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[爆卦]7-AAD BioLegend是什麼？優點缺點精華區懶人包
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#17-AAD Viability Staining Solution - BioLegend
7 -AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and ...
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#27-AAD Viability Staining Solution | BioLegend 420403 product ...
7 -AAD Viability Staining Solution. catalog : 420403. quantity : 200 tests. price : 25 USD. more info or order : BioLegend product webpage. citations: 161 ...
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#37-AAD Viability Staining Solution from BioLegend - Biocompare
Compare 7-AAD Viability Staining Solution from BioLegend on Biocompare.com. ... 8999 BioLegend Way. San Diego, California 92121. United States.
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#4(640922) FITC Annexin V Apoptosis Detection Kit with 7-AAD
Kit [640922] FITC Annexin V Apoptosis Detection Kit with 7-AAD by BioLegend.
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#5eBioscience™ 7-AAD Viability Staining Solution - Thermo Fisher
7 -AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can be used in place of PI ...
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#6Biolegend 7-AAD Viability Staining Solution | Fisher Scientific
7 -AAD Viability Staining Solution Apps: FC; Size: 200 tests. Catalog No. NC9067577. $39.00 / Each. Qty Check Availability ...
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#77 Aad (BioLegend) | Bioz | Ratings For Life-Science Research
BioLegend 7aad. 35A Stimulation results in reduced viability in responding cells. P14 CD8+T cells were 7aad, supplied by BioLegend, used in various ...
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#8APC Annexin V Apoptosis Detection Kit with 7-AAD from BIOLEGEND
BioLegend's APC Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells.
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#97-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry ...
Read our Analysis of Cell Viability using 7-Amino Actinomycin D (7-AAD) to help with your experiment. Learn more.
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#10BioLegend Archives - Australian Biosearch
BioLegend develops world-class, cutting-edge antibodies and reagents for ... 7-AAD Viability Staining Solution, 420403, BioLegend, 200 tests, VIEW DETAILS.
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#11FITC Annexin V Apoptosis Detection Kit with 7-AAD - Labettor
BioLegend's FITC Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells.
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#12Viability - Flow Cytometry & Cell Sorting Facility - Larner ...
Add (final concentration) 1 µg/ml PI, 500-1000 ng/ml DAPI, 2.5 µm 7-AAD, ... Other viability staining solution. 7-AAD: BioLegend, ThermoFisher Sci,
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#13Reagents for Cell Death Detection Annexin V
7 -AAD. Treated. Visit biolegend.com for further information. Characterisation of apoptosis using the Annexin V assay: A. Viable cells: Negatively stained ...
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#14inline-supplementary-material-2.pdf
Human/Mouse. 7-AAD. BD Biosciences. 51-68981E. PE. T-bet. Human/Mouse. 4B10. eBiosciences. 12-5825-82. PE. GITR. Human/Mouse. 621. Biolegend. 311604.
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#15Apoptosis 측정 - Annexin V detection Kit[Biolegend] > BRIC
... plasma membrane integrity is lost, allowing live cell impermeant DNA dyes like Propidium Iodide (PI) and 7-Aminoactinomycin D (7-AAD) to stain.
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#16Biolegend cell staining buffer | Sigma-Aldrich
of cells stained with 7-AAD, resulting in an underestimation of the early apoptotic cells and an overestimation of the late apoptotic ...
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#174-Ethylguaiacol modulates neuroinflammation and Th1/Th17 ...
由 WT Weng 著作 · 2021 · 被引用 1 次 — In addition, cells were stained with Alexa Fluor 488-conjugated anti-mouse CD4 (clone: RM4-5, BioLegend) in the presence of 7-AAD followed ...
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#18Mojosort 對的人轉
BioLegend ® -MojoSort™. 磁珠細胞分選套組 ... Fig.7 MojoSort™ Mouse CD3 T Cell Isolation Kit (cat.no 480023) ... Dead cells were excluded by 7-AAD.
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#19Updated sup tab 7 - JCI Insight
Biolegend. 350516. Viability Dyes Concentration. Detection. Clone. Company. Catalog Number. 7-AAD. 20µl/1x106 Cells. 561nm 780/60 -. BD Biosciences 555816.
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#20BioLegend Live/Dead Cell Discrimination - Chromocyte
BioLegend provides DNA dyes, Helix NP™ NIR, DRAQ7™, Propidium Iodide and 7-AAD, that enter and stain dead cells, but are impermeable to live cells for rapid ...
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#21Pacific Blue™ Annexin V Apoptosis Detection Kit, Biolegend
Annexin V Apoptosis Detection Kit with 7-AAD, 100 Tests, 1, 2...8 °C, 640922, 10763-246, Each. Retrieving. Temperature sensitive ...
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#22PowerPoint 演示文稿 - Haematologica
ALL leukemia stem cells were sorted against CD34 (Biolegend Cat. ... B-ALL CD34+CD19+ cells viability was detected by staining of 7-AAD (Biolegend Cat.
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#23Cell Surface Flow Cytometry Staining Protocol(from Biolegend)
420301); 7-AAD Viability Staining Solution (BioLegend Cat. No. ... bone marrow) and prepare a single cell suspension in Cell Staining Buffer (BioLegend Cat.
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#24Anti-BrdU Staining Protocols Using DNAse with Surface and ...
Cell Staining BufferBioLegendCatalog #420201. 7-AAD Viability Staining SolutionBioLegendCatalog #420403, 420404.
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#25Multi-lineage Lung Regeneration by Stem Cell ...
Anti-mouse CD45R (B220) APC-Cy7 (Biolegend) FACS. 103224. 1ul/106 cells. 7-AAD (BD Pharmingen). FACS. 51-68981E. 1ul/106 cells. *Secondary antibodies.
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#26Buffers, Solutions, Substrates & Media Additives
BIOLEGEND (246.5343). Tel: 858.768.5800 ... BioLegend's ELISA Buffers & Solutions are quality-tested and provide ... 7-AAD Viability Staining Solution.
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#27Dead_Cell_Exclusion_Dyes
Biolegend. 423107. Dead. Fixable. Impermeant. Amino. LIVE/DEAD Fixable Aqua ... Biolegend. 423101. Dead. Fixable. Impermeant ... 7-AAD.
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#28Product Data Sheet
Blue™ and viability dye 7-AAD ... Wash cells twice with cold BioLegend cell staining buffer (cat # 420201).
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#29media-4.docx - bioRxiv
Pacific Blue™ anti-mouse CD4 Antibody, 100428, BioLegend ... 7-AAD Viability Staining Solution, 420404, BioLegend ...
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#30V500 Mouse anti-Human CD19 - BD Biosciences
The HIB19 monoclonal antibody specifically binds to the 95 kDa type I transmembrane CD19 glycoprotein. CD19 is expressed during all stages of B-cell ...
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#31Advertising (PDF)
BIOLEGEND (246.5343). Tel: 858.768.5800 biolegend.com. 08-0052-03 ... (145-2C11), and 7-AAD. Grateful Dead cells were excluded from the analysis.
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#32GSM3142419 - GEO Accession viewer
Growth protocol, 6-7 week old C57BL/6J female mice were purchased from The ... 7-AAD Viability Staining Solution (BioLegend, 420404).
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#33Product Data Sheet
Recommended Usage: For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and ...
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#34a-simple-and-rapid-alternative-to-51chromium.pdf - ATCC
actinomycin D (7-AAD; BioLegend) at 0.25 µg per sample for 10 minutes at ... GFP fluorescence was detected on FL1 and 7-AAD fluorescence was detected on FL3 ...
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#357-amino-actinomycin d staining of dead cells for flow
7-Amino-actinomycin D (7-AAD) intercalates into double-stranded nucleic acids. It is excluded by viable cells but can penetrate cell membranes of dying or dead ...
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#36Moody Foundation Flow Cytometry Facility
biolegend. 595. 610/20. D. Propodium Iodide. 540. 620. 595. 610/20. D. PE-Alexa Fluor 610. 488, 565. 628. Life Technology. 635. 670/30. C. 7-AAD.
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#37A Retinoid X Receptor Agonist Directed to the Large Intestine ...
Dead cells were labeled with 7-aminoactinomycin D (7-AAD) (BioLegend). CD45 + CD4 + CD45RB high live T cells were isolated by FACS Aria III (BD ...
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#38Irgm1 regulates metabolism and function in T cell subsets
Cells were collected and washed with FACS buffer and stained with PE-annexin V and 7-AAD (Biolegend) following the manufacturer protocol.
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#39標識抗体・試薬 BioLegend - トミーデジタルバイオロジー株式 ...
BioLegend 社は米国San Diegoに本社を置き、最先端のバイオロジー研究試薬を開発製造し ... 生死判定・アポトーシス測定としてAnnexin V Binding Buffer(422201)、7-AAD ...
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#40Bispecific NKG2D-CD3 and NKG2D-CD16 fusion proteins for ...
Dead cells were excluded from analysis with 7-AAD (BioLegend) or LIVE/DEAD™ Fixable Aqua (Thermo Fisher Scientific, Waltham, MA).
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#41فروشگاه آنلاین سما تشخیص آریا
7 -AAD Viability Staining Solution. Company: BioLegend. Catalog: 420403. Size: 200 tests. Storage & Handling: Protect from light. Store between 2°C and 8°C.
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#42A Retinoid X Receptor Agonist Directed to the Large Intestine ...
and 7-aminoactinomycin D (7-AAD) (BioLegend) and were analyzed by FACS Celesta and FlowJo software version 10 (BD. Biosciences). Histology.
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#43Circulating inflammatory monocytes oppose microglia and ...
2; Biolegend), and F4/80 (FITC-conjugated, clone; BM8 Biolegend). Dead cells were excluded with 7-AAD (BD Pharmingen). Microglia were defined as ...
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#44Differentiation into an Effector Memory Phenotype Potentiates ...
express the costimulatory molecule CD27 (7) as well as CD62L and CCR7, ... 560838), 7-aminoactinomycin D (7-AAD) (BioLegend 420403), and.
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#451 TITLE Anti-tumor activity without on-target off-tumor toxicity ...
7. Cell products at cell doses of up to 1x109 cells/m2 for dose level (DL) 5 were ... Cy7 (Biolegend) and 7-AAD viability dye (Beckman coulter).
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#46ARTICLE - Fibroblast growth factor receptor 5 (FGFR5) is a co ...
identified by flow cytometry using Annexin V/7-AAD staining ... with Annexin V–APC and 7-AAD (BioLegend) following the.
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#47MONOCYTE CELL SORTING FOR RNASEQ - Bowdish Lab
5mL polystyrene round bottom tubes with cell-strainer cap (352235). • 5mL polypropylene round bottom tubes (FACS Tubes). • BD Pharmingen 7 AAD (559925).
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#48Safety and efficacy of tisagenlecleucel in primary CNS ...
... markers PD-1-BV786, TIM-3-BV711, LAG-3-AF647, and a soluble CD19-PE detection reagent (BioLegend). 7-AAD was used to exclude dead cells.
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#49RIG-I acts as a tumor suppressor in melanoma via regulating ...
... with 7-AAD using a BrdU Cell Proliferation Kit (Biolegend, USA). The results were evaluated with FlowJo software (BD Bioscience, USA).
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#507-AAD Staining Solution | Apoptosis and cell viability - Miltenyi ...
The 7-AAD Staining Solution is a ready-to-use reagent suitable for the exclusion of dead and apoptotic cells from flow cytometric analysis. | USA.
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#51Anoikis resistant mediated by FASN promoted growth and ...
The PE Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and ... Approximately 2 × 10 7 Luc-143B and AR-Luc-143B cells (suspended in ...
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#52Concurrent Dexamethasone Limits the Clinical Benefit of ...
eBioscience) and anti-Ki67 (16A8, BioLegend), and anti-IFNg. (XMG1.2). ... annexin-V and 7-AAD staining) in nontumor-bearing mice eutha-.
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#53【7-AAD細胞活力螢光指示劑】7-Aminoactinomycin D Ready ...
7 -AAD與其母體分子Actinomycin D一樣，是具有生長抑制特性的DNA嵌入劑。7-AAD，PE和FITC螢光發射之間的光譜重疊最小， 激發/發射– 488/647 nm。產品Ready made solution ...
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#547-AAD [7-Aminoactinomycin D] *CAS 7240-37-1* | AAT Bioquest
7-Amino actinomycin D (7-AAD) is a non-permeant dye that can be used to identify non-viable cells.
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#55ReadiDrop™ 7-AAD | Bio-Rad Laboratories
Use ReadiDrop 7-AAD (7-aminoactinomycin D), a ready-to-use cell viability dye, for excluding dead cells in flow cytometric experiments.
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#56SouthernBiotech [DNA活性染劑7-AAD] 7-Aminonactinomycin ...
7-Aminoactinomycin D (7-AAD) DNA活性染劑美國SouthernBiotech公司貨號: 10042-01 品名: 7-Aminoantinomycin D (7-AA.
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#57Radiation and the Immune System: Current Knowledge and ...
... 0.5–1 × 105 tumor cells were blocked with Fc Block (anti-CD16/32 antibodies, Affymetrix, USA), stained with 7-Aminoactinomycin D (7-AAD Biolegend, USA), ...
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#58Metabolic Plasticity of Cancer - 第 86 頁 - Google 圖書結果
After incubation during 15min, 7aminoactinomycin D (7-AAD, BioLegend, 0.1μg/mL) in HEPES buffer was added. Flow cytometry was performed using a Becton ...
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#59HIV-Associated Immune Activation and Persistent Inflammation
The following antibodies were used for immunostaining to isolate cell subtypes: FITC-CD3, APC-cy7-CD8, APC-CD4, PE-cy7-CD14 and 7-AAD (Biolegend, San Diego, ...
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#60Immunomodulatory Effects of Drugs for Treatment of ...
After washing, cells were re-suspended in Annexin binding buffer and stained with Annexin V-FITC and 7-AAD (Biolegend, San Diego, CA, USA) following ...
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#61Immunotherapies Towards HIV Cure - 第 25 頁 - Google 圖書結果
Apoptosis Measurement PBMC viability was measured using a Pacific BlueTM Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend).
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#627 AAD reagent Products - MyBioSource
Annexin V-APC/7-AAD Apoptosis Detection Kit. Applications: Apoptosis. Jurkat cells were treated with 1uM Camptothecin and detected by ...