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#1Star Activity | NEB
Under non-standard reaction conditions, some restriction enzymes are capable of cleaving sequences which are similar, but not identical, to their defined ...
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#2Differences between EcoRI Nonspecific and “Star” Sequence ...
由 NY Sidorova 著作 · 2004 · 被引用 35 次 — The binding of the restriction endonuclease EcoRI to DNA is exceptionally specific. Even a single basepair change (“star” sequence) from the recognition ...
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#3What is restriction enzyme star activity - Promega Corporation
However, under nonoptimal conditions, the differences in cleavage rates between cognate and next-best sites change dramatically for many enzymes. This loss of ...
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#4Star activity - Wikipedia
Star activity is the relaxation or alteration of the specificity of restriction enzyme mediated cleavage of DNA that can occur under reaction conditions ...
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#5EcoRI (10 U/µL) - Thermo Fisher
Thermo Scientific EcoRI restriction enzyme recognizes G^AATTC sites and cuts ... Enzymes for a table of enzyme activity, conditions for double digestion, ...
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#6A Mechanism for Star Activity of the Restriction Endonuclease ...
By cleaving DNA with EcoRI in the presence of several osmolytes, we show that the extent of this EcoRI "star activity" depends strongly upon osmotic ...
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#7Molecular recognition mediated by bound water
... water: A mechanism for star activity of the restriction endonuclease EcoRI ... Many restriction endonucleases such as EcoRI lose some specificity for ...
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#8Modulating Restriction Endonuclease Activities and ...
Assays of star cleavage of pUC18 by EcoRI,. PvuII and BamHI endonucleases in equimolar concentrations of Triton X-100 and sucrose revealed reduced star activity.
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#9Product Information: EcoRI , #ER0273 - Fisher Scientific
1X Buffer EcoRI (for 100% EcoRI digestion). 50 mM Tris-HCl (pH 7.5), ... *Star activity appears at a greater than 5-fold overdigestion (5 U × 1h).
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#10Compensating for star activity of restriction enzymes - Takara Bio
This phenomenon is called "star activity", and almost all restriction enzymes can have star activity, depending on enzymes, substrate DNA, and reaction ...
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#11LraI from Lactococcus raffinolactis BGTRK10-1, an ... - Hindawi
Modified specificity of restriction enzyme (star) activity could be ... In all endonuclease activity assays commercial EcoRI restriction ...
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#12Restriction Enzymes - Sigma-Aldrich
Star activity is defined as the alteration in the digestion specificity that occurs under sub-optimal enzyme conditions. Star activity results in cleavage of ...
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#13Osmotic and Hydrostatic Pressure Effects on BamHI, Pvu II - jstor
(protein-DNA recognition/star activity/bound water). CLIFFORD R. ROBINSONt AND STEPHEN G. ... has indicated that BamHI and EcoRI share similar structural.
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#14EcoRI, Restriction Enzymes - Jena Bioscience
Enzyme activity was determined in the recommended reaction buffer. ... glycerol concentration >5 % or pH >8.0 may result in star activity. DNA Methylation:
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#15How to reduce star activity in restriction digestion?
I am using BamH1 and EcoR1 for restriction digestion of my gene of 1.3Kb but EcoR1 is also cutting in between the gene.
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#16HF Enzymes - New England Biolabs GmbH
Reduced star activity eliminates unwanted cleavage ... EcoRI-HF® (NEB #R3101) shows no star activity in overnight digests, even when used at higher ...
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#17EP0449178A2 - Method for the reduction of star activity
To reduce non-specific star activities in the specific cleavage of ... Such star activities have been found for many enzymes such as EcoRI (EcoRI * ).
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#18BST 生化科技系生物化學實驗
EcoRI E = genus Escherichia co = species coli ... EcoRI. +. 5'--CTGCAG--3'. 3'--GACGTC--5'. 5'--CTGCA-OH. 3'--G-P. P-G--3' ... (6) 注意Star activity.
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#19COA:FastDigest EcoRI, #FD0274
FastDigest enzymes can be used to digest plasmid, genomic and viral DNA as well as PCR products and do not show star activity even in prolonged incubations.
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#20NEB Restriction Enzymes - BIOKÉ
As shown in the figure, NEB EcoRI-HF shows significantly reduced star activity in overnight digests, as compared to Thermo Scientific. Fermentas EcoRI. Figure: ...
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#21Changes in solvation during DNA binding and cleavage are ...
This change in EcoRI specificity has been termed EcoRI∗ (“star”) activity. Analogous effects have been observed in many other restriction enzyme systems and ...
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#22Molecular Biology Protocol - Restriction Digest of Plasmid DNA
Sometimes enzymes cut sequences which are similar, but not identical, to their recognition sites. This is due to "Star Activity" and can happen for a variety of ...
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#23EcoRI-HF ® #R3101 - New England Biolabs
HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the ...
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#24EcoRI - Simplebiotech Enzymes and more
EcoRI is a restriction enzyme isolated from E. coli RY 13 that cleaves the palindromic sequence ... Star activity: Conditions of low ionic strength, ...
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#25EcoRI/SpeI Double Digest - OpenWetWare
The sole reason which promted us to create the unique reaction buffer for EcoRI is avoidance of Star Activity. [Star Activity is defined as ...
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#26EP2395079B1 - Screening method - Google Patents
Star activity introduces undesirable cuts in a cloning vector or other DNA. ... Fig 7C : WT EcoRI in EcoRI buffer (see NEB catalog 2007-8).
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#27restriction enDonucleAses
Star activity is not observed with EcoRI-HF™, even at higher enzyme amounts. Reactions were set up according to recommended reaction conditions of each ...
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#28About: Star activity - DBpedia
Star activity is the relaxation or alteration of the specificity of ... activity was introduced by Mayer who characterized the modified activity in EcoRI.
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#29'Star' activity and complete loss of specificity of CeqI ...
'Star' activity and complete loss of specificity of CeqI endonuclease ... The EcoRI restriction endonuclease with bacteriophage λ DNA.
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#30Recognition and Cleavage of DNA by Typeâ•'II Restriction ...
water release reaction might be the cause of star activity (i.e. cleavage at sites that differ in ... Dependence of DNA cleavage rates by EcoRI and EcoRV on.
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#31Fidelity Index Determination of DNA Methyltransferases - PLOS
Many restriction enzymes exhibit star activity, an event in which cleavage ... Among the first reported was M.EcoRI, which was shown to only ...
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#32Ca2+-mediated Site-specific DNA Cleavage and Suppression ...
Promiscuous Activity of KpnI Restriction Endonuclease* ... first observed for EcoRI REase and termed as star activity (12).
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#33Restriction-enzymes (PD).pdf - the RLA College
For example, EcoRI* (EcoRI star activity) cleaves the sequence N/AATTN, where N is any base, whereas. EcoRI cleaves the sequence GAATTC.
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#34EcoRI ClearCut Enzyme - Genomics-online
Application: Restriction Digest (RD); Characteristics: ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity ...
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#35Fermentas Restriction Endonucleases Activity
Buffer is not recommended, since star activity appears at 5-fold overdigestion (5 units x 1 hour). Restriction enzyme exhibits star activity under certain ...
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#36EcoRI - NZYTech
Incubation temperature. 37 °C for one hour. Heat inactivation. 65 °C for 20 minutes. Activity in NZYTech Buffers. () weak star activity is detected. EcoRI.
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#37Restriction Enzymes EcoRI | Labettor
Star activity (or off-target cleavage) and incomplete cleavage are potential challenges which may occur due to suboptimal enzymatic conditions or ...
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#38QuickCut™ EcoR I
The digestion activity of each. QuickCut enzyme can reach 100% in the 10X QuickCut ... 1） To avoid star activity, the reaction time should be less than.
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#39Using proteases to control restriction enzyme activity - Justia ...
3-8 to indicate completion of cleavage and star activity. ... 3 : The enzyme activity of EcoRI on lambda DNA in buffers A1 and A1S2.
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#40In-Frame - GenScript
EcoRI. HindIII ... Star activity refers to the altered specificity of a ... Star activity can result in cleavage at non-canonical.
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#41High fidelity restriction endonucleases - European Patent Office
Star activity introduces undesirable cuts in a cloning vector or other DNA. ... Fig 7C: WT EcoRI in EcoRI buffer (see NEB catalog 2007-8).
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#42Molecular recognition mediated by bound water. A ...
By cleaving DNA with EcoRI in the presence of several osmolytes, it is shown that the extent of this EcoRI "star activity" depends strongly ...
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#43What is EcoRI? - Meaning, Structure, Uses, and FAQs For NEET
Learn about what is EcoRI topic of NEET in details explained by subject experts on ... are all conditions that can trigger star activity while using EcoRI.
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#44Restriction Endonuclease EcoRI Lab Flashcards | Quizlet
What are conditions that would cause star activity? high pH, high glycerol amounts, low ionic concentration.
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#45실험Q&A > 실험 > BRIC
제한효소처리를 하는데 Xho1 과 Ecor1 을 사용 하였습니다. ... EcoRI은 고농도의 enzyme을 사용할 경우, star activity가 나타나는 대표적인 enzyme ...
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#46Expression and purification of a single-chain Type IV ... - Nature
Perhaps the negatively regulatory loop byATP provides a safeguard to GmrSD star activity on unmodified DNA when GmrSDenzyme is “accidently” ...
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#47Biology:Star activity - HandWiki
Star activity is the relaxation or alteration of the specificity of ... by Mayer who characterized the modified activity in EcoRI.
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#48Molecular Biology of Nucleases - 第 69 頁 - Google 圖書結果
The enzyme with new specificity has been designated as EcoRI * ( star ) activity . Replacement of Mg2 + by Mn2 + as well as the presence of 40-50 % glycerol ...
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#49Molecular Biology: A Project Approach - 第 70 頁 - Google 圖書結果
Star activity (EcoRI”), where EcoRI cuts at altered sequences, can be observed in buffers other than the standard EcoRI buffer. Under star activity ...
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#50DNA Profiling - 第 73 頁 - Google 圖書結果
Excessive Digestion ( " Star ' Activity ) The second type of artifact that can ... This is known as ' Eco RI star ' ( Eco RI * ) activity : The recognition ...
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#51Recombinant DNA Methodology - 第 52 頁 - Google 圖書結果
Secondary (Star) Activity of Restriction Endonucleases Secondary (star) activity ... One prominent example is EcoRI, where the usual hexanucleotide sequence ...
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#52Frontiers in High Pressure Biochemistry and Biophysics
Robinson and Sligar report that the star activity of BamHI and Pvull , similar to that of EcoRI , correlates directly with osmotic pressure and that ...
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#53Recombinant DNA Principles and Methodologies
This latter phenomenon is referred to as star activity ( denoted by an asterisk after the enzyme name , e.g. , EcoRI “ ) . Star activity is the result of a ...
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#54Oxygen Responses, Reactivities, and Measurements in Biosystems
A number of laboratories have reported star activities of EcoRI. In general, star activity is observed under altered reaction conditions, ...
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#55Enzymology Primer for Recombinant DNA Technology
The star or secondary activity can also be exploited to cleave DNA molecules containing no canonical EcoRI site. (d) Relaxed conditions. Star activities can ...
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#56Topics to cover Biological origin and function of restriction ...
This results in a double-stranded break EcoRI crystal structure. Dimer bound to DNA. ... 16 „Star activity“ of restriction enzymes
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#57Solved Table A1 in Appendix 2 shows RE activities in various
Answer to Solved Table A1 in Appendix 2 shows RE activities in various. ... of reduced star activity, rapid digestion and 100% activity in NEB Buffer 4.1 No ...
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#58C3h5n hybridization Propanoic acid H. NH 3, Show the ...
The molecule below has been detected in gas clouds between stars. ... A 1n8 kb fragment was deleted from pAAV3-lacZ by digestion with EcoRI and EcoRV ...
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