 157. The prokaryotic enzymes with ...
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#85Genome-scale transcriptional activation by an engineered ...
exonuclease degradation. To further improve the potency of Cas9-mediated gene activation, we considered how transcriptional activation is achieved in ...
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#86Glacial acetic acid for industrial use (7) - echemi topic center
3 to 5 exonuclease · 5hiaa test · abilify class. Search more information of high quality chemicals, good prices and reliable suppliers, ...
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#87Mucosal IgA Prevents Commensal Candida albicans ...
ends via exonuclease/polymerase. After 3' end adenylation a. NEBNext Adaptor with hairpin loop structure was ligated to.
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#88Takeda's LIVTENCITY TM (maribavir) Approved by US FDA as ...
Accelerated Evolution of Maribavir Resistance in a Cytomegalovirus Exonuclease Domain II Mutant. J of Vir, Jan 2008, 82 (1) 246-253.
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#89Lia Yosaatmadja (@shotliverfreak) / Twitter
The purification of active SARS-CoV-2 nsp14/nsp10 Exonuclease complex has been completed by one of our scientists, Yuilana Yosaamadja, in a collaboration ...
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#90NSP - バイオマーケットjp
S protein variation is correlated with nsp3, nsp6, and 3'-to-5' exonuclease variation. ... 1; 2 · 3.
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#91nuclease. 시장 2021 글로벌 시장 성장, 규모, 수요, 동향, 통찰력 ...
exonuclease. DNASES. rnases. 응용 프로그램 별 nuclease. 시장 세분화 : 실 혐실. 연구 기관. 기타. 지리적으로이 보고서는 2015 년 ...
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