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#1beta Actin (ACTB) Human qPCR Primer Pair (NM_001101)
beta Actin (ACTB) Human qPCR Primer Pair (NM_001101). CAT#: HP204660. Write a review. qSTAR qPCR primer pairs against Homo sapiens gene ACTB ...
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#2Table 1. Primer sequence for RT-PCR. ACTB - beta-actin - MDPI
Primer sequence for RT-PCR. ACTB ‒ beta-actin, HMGCR - HMG-CoA reductase, CPT1a - carnitine palmitoyltransferase I, CHKA - choline kinase alpha, ...
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#3Design and testing of beta-actin primers for RT-PCR that do ...
In this paper, we report the results of using three sets of beta-actin primers for RT-PCR in the presence and absence of genomic DNA. In addition, we propose ...
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#5β-Actin and GAPDH housekeeping gene expression ... - Thorax
The β-actin primers were forward primer GCA CCA CAC CTT CTA CAA TG and reverse primer TGC TTG CTG ATC CAC ATC TG. β-Actin PCR products were 619 bp from ...
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#6Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB ...
Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited) ... TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan™ Primers and Probes.
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#7BETA-ACTIN - PCR Primer Pair - SYBR | PrimePCR - Bio-Rad
PrimePCR™ SYBR® Green Assay: BETA-ACTIN, Pig ... Real-time PCR primer assay designed for SYBR ® Green gene expression analysis. ... Gene-specific PCR primers for the ...
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#8Human beta-Actin qPCR Primer Pair | Sino Biological
High specificity and sensitivity Human beta-Actin qPCR primer pair is designed by proprietary primer design algorithm and validated by strict process.
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#9Supplementary Table 1. Sequences of the primers used for RT ...
Sequences of the primers used for RT-PCR or real-time RT-PCR in this study. Mouse gene. Primer Sequence 5' to 3'. Forward. Reverse ... β-actin (mouse / rat).
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#10cDNA and amplicon sequence for beta-actin primers. The full ...
Download scientific diagram | cDNA and amplicon sequence for beta-actin primers. The full cDNA and amplicon sequences as well as the highlighted location of ...
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#11Selective measurement of α smooth muscle actin
9b). These results suggest that cross-reaction between ß-actin primer pairs and different actin isoforms, such as α-SMA in the fibrotic kidney, ...
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#12Supplemental Table 1. List of primers used for classical RT ...
For analysis, all RT-PCR products were run on 1.5% agarose gel containing ethidium bromide. 2.β-actin, PPIB (cyclophilin B) and RPL13A1 genes were used as ...
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#13a resource of human and mouse PCR primer pairs for gene ...
PrimerBank search results for beta-actin primers. The primer search function can be found on the PrimerBank homepage. The database was searched ...
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#141 Supplementary Table 1. Primer sequence
Primer sequence. Target Genes. Forward. Reverse. Beta actin (β-actin). 5'-TTCCAGCCTTCCTTCTTG-3'. 3'-GGAGCCAGAGCAGTAATC-5'.
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#15Primer Pair for Beta Actin (ACTB) | PGB340Mi01 - Cloud-Clone
PGB340Mi01, Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed | Products for research use only!
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#16Quantitative analysis of beta-actin, beta-2-microglobulin and ...
commonly used endogenous reference genes beta-actin (-actin), beta-2-microglobulin (2-MG) ... strand during extension of the primers via its 5′-3′.
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#17Product Manual Human beta actin RT-PCRmer™ - GeneLink
Human beta actin RT-PCR primer sets. Catalog No.: 40-1001-10, 40-101X-10. Store at –20oC. For research use only. Not for use in diagnostic procedures for ...
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#18Evaluation of gamma-actin, beta-actin, GAPDH, and 18S as ...
These primers are useful for gene expression research in canine mammary cancer. Key words: gamma-actin; GAPDH; endogenous control; female dogs; quantitative PCR ...
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#19Human ChIP-seq grade b-actin promoter primer ... - Diagenode
This primer pair specifically amplifies a genomic region containing the human beta actin (ACTB) promoter. The primers are thoroughly tested and optimized ...
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#20US8198052B2 - Primers for nucleic acid amplification in ...
Primers for amplification of the entire human β-actin gene are commercially available from Clontech Laboratories, Inc. (Palo Alto, Calif.) ...
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#21Mouse/Rat β-actin PCR Primer Pair - R&D Systems
Mouse/Rat β-actin PCR Primer Pair. Catalog Number: RDP-105 ... β-actin specific PCR∗ primers for RT-PCR (reverse transcription followed by polymerase chain.
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#22Downregulation of β-Actin Gene and Human Antigen R ... - IOVS
The two different primers sets showed a downregulation of β-actin gene in KC stroma compared with normal corneal stroma (Figs. 1B, 1C). When primer 2 was used ...
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#23A rapid real-time qRT-PCR assay for ovine ß-actin mRNA
primers and probes is based on the. hA. CTB . a. GeneBank accession no. U39357 for o vine ß-actin. mRN. A. b. M10277 for genomic human ß-actin.
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#24beta Actin (ACTB) Human qPCR Primer Pair (NM_001101)
HP204660 | beta Actin (ACTB) Human qPCR Primer Pair (NM_001101). ... Component, 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, ...
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#25All-in-One qPCR Primers for rat actin, beta (Actb) - BioCat GmbH
All-in-One qPCR Primers for rat actin, beta (Actb) ... By default, qPCR primer pairs are designed to measure the expression level of the splice variant ...
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#26SimpleChIP ® Human β-Actin 3' UTR Primers #13669
SimpleChIP ® Human β-Actin 3' UTR Primers contain a mix of forward and reverse PCR primers that are specific to the human β-actin 3' untranslated region. These ...
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#27Table S1 Sequences of primers used for quantitative RT-PCR ...
Primer. Sequences (5' to 3'). Mouse IFN-β Forward. AGGGCGGACTTCAAGATC. Mouse IFN-β Reverse ... Mouse β-actin Forward. GGCTGTATTCCCCTCCATCG.
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#28Amplification of human beta-actin gene by the reverse ...
fication of a housekeeping gene such as b-actin. In the present study we used specific oligonucleotide primers previously designed to amplify both the ...
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#29SUPPLEMENTAL MATERIAL Methods Primer Sequences ...
Rev: 5'-ATTTGTACCTGTGGGGAGGG. Mouse real-time PCR primers: β-actin ... stages (stage IIIA/B and IV)], stratified tumor sizes [grouped by smaller sizes (T1, ...
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#30Supplementary Table S1 Primer sequences and amplification ...
Waf1): Cyclin Dependent Kinase Inhibitor 1A; ACTB: Actin Beta. Supplementary Table S2 Primary antibodies used for western blot analysis.
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#31ACTB, Human actin, beta, Real Time PCR Primer Set
Buy ACTB, Human actin, beta, Real Time PCR Primer Set, item number: VHPS-110 from RealTimePrimers at Biomol!
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#32Evaluation of reference genes for real-time quantitative PCR ...
The most used HKGs in these studies include β-actin (Actin), β-tubulin ... RT-qPCR products generated with each set of primers were ...
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#33Protocol instruction: Test for the presence of the β-actin gene ...
The pair of primers used amplifies a PCR product of 181 bp, specific to the species Apis mellifera (GenBank accession no. AB023025). The β-actin gene is ...
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#34Onchocercal DNA amplification using beta actin gene primers ...
This study was to evaluate the diagnostic reliability of the beta actin gene primer pair to confirm its suitability for validating presence or ...
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#35Selection of housekeeping genes for quantitative gene ...
The gene expression of housekeeping genes (b-actin, ACTB; glyceraldehyde-3- ... dT primer 500 lg/mL, 1 lL of random primers, 1 lL.
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#36Reliable Detection of β–Actin Transcript from Human RNA ...
2004), with and without DNaseI treatment, to primers suggested to only recognise reverse transcribed β–actin and not pseudogenes (Primer set 2: forward 5′–cct ...
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#37Identification of the full-length β-actin sequence and ...
The amino acid sequence of β-actin in the tree shrew was compared to ... AF110103.1), we designed two gene-specific primers (GSP) for the ...
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#38Validation of Reference Genes for Normalization ... - Hindawi
Beta -actin (ACTB); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); ... and oligo-dT primers and Superscript II reverse transcriptase (Invitrogen Life ...
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#39Housekeeping Gene Selection Advisory - PLOS
Here, we show that GAPDH and β-actin are direct targets of miR-644a. ... Primers for real-time PCR were as follows: (i) GAPDH Forward ...
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#40Human qPCR Primer: ACTB|NM_001101.4|HQP016381
Validated All-in-One™ qPCR Primer for ACTB(NM_001101.4) [Search again] ... Gene Description: actin beta. Estimated Delivery: 1-3 weeks, but may vary.
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#41beta actin primer > BRIC
RT - PCR 용(?) beta actin primer 의 염기 서열이 필요 해서요.. 논문도 찾아보고 다른 고수님들이 답변해두신거도 봤는데, 다 size가 크다고.
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#42Supplementary Table 1. List of primers used in this study ...
Reverse primer. Rat Met. 5'-aggtcgcttcatgcaggt-3'. 5'-tccggagacacaggatgg-3'. Rat Runx1. 5'-cctccttgaaccactccact-3'. 5'-ctggatctgcctggcatc-3'. Rat Actin.
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#43Primer Pair for Beta Actin (ACTB) - USCN Business
PGB340Mi01, Primer Pair for Beta Actin (ACTB), Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed | Products ...
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#44Human beta-Actin Primer - Genekam
Latest News: 20.05.2022. Genekam developed real time PCR kit for detection of monkeypox virus: accurate, specific und sensitive.
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#45Alternative PCR protocol using a single primer set for ...
for highly conserved genes, such as beta-actin and beta- globin, have previously been used to detect DNA from.
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#46Cloning and sequence analysis of β-actin gene from Aedes ...
Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D ...
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#47Extended protocol and real-time PCR dataset for shrimp ...
Along with, beta-actin gene analysis using conserved primers was also performed in this article. Mitochondrial gene sequence as well as ...
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#48Untitled Document
在細胞能表現大量的B型肝炎表面抗原(HbsAg)的情況下，以免疫組織化學染色觀察細胞 ... 萃取的ML-14a細胞的mRNA，先以β-actin基因(一種housekeeping-gene)的primer做 ...
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#49Supplementary materials
Western blot. β-actin was used as a loading control. (B) The efficiency of S100A14 overexpression ... Species Forward Primer Sequence (5' to 3').
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#50Universal SYBR Green qPCR Protocol - Sigma-Aldrich
B ) Individual reactions are characterized by the cycle at which fluorescence ... Specific primers for PCR should be designed with the aid of primer design ...
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#51研究服務| 鼎捷生技有限公司
內控基因通常選用表達量相對恆定的Housekeeping Gene，如：GAPDH、β-actin等。通過同時對參比基因的實時檢測，可以對樣品之間由於起始細胞數不同、或RNA純化效率的不同 ...
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#52In β‐actin knockouts, epigenetic reprogramming and rDNA ...
The ChIP-Seq profiles for β-actin and NM1 across the rDNA were further validated by ChIP and real-time RT-qPCR with primers amplifying the gene ...
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#53Documents - Takara Bio
Lot Number; On Label iPS Efficiency Check qPCR Primer Set Certificate of ... Chinese Murine Arf Primer Mix SDS; Chinese Murine beta-Actin Primer Mix SDS ...
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#54QuantiTect Primer Assays - QIAGEN
QuantiTect Primer Assays enable reproducible quantification of RNA transcripts ... The kits prevent the formation of PCR artifacts (see part [B] of figure ...
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#55Human ChIP-seq grade b-actin promoter primer pair
Human ChIP-seq grade b-actin promoter primer pair. You must be logged in to be able to place orders. diagenode. Human ChIP-seq grade b-actin promoter primer ...
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#563') β-actin F TATTGGC
Supplemental Table 1 Primers List. qPCR Primer. Sequence (5'-3') β-actin F. TATTGGCAACGAGCGGTTCC β-actin R. GGCATAGAGGTCTTTACGGATGT.
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#57Optimization of semi-quantitative RT PCR analysis for CPT I ...
Iran), and in the presence of the specific primers for CPT I gene together with the β-actin gene, used as an internal control as described below.
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#58VARIABILITY OF ACTIN GENE EXPRESSION DURING ... - jstor
All tissue samples forward primer, 10 pi reverse primer and 1,400 pi of ... Beta-actin cytoskeleton, and is involved in many functions.
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#59Table S1. Sequences of primers used for quantitative real ...
Homo sapiens β-Actin. GGACTTCGAGCAAGAGATGG. AGCACTGTGTTGGCGTACAG. NM_001101.3. 234. Sequence of primers sets were designed by the Primer Express Software ...
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#60Human β-actin gene primer (for qPCR) AG11722 - 艾科瑞生物
Human β-actin gene primer (for qPCR) AG11722. ¥100.00. 管家基因经常在RT-qPCR 实验中被作为内参基因使用。本制品是干粉状的用于定量PCR 的Human 管家 ...
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#61Universal Primers Targeting β-actin Genes of Several Fish ...
We designed a universal primer set for quantitative polymerase chain reaction (qPCR) based on the nucleotide sequences of several fish β-actin genes for ...
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#62Quantification of fungal abundance on cultural heritage using ...
The DNA standards were generated with the β-actin primers, mentioned above, with the following PCR program: 95°C for 3 min and the 30 cycles of ...
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#63integration of beta-actin gene for sample quality check in hsv ...
Primers and probes for the in-vitro detection of Human HSV- 1 and HSV-2 virus in a sample comprising nucleotide sequences selected from the ...
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#64Supplemental Material and Methods Expression Plasmids ...
primer mixtures (Supplemental Table 2) were used for the real-time PCR. Standard curves ... were normalized against β-actin RNA. Student's t-test was used ...
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#65Differentiation and Detection of Sugar Beet Fungal Pathogens ...
presented above and below the primer sequence. (B) Diagrammatic representation of location of primers on a generic actin gene and rRNA gene. Most.
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#66ACTIN GENE FROM TRICHODERMA HARZIANUM
the amplification of β-actin gene, the genomic DNA was subjected to the polymerase chain reaction (PCR) by using sequence specific primers.
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#67pExT (Plasmid #36889) - Addgene
Cloning method Unknown; 5′ sequencing primer see sequence ... insertion of a loxP site; Promoter CAG (chicken beta actin promoter and CMV enhancer) ...
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#68PrimeTime qPCR Primer Assays - Integrated DNA Technologies
PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR ® Green (Molecular Probes) or EvaGreen ® ...
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#69Table S1: List of primers used for real time PCR
Real-Time PCR (Mus musculus). Gene. Fw primer. Rev primer β-actin. ATCAAGATCATTGCTCCTCCTGA. G. CTGCTTGCTGATCCACATCTG. Nfatc1. AACGCCCTGACCACCGATAGCA.
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#70▷ beta Actin Gene - genomics-online.com | cDNA | ORF | Kits
Compare & Order beta Actin plasmids, CDNA clones, ORF clones and more genomics products. ... Primer. filter_list Active filters: Gene "Actin, beta (ACTB)"
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#71Measuring Gene Expression from Mammalian Brain Tissue
Negative control reactions that contained NGF or β-Actin primers with no RNA resulted in no product (lanes 7 and 8). RT-PCR analysis of mouse and rat total RNA ...
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#72chicken beta-actin promoter Sequence and Map - SnapGene
Download chicken beta-actin promoter.dna file · Fast accurate construct design for all major molecular cloning techniques · Validate sequenced constructs using ...
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#73Cloning of beta-actin in early diverging vertebrate little skate ...
β -Actin (ACTB) is a ubiquitously expressed cytoskeletal protein. ... Gene specific primers (Table 1) targeting ACTB cDNA were designed based ...
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#74a resource of human and mouse PCR primer pairs for gene ...
Figure 1. PrimerBank search results for beta-actin primers. The primer search function can be found on the PrimerBank homepage.
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#75ACTB cDNA ORF clone, Homo sapiens(human) - GenScript
Next-day shipping cDNA ORF clones derived from ACTB actin beta available at GenScript, starting from $99.00.
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#76Optimizing qPCR sensitivity and specificity (WEBINAR)
Demonstrate the benefits of properly optimized primers in qPCR analysis. Exemplify the ease and use of GeneQuery qPCR arrays in gene expression ...
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#77Non-variola Orthopoxvirus Generic Real-Time PCR Test | CDC
Orthopoxvirus primers and probe (Table 1) b. Human DNA primers and probe (e.g., RNaseP, β-actin, Glyceraldehyde-3-phosphate.
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#78The Arp2/3 complex is required for lamellipodia extension and ...
S3 B), an actin-bundling protein found in filopodia (Vignjevic et al., 2006; ... with the following primers: P1: ARPC3-Wt-F 5′-TGCAGGCATACCACTCTTCTCTCA-3′; ...
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#79COVID-19 | Potential role of astrocyte angiotensin converting ...
ACE2 primer 2: Forward 5ʹ-TTTGTTTCTGTTGGCCTTCC-3ʹ, Reverse 5ʹ-CATTGGCTCCGTTTCTTAGC-3ʹ. β-actin was used for normalization.
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#80Evaluation of Maxwell® 16 for automated DNA extraction from ...
... as demonstrated by β-actin for whole blood specimens. ... The PCR reaction contained 200 nM of each primer, Ready-to-Use Takara Omnimix ...
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#81End-Stage Renal Disease Rats receiving Peritoneal Dialysis
由 X Li 著作 · 2022 — The nuclear factor kappa B (NF-κB) is a transcription factor family ... during the process. β-actin was used as an internal reference, ...
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#82Trex1 Exonuclease Degrades ssDNA to Prevent Chronic ...
exponential culture (Figure 2B), with an increased cell ... Actin is a control for ... manner would still contain the 50 RNA primer and therefore.
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#83Subcellular Localization of Rice Acyl-CoA-Binding Proteins ...
OsACBP4 or OsACBP5 gene-specific primers (ML1109/ML1110 for OsACBP4 and ML1111/ML1112 for OsACBP5), and the rice. ACTIN (ML1115/ML1116) was ...
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#84The Science Behind Foldit
Actin is one of the major proteins in our muscles. ... A protein called amyloid-beta precursor protein is a normal part of healthy, functioning nerve cells ...
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#85Flexible polymeric ultrathin film for mesenchymal stem cell ...
Probes and primers were selected using web- X-100 in PBS for 5 min. ... with well-defined actin filaments and marked stress fibers ori- ented along the ...
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#86Def of genetics - Autoankauf Diamant
Common terms and phrases. actin activity allele amino acid animal antigen Appendix ... 2 a : of, relating to, or involving genetics. b : of, relating to, ...
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#87فينكولين - ويكيبيديا
يوجد الفينكولين في خلايا الثدييّات وهو بروتين هيكلي خلوي يتوضّع في لويحات الالتصاق البؤريّة ... بالإنتغرين- β و يرتبط المِطراف الكربوكسيلي بالأكتين والشحوم الفوسفوريّة و ...
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#88Embryonic Stem Cell Protocols: Volume I: Isolation and ...
Test the RT templates first with the positive control primer pair (i.e., β-actin primers) before using the other gene-specific primers. Analyze the β-actin ...
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#89Multiple Drug Resistance in Cancer: Cellular, Molecular and ...
This is because the region amplified with the primers chosen for 3-actin has ... primer is slightly below the recommended range; the Topo II(b) primer is ...
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#90Advances in Biomedical and Molecular Neuroscience
Primer Sequence Supplier HCN1 GeneWorks Pty Ltd HCN2 GeneWorks Pty Ltd ... Ltd HCN4 GeneWorks Pty Ltd Beta Actin GeneWorks Pty Ltd eGFP GeneWorks Pty Ltd a ...
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#91Signal Transduction in the Retina - 第 286 頁 - Google 圖書結果
QPCR primer sets should be designed to amplify 100–200 bp amplicons, ... Additionally, GAPDH and/or β-actin should always be included in each run to allow ...
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#92Textbook of Diagnostic Microbiology - E-Book
19 18 17 16 15 HSV PC 14 13 12 β-actin PC 11 ) 10 9 1 F ( e cnecseroul F 8 ... of the internal region of the PCR amplicon during the primer-annealing step.
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#93Journal of the National Cancer Institute: JNCI.
The primer pairs employed were based on the sequence of human wild - type p53 ... gels and by western blot analysis of B - actin levels in each sample .
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#94Commonalities and Differences in Vestibular and Auditory ...
Primer efficiencies, specificity, and optimal cDNA Consequently, ... Hair cell each gene relative to the housekeeping gene β-Actin (ACTB). layers were not ...
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#95Environmental Health Perspectives: EHP.
... arbitrary primers ( primer A3 : 5'AATCTAGAGCTCTCCTGG - 3 ' ; primer B : 5 ... ( Chelydra serpentina ) B - actin ( 100 % identical at the predicted amino ...
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#96Insect Olfactory Proteins (From Gene Identification to ...
A first 41-cycle touchdown PCR was performed using 5' RACE outer primers ... and the β-actin gene (GenBank No: KF022227.1) were used as reference genes.
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